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Ying Zhang
University of Washington
Crystal structureMass spectrometryChemistryProtein-arginine deiminaseBiochemistry
29Publications
10H-index
405Citations
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Publications 29
Newest
#1Hong-Jun Cho (UIUC: University of Illinois at Urbana–Champaign)
#1Hong-Jun Cho (UIUC: University of Illinois at Urbana–Champaign)
Last. Liviu M. Mirica (UIUC: University of Illinois at Urbana–Champaign)H-Index: 28
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Alzheimer's disease (AD) is the most common neurodegenerative disease, and its main hallmark is the deposition of amyloid beta (Abeta) peptides. However, several clinical trials focusing on Abeta-targeting agents have failed recently, and thus new therapeutic leads are focusing on alternate targets such as tau protein pathology, Abeta-metal induced oxidative stress, and neuroinflammation. To address these different pathological aspects of AD, we have employed a multifunctional compound 4-(benzo[...
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#1Ying Zhang (WashU: Washington University in St. Louis)H-Index: 10
#2Aaron T. Wecksler (Genentech)H-Index: 16
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
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We previously analyzed the Fab-1:VEGF (vascular endothelial growth factor) system described in this work, with both native top-down mass spectrometry and bottom-up mass spectrometry (carboxyl-group or GEE footprinting) techniques. This work continues bottom-up mass spectrometry analysis using a fast photochemical oxidation of proteins (FPOP) platform to map the solution binding interface of VEGF and a fragment antigen binding region of an antibody (Fab-1). In this study, we use FPOP to compare t...
19 CitationsSource
#1Ying Zhang (Pfizer)H-Index: 10
#2Weidong Cui (WashU: Washington University in St. Louis)H-Index: 15
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
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Native mass spectrometry (MS) and top-down electron-capture dissociation (ECD) combine as a powerful approach for characterizing large proteins and protein assemblies. Here, we report their use to study an antibody Fab (Fab-1)–VEGF complex in its near-native state. Native ESI with analysis by FTICR mass spectrometry confirms that VEGF is a dimer in solution and that its complex with Fab-1 has a binding stoichiometry of 2:2. Applying combinations of collisionally activated dissociation (CAD), ECD...
9 CitationsSource
#1Ying Zhang (WashU: Washington University in St. Louis)H-Index: 10
#2Don L. Rempel (WashU: Washington University in St. Louis)H-Index: 15
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
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2 CitationsSource
#1Ying Zhang (WashU: Washington University in St. Louis)H-Index: 10
#2Don L. Rempel (WashU: Washington University in St. Louis)H-Index: 15
Last. Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 4 authors...
Unlike small-molecule drugs, the size and dynamics of protein therapeutics challenge existing methods for assessing their high order structures (HOS). To extend fast photochemical oxidation of proteins (FPOP) to protein therapeutics, we modified its platform by introducing a mixing step prior to laser irradiation to minimize unwanted H2O2-induced oxidation. This improvement plus standardizing each step yield better reproducibility as determined by a fitting process whereby we used a non-FPOP spe...
16 CitationsSource
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 11
Last. Paul R. ThompsonH-Index: 50
view all 9 authors...
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#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 11
Last. Paul R. ThompsonH-Index: 50
view all 9 authors...
Source
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 11
Last. Paul R. ThompsonH-Index: 50
view all 9 authors...
Source
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 11
Last. Paul R. ThompsonH-Index: 50
view all 9 authors...
Source
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 11
Last. Paul R. ThompsonH-Index: 50
view all 9 authors...
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