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Jan Philipp Schwarzhans
Bielefeld University
7Publications
5H-index
94Citations
Publications 7
Newest
#1Jan Philipp Schwarzhans (Bielefeld University)H-Index: 5
#2Tobias Luttermann (Bielefeld University)H-Index: 4
Last.Karl Friehs (Bielefeld University)H-Index: 22
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Abstract The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of recombinant proteins, frequently outperforming other heterologous hosts. Already, a sizeable amount of systems biology knowledge has been acquired for this non-conventional yeast. By applying various omics-technologies, productivity features have been thoroughly analyzed and optimized via genetic engineering. However, challenging clonal variability, limited vector repertoire and insufficient g...
28 CitationsSource
#1Jan Philipp Schwarzhans (Bielefeld University)H-Index: 5
#2Tobias Luttermann (Bielefeld University)H-Index: 4
Last.Karl Friehs (Bielefeld University)H-Index: 22
view all 8 authors...
Pichia pastoris is a non-conventional methylotrophic yeast that is widely used for recombinant protein production, typically by stably integrating the target gene into the genome as part of an expression cassette. However, the comparatively high clonal variability associated with this approach usually necessitates a time intense screening step in order to find strains with the desired productivity. Some of the factors causing this clonal variability can be overcome using episomal vectors contain...
6 CitationsSource
The non-conventional methylotrophic yeast *Pichia pastoris* has become a firmly established host for recombinant protein production in both the industry and academia. High product titers, an efficient secretory machinery and the ability to express complex proteins from bacterial to human origin have given *P. pastoris* an advantage over many other host systems. In recent years, its aptitude for foreign gene expression has also been applied in a rising number of metabolic engineering studies. How...
#2Daniel WibbergH-Index: 21
Last.Karl FriehsH-Index: 22
view all 6 authors...
The non-conventional yeast Pichia pastoris is a popular host for recombinant protein production in scientific research and industry. Typically, the expression cassette is integrated into the genome via homologous recombination. Due to unknown integration events, a large clonal variability is often encountered consisting of clones with different productivities as well as aberrant morphological or growth characteristics. In this study, we analysed several clones with abnormal colony morphology and...
19 CitationsSource
#1Jan Philipp Schwarzhans (Bielefeld University)H-Index: 5
#2Daniel Wibberg (Bielefeld University)H-Index: 21
Last.Karl Friehs (Bielefeld University)H-Index: 22
view all 6 authors...
Background The classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although this approach is largely successful, it frequently delivers clones with unpredicted production characteristics and a work-intense screening process is required to find the strain with desired productivity.
27 CitationsSource
#2Daniel WibbergH-Index: 21
Last.Karl FriehsH-Index: 22
view all 6 authors...
Source
#1Jan Philipp Schwarzhans (Bielefeld University)H-Index: 5
#2Dominik Cholewa (Bielefeld University)H-Index: 3
Last.Erwin Flaschel (Bielefeld University)H-Index: 22
view all 7 authors...
Usually, the fatty acid (FA) composition of lipids from microalgae is determined using samples taken at a single time point only, often without considering the medium composition and cultivation conditions. Therefore, the results only represent the FA composition of cells in a certain growth phase. Furthermore, they may misrepresent the capability of the organism to produce certain FA or lipid mixtures. In this study, 22 FA were analysed quantitatively during the cultivation of Euglena gracilis ...
14 CitationsSource
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