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Masakazu Hashimoto
Osaka University
Basal bodyWnt signaling pathwayGeneticsBiologyCell biology
21Publications
8H-index
514Citations
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Publications 20
Newest
#1Justine Ngo (Case Western Reserve University)H-Index: 5
#2Masakazu Hashimoto (Osaka University)H-Index: 8
Last. Anthony Wynshaw-Boris (Case Western Reserve University)H-Index: 87
view all 4 authors...
Abstract The Dishevelled proteins transduce both canonical Wnt/β-catenin and non-canonical Wnt/planar cell polarity (PCP) signaling pathways to regulate many key developmental processes during embryogenesis. Here, we disrupt both canonical and non-canonical Wnt pathways by targeting the entire Dishevelled family of genes (Dvl1, Dvl2, and Dvl3) to investigate their functional roles in the early embryo. We identified several defects in anterior-posterior axis specification and mesoderm patterning ...
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#1Masakazu Hashimoto (Osaka University)H-Index: 8
#2Hiroshi Sasaki (Osaka University)H-Index: 43
Last. Hiroshi Sasaki (Osaka University)H-Index: 24
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Summary The epiblast is a pluripotent cell population first formed in preimplantation embryos, and its quality is important for proper development. Here, we examined the mechanisms of epiblast formation and found that the Hippo pathway transcription factor TEAD and its coactivator YAP regulate expression of pluripotency factors. After specification of the inner cell mass, YAP accumulates in the nuclei and activates TEAD. TEAD activity is required for strong expression of pluripotency factors and...
17 CitationsSource
#1Masakazu HashimotoH-Index: 8
Last. Hiroshi SasakiH-Index: 24
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#1Katsura Minegishi (Osaka University)H-Index: 3
#2Masakazu Hashimoto (Osaka University)H-Index: 8
Last. Hiroshi Hamada (Osaka University)H-Index: 61
view all 19 authors...
Summary Polarization of node cells along the anterior-posterior axis of mouse embryos is responsible for left-right symmetry breaking. How node cells become polarized has remained unknown, however. Wnt5a and Wnt5b are expressed posteriorly relative to the node, whereas genes for Sfrp inhibitors of Wnt signaling are expressed anteriorly. Here we show that polarization of node cells is impaired in Wnt5a –/– Wnt5b –/– and Sfrp mutant embryos, and also in the presence of a uniform distribution of Wn...
30 CitationsSource
#1Masakazu Hashimoto (Osaka University)H-Index: 8
#2Yusuke Takenoshita (Osaka University)
Last. Hiroshi Sasaki (Osaka University)H-Index: 24
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#1Kenta YashiroH-Index: 22
#2Hidekazu IshidaH-Index: 9
Last. Ken SuzukiH-Index: 33
view all 15 authors...
#1Masakazu Hashimoto (Osaka University)H-Index: 8
#2Yukiko Yamashita (University of Tokushima)H-Index: 2
Last. Tatsuya Takemoto (University of Tokushima)H-Index: 14
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The CRISPR/Cas9 system is a powerful tool for elucidating the roles of genes in a wide variety of organisms including mice. To obtain genetically modified embryos or mice by this method, Cas9 mRNA and sgRNA are usually introduced into zygotes by microinjection or electroporation. However, most mutants generated with this method are genetically mosaic, composed of several types of cells carrying different mutations, which complicates phenotype analysis in founder embryos or mice. To simplify the ...
83 CitationsSource
#1Fuminori Tanihara (University of Tokushima)H-Index: 10
#2Tatsuya Takemoto (University of Tokushima)H-Index: 14
Last. Takeshige Otoi (University of Tokushima)H-Index: 31
view all 19 authors...
Genetically modified pigs for biomedical applications have been mainly generated using the somatic cell nuclear transfer technique; however, this approach requires complex micromanipulation techniques and sometimes increases the risks of both prenatal and postnatal death by faulty epigenetic reprogramming of a donor somatic cell nucleus. As a result, the production of genetically modified pigs has not been widely applied. We provide a simple method for CRISPR (clustered regularly interspaced sho...
35 CitationsSource
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