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Hyo Sang Lee
Oregon Health & Science University
8Publications
6H-index
914Citations
Publications 8
Newest
#1Jo Ann Janovick (OHSU: Oregon Health & Science University)H-Index: 36
#2M. David Stewart (UH: University of Houston)H-Index: 17
Last.Suhujey Lopez (UH: University of Houston)H-Index: 4
view all 17 authors...
Many diseases result from genetic mutations that cause protein misfolding. Medical treatments often address the symptoms, but do not correct the underlying etiology. This study illustrates proof of principle that a disease caused by a misfolded cell surface receptor can be corrected with a pharmacoperone, a unique class of target-specific drugs that assist protein folding.
#1Masahito Tachibana (Oregon National Primate Research Center)H-Index: 14
#2Paula Amato (OHSU: Oregon Health & Science University)H-Index: 21
Last.Hathaitip Sritanaudomchai (MU: Mahidol University)H-Index: 9
view all 23 authors...
Summary Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT)-ESCs for studies of disease mechanisms and for developing specific therapies. Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. Here, we identified premature exit from meiosis in human oocytes and suboptimal activation as key factors tha...
#1Masahito Tachibana (OHSU: Oregon Health & Science University)H-Index: 14
#2Paula Amato (OHSU: Oregon Health & Science University)H-Index: 21
Last.Hyo Sang Lee (OHSU: Oregon Health & Science University)H-Index: 6
view all 20 authors...
Mutations in mitochondrial DNA (mtDNA) are associated with severe human diseases and are maternally inherited through the egg’s cytoplasm. Here we investigated the feasibility of mtDNA replacement in human oocytes by spindle transfer (ST; also called spindle–chromosomal complex transfer). Of 106 human oocytes donated for research, 65 were subjected to reciprocal ST and 33 served as controls. Fertilization rate in ST oocytes (73%) was similar to controls (75%); however, a significant portion of S...
#1Masahito Tachibana (Oregon National Primate Research Center)H-Index: 14
#2Hong Ma (Oregon National Primate Research Center)H-Index: 27
Last.Yibing Jia (Oregon National Primate Research Center)H-Index: 1
view all 11 authors...
Inactivation of one X chromosome in female mammals (XX) compensates for the reduced dosage of X-linked gene expression in males (XY). However, the inner cell mass (ICM) of mouse preimplantation blastocysts and their in vitro counterparts, pluripotent embryonic stem cells (ESCs), initially maintain two active X chromosomes (XaXa). Random X chromosome inactivation (XCI) takes place in the ICM lineage after implantation or upon differentiation of ESCs, resulting in mosaic tissues composed of two ce...
#1Hyo Sang Lee (Oregon National Primate Research Center)H-Index: 6
#2Hong Ma (Oregon National Primate Research Center)H-Index: 27
Last.Paula Amato (OHSU: Oregon Health & Science University)H-Index: 21
view all 13 authors...
The timing and mechanisms of mitochondrial DNA (mtDNA) segregation and transmission in mammals are poorly understood. Genetic bottleneck in female germ cells has been proposed as the main phenomenon responsible for rapid intergenerational segregation of heteroplasmic mtDNA. We demonstrate here that mtDNA segregation occurs during primate preimplantation embryogenesis resulting in partitioning of mtDNA variants between daughter blastomeres. A substantial shift toward homoplasmy occurred in fetuse...
#1Masahito Tachibana (Oregon National Primate Research Center)H-Index: 14
#2Michelle Sparman (Oregon National Primate Research Center)H-Index: 14
Last.Shoukhrat Mitalipov (Oregon National Primate Research Center)H-Index: 36
view all 7 authors...
SUMMARY Totipotent cells in early embryos are progenitors of all stem cells and are capable of developing into a whole organism, including extraembryonic tissues such as placenta. Pluripotent cells in the inner cell mass (ICM) are the descendants of totipotent cells and can differentiate into any cell type of a body exceptextraembryonictissues.Theabilitytocontribute to chimeric animals upon reintroduction into host embryos is the key feature of murine totipotent and pluripotent cells. Here, we d...
#1Masahito Tachibana (Oregon National Primate Research Center)H-Index: 14
#2Michelle Sparman (Oregon National Primate Research Center)H-Index: 14
Last.Shoukhrat Mitalipov (OHSU: Oregon Health & Science University)H-Index: 36
view all 9 authors...
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