Match!
Y. Zhang
Washington University in St. Louis
31Publications
11H-index
402Citations
Publications 31
Newest
#1Y. Zhang (WashU: Washington University in St. Louis)H-Index: 11
#2Aaron T. Wecksler (Genentech)H-Index: 7
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 5 authors...
We previously analyzed the Fab-1:VEGF (vascular endothelial growth factor) system described in this work, with both native top-down mass spectrometry and bottom-up mass spectrometry (carboxyl-group or GEE footprinting) techniques. This work continues bottom-up mass spectrometry analysis using a fast photochemical oxidation of proteins (FPOP) platform to map the solution binding interface of VEGF and a fragment antigen binding region of an antibody (Fab-1). In this study, we use FPOP to compare t...
16 CitationsSource
#1Y. Zhang (Pfizer)H-Index: 11
#2Weidong Cui (WashU: Washington University in St. Louis)H-Index: 15
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 7 authors...
Native mass spectrometry (MS) and top-down electron-capture dissociation (ECD) combine as a powerful approach for characterizing large proteins and protein assemblies. Here, we report their use to study an antibody Fab (Fab-1)–VEGF complex in its near-native state. Native ESI with analysis by FTICR mass spectrometry confirms that VEGF is a dimer in solution and that its complex with Fab-1 has a binding stoichiometry of 2:2. Applying combinations of collisionally activated dissociation (CAD), ECD...
9 CitationsSource
#1Y. Zhang (WashU: Washington University in St. Louis)H-Index: 11
#2Don L. Rempel (WashU: Washington University in St. Louis)H-Index: 12
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 3 authors...
1 CitationsSource
#1Daniel J. SladeH-Index: 11
#2Pengfei FangH-Index: 12
Last.Paul R. ThompsonH-Index: 50
view all 12 authors...
Protein arginine deiminases (PADs) are calcium-dependent histone-modifying enzymes whose activity is dysregulated in inflammatory diseases and cancer. PAD2 functions as an Estrogen Receptor (ER) coactivator in breast cancer cells via the citrullination of histone tail arginine residues at ER binding sites. Although an attractive therapeutic target, the mechanisms that regulate PAD2 activity are largely unknown, especially the detailed role of how calcium facilitates enzyme activation. To gain in...
43 CitationsSource
#1Y. Zhang (WashU: Washington University in St. Louis)H-Index: 11
#2Don L. Rempel (WashU: Washington University in St. Louis)H-Index: 12
Last.Michael L. Gross (WashU: Washington University in St. Louis)H-Index: 75
view all 4 authors...
Unlike small-molecule drugs, the size and dynamics of protein therapeutics challenge existing methods for assessing their high order structures (HOS). To extend fast photochemical oxidation of proteins (FPOP) to protein therapeutics, we modified its platform by introducing a mixing step prior to laser irradiation to minimize unwanted H2O2-induced oxidation. This improvement plus standardizing each step yield better reproducibility as determined by a fitting process whereby we used a non-FPOP spe...
15 CitationsSource
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 10
Last.Paul R. ThompsonH-Index: 50
view all 9 authors...
Source
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 10
Last.Paul R. ThompsonH-Index: 50
view all 9 authors...
Source
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 10
Last.Paul R. ThompsonH-Index: 50
view all 9 authors...
Source
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 10
Last.Paul R. ThompsonH-Index: 50
view all 9 authors...
Source
#1Daniel J. SladeH-Index: 11
#2Xuesen ZhangH-Index: 10
Last.Paul R. ThompsonH-Index: 50
view all 9 authors...
Source
1234