Sandra Bosshard
University of Lausanne
Homologous recombinationDNA repairGenome editingChinese hamster ovary cellBiology
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Publications 4
#1Pierre-Olivier Duroy (UNIL: University of Lausanne)H-Index: 1
#2Sandra Bosshard (UNIL: University of Lausanne)H-Index: 2
Last. Nicolas Mermod (UNIL: University of Lausanne)H-Index: 35
view all 12 authors...
The Chinese hamster ovary (CHO) cells used to produce biopharmaceutical proteins are known to contain type-C endogenous retrovirus (ERV) sequences in their genome and to release retroviral-like particles. Although evidence for their infectivity is missing, this has raised safety concerns. As the genomic origin of these particles remained unclear, we characterized type-C ERV elements at the genome, transcriptome, and viral particle RNA levels. We identified 173 type-C ERV sequences clustering int...
1 CitationsSource
#1Sandra Bosshard (UNIL: University of Lausanne)H-Index: 2
#2Pierre-Olivier Duroy (UNIL: University of Lausanne)H-Index: 1
Last. Nicolas Mermod (UNIL: University of Lausanne)H-Index: 35
view all 3 authors...
Abstract CRISPR technologies greatly foster genome editing in mammalian cells through site-directed DNA double strand breaks (DSBs). However, precise editing outcomes, as mediated by homologous recombination (HR) repair, are typically infrequent and outnumbered by undesired genome alterations. By using knockdown and overexpression studies in Chinese hamster ovary (CHO) cells as well as characterizing repaired DNA junctions, we found that efficient HR-mediated genome editing depends on alternativ...
2 CitationsSource
#1Kaja Kostyrko (UNIL: University of Lausanne)H-Index: 7
#2Samuel Neuenschwander (Swiss Institute of Bioinformatics)H-Index: 12
Last. Nicolas Mermod (UNIL: University of Lausanne)H-Index: 35
view all 12 authors...
Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous recombination (HR) DNA repair pathways. However, a direct involvement of these pathways has remained unclear. Here, we show that the silencing of many HR factors enhanced plasmid concatemer formation and stable expression of the gene of interest...
7 CitationsSource
#1Kaja KostyrkoH-Index: 7
#2Sandra BosshardH-Index: 2
Last. Nicolas MermodH-Index: 35
view all 4 authors...
Eukaryotic cells respond to DNA breaks, especially double-stranded breaks (DSBs), by activating the DNA damage response (DDR), which encompasses DNA repair and cell cycle checkpoint signaling. The DNA damage signal is transmitted to the checkpoint machinery by a network of specialized DNA damage-recognizing and signal-transducing molecules. However, recent evidence suggests that DNA repair proteins themselves may also directly contribute to the checkpoint control. Here, we investigated the role ...
11 CitationsSource