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Masakazu Hashimoto
Osaka University
18Publications
7H-index
359Citations
Publications 18
Newest
Published on 2019in Developmental Cell9.19
Masakazu Hashimoto7
Estimated H-index: 7
(Osaka University),
Hiroshi Sasaki51
Estimated H-index: 51
(Osaka University)
Summary The epiblast is a pluripotent cell population first formed in preimplantation embryos, and its quality is important for proper development. Here, we examined the mechanisms of epiblast formation and found that the Hippo pathway transcription factor TEAD and its coactivator YAP regulate expression of pluripotency factors. After specification of the inner cell mass, YAP accumulates in the nuclei and activates TEAD. TEAD activity is required for strong expression of pluripotency factors and...
Published on Mar 1, 2017in Developmental Cell9.19
Katsura Minegishi3
Estimated H-index: 3
(Osaka University),
Masakazu Hashimoto7
Estimated H-index: 7
(Osaka University)
+ 16 AuthorsYasushi Okada42
Estimated H-index: 42
(RIKEN Quantitative Biology Center)
Summary Polarization of node cells along the anterior-posterior axis of mouse embryos is responsible for left-right symmetry breaking. How node cells become polarized has remained unknown, however. Wnt5a and Wnt5b are expressed posteriorly relative to the node, whereas genes for Sfrp inhibitors of Wnt signaling are expressed anteriorly. Here we show that polarization of node cells is impaired in Wnt5a –/– Wnt5b –/– and Sfrp mutant embryos, and also in the presence of a uniform distribution of Wn...
Published on Nov 8, 2016in The Molecular Biology Society of Japan
Masakazu Hashimoto7
Estimated H-index: 7
(Osaka University),
Yusuke Takenoshita (Osaka University)+ 1 AuthorsHiroshi Sasaki51
Estimated H-index: 51
(Osaka University)
Published on Oct 1, 2016in Developmental Biology2.94
Masakazu Hashimoto7
Estimated H-index: 7
(Osaka University),
Yukiko Yamashita2
Estimated H-index: 2
(University of Tokushima),
Tatsuya Takemoto13
Estimated H-index: 13
(University of Tokushima)
Abstract The CRISPR/Cas9 system is a powerful tool for elucidating the roles of genes in a wide variety of organisms including mice. To obtain genetically modified embryos or mice by this method, Cas9 mRNA and sgRNA are usually introduced into zygotes by microinjection or electroporation. However, most mutants generated with this method are genetically mosaic, composed of several types of cells carrying different mutations, which complicates phenotype analysis in founder embryos or mice. To simp...
Published on Sep 1, 2016in Science Advances
Fuminori Tanihara9
Estimated H-index: 9
(University of Tokushima),
Tatsuya Takemoto13
Estimated H-index: 13
(University of Tokushima)
+ 16 AuthorsShoichiro Sembon8
Estimated H-index: 8
Genetically modified pigs for biomedical applications have been mainly generated using the somatic cell nuclear transfer technique; however, this approach requires complex micromanipulation techniques and sometimes increases the risks of both prenatal and postnatal death by faulty epigenetic reprogramming of a donor somatic cell nucleus. As a result, the production of genetically modified pigs has not been widely applied. We provide a simple method for CRISPR (clustered regularly interspaced sho...
Published on Jul 1, 2016in Cell Reports7.82
Hidekazu Ishida9
Estimated H-index: 9
(Osaka University),
Rie Saba10
Estimated H-index: 10
(QMUL: Queen Mary University of London)
+ 18 AuthorsStephen C. Harmer9
Estimated H-index: 9
(QMUL: Queen Mary University of London)
A surface marker that distinctly identifies cardiac progenitors (CPs) is essential for the robust isolation of these cells, circumventing the necessity of genetic modification. Here, we demonstrate that a Glycosylphosphatidylinositol-anchor containing neurotrophic factor receptor, Glial cell line-derived neurotrophic factor receptor alpha 2 (Gfra2), specifically marks CPs. GFRA2 expression facilitates the isolation of CPs by fluorescence activated cell sorting from differentiating mouse and huma...
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