Thomas Gensch
Forschungszentrum Jülich
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Publications 94
#1Jochen Dobner (HHU: University of Düsseldorf)H-Index: 4
#2Indra M. Simons (HHU: University of Düsseldorf)H-Index: 2
Last. Silke Hoffmann (Forschungszentrum Jülich)H-Index: 20
view all 11 authors...
The γ-aminobutyric acid type A receptor-associated protein (GABARAP) and its close paralogs GABARAPL1 and GABARAPL2 constitute a subfamily of the autophagy-related 8 (Atg8) protein family. Being associated with a variety of dynamic membranous structures of autophagic and non-autophagic origin, Atg8 proteins functionalize membranes by either serving as docking sites for other proteins or by acting as membrane tethers or adhesion factors. In this study, we describe that deficiency for GABARAP alon...
1 CitationsSource
#1Xiaoming Fan (CAS: Chinese Academy of Sciences)
#2Johnny Hendriks (Forschungszentrum Jülich)H-Index: 2
Last. Thomas Gensch (Forschungszentrum Jülich)H-Index: 22
view all 6 authors...
Single molecule localization microscopy (SMLM) allows the imaging of cellular structures with resolutions five to ten times below the diffraction limit of optical microscopy. It was originally introduced as a two-dimensional technique based on the localization of single emitters as projection onto the x-y imaging plane. The determination of the axial position of a fluorescent emitter is only possible by additional information. Here we report a method (spatial filter SMLM (SFSMLM)) that allows to...
#1Andrey O. Bogorodskiy (MIPT: Moscow Institute of Physics and Technology)H-Index: 4
#2Elena L Bolkhovitina (RAS: Russian Academy of Sciences)H-Index: 2
Last. Alexander M. Sapozhnikov (RAS: Russian Academy of Sciences)H-Index: 10
view all 12 authors...
People are constantly exposed to airborne fungal spores, including Aspergillus fumigatus conidia that can cause life-threatening conditions in immunocompromised patients or acute exacerbations in allergics. However, immunocompetent hosts do not exhibit mycoses or systemic inflammation, due to the sufficient but not excessive antifungal immune response that prevent fungal invasion. Intraepithelial dendritic cells (IE-DCs) of the conducting airway mucosa are located in the primary site of the inha...
#1Jan Meyer (HHU: University of Düsseldorf)H-Index: 2
#2Verena Untiet (Forschungszentrum Jülich)H-Index: 6
Last. Christine R. Rose (HHU: University of Düsseldorf)H-Index: 35
view all 5 authors...
Fluorescence lifetime imaging microscopy (FLIM) with fluorescent ion sensors enables the measurement of ion concentrations based on the detection of photon emission events after brief excitation with a pulsed laser source. In contrast to intensity-based imaging, it is independent of dye concentration, photobleaching, or focus drift and has thus been successfully employed for quantitative analysis of, e.g., calcium levels in different cell types and cellular microdomains. Here, we tested the suit...
4 CitationsSource
#1Iman AbdollahzadehH-Index: 2
#2Johnny HendriksH-Index: 16
Last. Thomas GenschH-Index: 22
view all 8 authors...
Subcellular structures containing autophagy-related proteins of the Atg8 protein family have been investigated with conventional wide-field fluorescence and single molecule localisation microscopy. Fusion proteins of GABARAP and LC3B, respectively, with EYFP were overexpressed in HEK293 cells. While size distributions of structures labelled by the two proteins were found to be similar, shape distributions appeared quite disparate, with EYFP-GABARAP favouring circular structures and elliptical st...
#1Sandra Burgstaller (Medical University of Graz)H-Index: 4
#2Helmut Bischof (Medical University of Graz)H-Index: 8
Last. Roland Malli (Medical University of Graz)H-Index: 34
view all 15 authors...
Distinct subcellular pH levels, especially in lysosomes and endosomes, are essential for the degradation, modification, sorting, accumulation, and secretion of macromolecules. Here, we engineered a novel genetically encoded pH probe by fusing the pH-stable cyan fluorescent protein (FP) variant, mTurquoise2, to the highly pH-sensitive enhanced yellow fluorescent protein, EYFP. This approach yielded a ratiometric biosensor—referred to as pH-Lemon—optimized for live imaging of distinct pH condition...
4 CitationsSource
#1Christian Rodenbücher (Forschungszentrum Jülich)H-Index: 11
#2Stephan Menzel (Forschungszentrum Jülich)H-Index: 27
Last. Krzysztof Szot (Forschungszentrum Jülich)H-Index: 19
view all 7 authors...
Electroreduction experiments on metal oxides are well established for investigating the nature of the material change in memresistive devices, whose basic working principle is an electrically-induced reduction. While numerous research studies on this topic have been conducted, the influence of extended defects such as dislocations has not been addressed in detail hitherto. Here, we show by employing thermal microscopy to detect local Joule heating effects in the first stage of electroreduction o...
3 CitationsSource
#1María Belén Rivas Aiello (UNLP: National University of La Plata)H-Index: 3
#2Daniel Castrogiovanni (CONICET: National Scientific and Technical Research Council)H-Index: 11
Last. Daniel O. Mártire (UNLP: National University of La Plata)H-Index: 26
view all 8 authors...
: Riboflavin (Rf) is an endogenous photosensitizer, which can participate in Type I and Type II processes. We have recently shown that the yield of the triplet excited states of Rf is enhanced in the presence of pectin-coated silver nanoparticles (Pec@AgNP) due to formation of a complex between Rf and Pec@AgNP (Rf-Pec@AgNP). Consequently, under aerobic conditions, the amounts of singlet molecular oxygen and superoxide radical anion generated are also larger in the presence of the nanoparticles. ...
3 CitationsSource
#1Stephan Endres (HHU: University of Düsseldorf)H-Index: 5
#2Marcus Wingen (HHU: University of Düsseldorf)H-Index: 6
Last. Thomas Drepper (HHU: University of Düsseldorf)H-Index: 26
view all 12 authors...
Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been eng...
5 CitationsSource
#1Rachel Conrad (Forschungszentrum Jülich)H-Index: 2
#2Gabriel Stölting (Forschungszentrum Jülich)H-Index: 9
Last. Patricia Hidalgo (HHU: University of Düsseldorf)H-Index: 2
view all 8 authors...
Summary Calcium entry through Ca V 1.2 L-type calcium channels regulates cardiac contractility. Here, we study the impact of exocytic and post-endocytic trafficking on cell surface channel abundance in cardiomyocytes. Single-molecule localization and confocal microscopy reveal an intracellular Ca V 1.2 pool tightly associated with microtubules from the perinuclear region to the cell periphery, and with actin filaments at the cell cortex. Channels newly inserted into the plasma membrane become in...
6 CitationsSource