Margaret B. Aydelotte
Rush University Medical Center
Publications 21
Culture of articular chondrocytes in alginate beads offers several advantages over culture in monolayer; cells retain their phenotype for 8 months or longer. Earlier studies of chondrocytes cultured in alginate concentrated on collagen and proteoglycan synthesis. However, gene expression by in situ hybridization (ISH) has not been investigated. The purposes of the present study on human chondrocytes were (a) to modify the ISH procedure for the alginate beads to examine the mRNA expression of α1 ...
59 CitationsSource
#1Brian E. Pfister (Rush University Medical Center)H-Index: 1
#2Margaret B. Aydelotte (Rush University Medical Center)H-Index: 17
Last.Thomas M. Schmid (Rush University Medical Center)H-Index: 31
view all 5 authors...
Abstract Articular cartilage contains four distinct zones, extending from the surface to the subchondral bone. Freshly isolated chondrocytes from the superficial zone of articular cartilage retain a collagenase-P-resistant cell-associated matrix. In the studies described here, the protein Del1 was identified as a component of the cell-associated matrix of superficial zone chondrocytes from adult bovine articular cartilage. Very little Del1 was associated with freshly isolated deep zone chondrocy...
27 CitationsSource
#1David L. Reid (Rush University Medical Center)H-Index: 2
#2Margaret B. Aydelotte (Rush University Medical Center)H-Index: 17
Last.Jürgen Mollenhauer (Rush University Medical Center)H-Index: 3
view all 3 authors...
The purpose of this study was to investigate collagen receptors on primary bovine articular chondrocytes from full-thickness and different layers of bovine articular cartilage. Cytometric studies with antibodies showed that approximately 56% of the chondrocytes from the superficial layer and 29% of the chondrocytes from the deep layer bound anti-annexin V. A similar tendency was found for α and β1 integrin antibodies. Flow cytometric analysis initially detected annexin V on chondrocytes followin...
44 CitationsSource
#1Carl R. Flannery (Cardiff University)H-Index: 38
#2Clare Elizabeth Hughes (Cardiff University)H-Index: 39
Last.Bruce Caterson (Cardiff University)H-Index: 61
view all 7 authors...
Abstract We have performed cDNA sequencing and homology analyses to elucidate the complete amino acid composition for a superficial zone protein (SZP) from human and bovine cartilage which has previously been shown to be a proteoglycan specifically synthesized by chondrocytes located at the surface of bovine articular cartilage and also some synovial lining cells. The results of this study indicate that cartilage SZP is homologous with a glycoprotein first described as the precursor protein of a...
294 CitationsSource
#1Barbara L. Schumacher (Rush University Medical Center)H-Index: 15
#2Clare Elizabeth Hughes (Cardiff University)H-Index: 39
Last.Margaret B. Aydelotte (Rush University Medical Center)H-Index: 17
view all 5 authors...
We have previously described a large proteoglycan named superficial zone protein that was isolated and purified from culture medium of superficial slices of bovine articular cartilage. Monoclonal antibodies were raised against superficial zone protein and used as probes in Western blot analyses for immunohistochemical studies both to determine precisely which cells within the joint synthesize the proteoglycan and to isolate a cDNA fragment from a bovine chondrocyte λgt11 library that encodes par...
194 CitationsSource
The potentialities of polarization microscopy has been greatly increased by using specific stains for selective enhancement of the optical anisotropy of a macromolecular constituent of cells and tissues. Such stainings have proved to be especially useful in exploring the spatial orientation pattern of the extracellular matrix components. The retardation value, which characterizes quantitatively the degree of submicroscopic orientation, can be measured traditionally with a compensator plate. This...
11 CitationsSource
#1Margaret B. Aydelotte (Rush University Medical Center)H-Index: 17
#2Eugene J.-M.A. Thonar (Rush University Medical Center)H-Index: 50
Last.Johannes Flechtenmacher (Rush University Medical Center)H-Index: 4
view all 4 authors...
Sodium alginate, which gels in the presence of calcium ions, is commonly used for culture of anchorage-independent cells, such as chondrocytes. Normally, the gel appears microscopically homogeneous but, depending on the conditions of gelation, it may contain a varying number of small channels that extend inward from the surface. We have examined the influence of these channels on the morphology of cultured chondrocytes entrapped in alginate beads. Growth-plate or articular chondrocytes cultured ...
37 CitationsSource
#1Klaus Huch (University of Ulm)H-Index: 19
#2Johannes Flechtenmacher (University of Ulm)H-Index: 4
Last.T. Kuber SampathH-Index: 2
view all 9 authors...
Objective. Recombinant human osteogenic protein 1 (OP-1) is an effective stimulator of human cartilage 35S-proteoglycan synthesis. The present study was conducted to determine whether stimulation of human articular chondrocytes with OP-1 can help overcome interleukin-1β (IL-1β)-induced suppression of 35S-proteoglycan synthesis. Methods. Human articular chondrocytes in alginate beads were maintained for 3 days in the absence (control) or presence of IL-1β at 0.1–100 pg/ml with or without OP-1 at ...
93 CitationsSource
#1Johannes Flechtenmacher (University of Ulm)H-Index: 4
#2Klaus Huch (University of Ulm)H-Index: 19
Last.Klaus E. Kuettner (Rush University Medical Center)H-Index: 48
view all 10 authors...
Objective. To study the effects of recombinant human osteogenic protein-1 (rHuOP-1; bone morphogenetic protein-7) on proteoglycan and collagen synthesis by human articular chondrocytes. Methods. Articular chondrocytes from fetal, adolescent, and adult human donors were cultured in alginate beads for 4 days in a mixture of Ham's F-12, Dulbecco's modified Eagle's medium, 10% fetal bovine serum (FBS), then for an additional 3-10 days in the presence and absence of rHuOP-1, with and without FBS. Cho...
217 CitationsSource
It has been demonstrated previously that interleukin-1 (IL-1) induces articular cartilage explants and chondrocytes in culture to produce elevated levels of inflammatory mediators such as interleukin-6 (IL-6) and prostaglandins. Previous studies have also demonstrated a relationship between IL-6 sec
6 CitationsSource