Abstract Metabolism in Ralstonia solanacearum , which causes lethal wilt on Solanaceous plants, is poorly understood. In this study, we selected a Tn5-inserted mutant of R. solanacearum SL341 showing various phenotypic changes and altered virulence. When the gdhA gene encoding NAD(P) + -dependent glutamate dehydrogenase was disrupted, the gdhA mutant of SL341 (SL341P2) was defective in red colony development on tetrazolium chloride-amended medium and showed less extracellular polysaccharide (EPS...
This study was conducted to evaluate tomato plant resistance against bacterial wilt by Ralstonia solanacearum using tomato cultivars or tomato breeding lines maintained in RDA-Genebank of Rural Development Administration and to select resistant tomato lines for breeding purpose. We evaluated the disease responses of a total of 13 cultivars and 39 breeding lines from RDA-Genebank using R. solanacearum SL341 strain, which is a representative strain in Korea. Tomato cultivar Hawaii 7996 and Moneyma...
Ralstonia solanacearum is the causal agent of bacterial wilt on a wide variety of plants, and enters a viable but nonculturable (VBNC) state under stress conditions in soil and water. Here, we adopted an artificial soil microcosm (ASM) to investigate the VBNC state of R. solanacearum induced by low temperature. The culturability of R. solanacearum strains SL341 and GMI1000 rapidly decreased at 4°C in modified ASM (mASM), while it was stably maintained at 25°C in mASM. We hypothesized that bacter...