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Gautier Robin
University of Queensland
25Publications
10H-index
447Citations
Publications 25
Newest
#2Gautier RobinH-Index: 10
Last.Pierre MartineauH-Index: 27
view all 4 authors...
Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab')2), and to target in a single step the six complementarity-determining regions-or any other part-...
1 CitationsSource
#1Gautier RobinH-Index: 10
#2Yoshiteru Sato (UDS: University of Strasbourg)H-Index: 9
Last.Pierre MartineauH-Index: 27
view all 6 authors...
1 Institut de Recherche en Cancerologie de Montpellier, Montpellier F-34298, France 2 INSERM U896, Montpellier F-34298, France 3 Universite Montpellier 1, Montpellier F-34298, France 4 Institut Regional du Cancer Montpellier, Montpellier F-34298, France 5 Integrated Structural Biology Department, Institute of Genetics and Molecular and Cellular Biology, INSERM U964/CNRS UMR 7104/Universite de Strasbourg, Illkirch F-67404, France 6 Ecole Superieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/...
Source
#1Gautier RobinH-Index: 10
#2Yoshiteru Sato (UDS: University of Strasbourg)H-Index: 9
Last.Pierre MartineauH-Index: 27
view all 6 authors...
Abstract Antibody molecules are able to recognize any antigen with high affinity and specificity. To get insight into the molecular diversity at the source of this functional diversity, we compiled and analyzed a non-redundant aligned collection of 227 structures of antibody–antigen complexes. Free energy of binding of all the residue side chains was quantified by computational alanine scanning, allowing the first large-scale quantitative description of antibody paratopes. This demonstrated that...
31 CitationsSource
#1Kai-En Chen (UQ: University of Queensland)H-Index: 7
#2Ayanthi A. Richards (UQ: University of Queensland)H-Index: 13
Last.Jennifer L. Martin (UQ: University of Queensland)H-Index: 47
view all 12 authors...
The caspase recruitment domain (CARD) is present in death-domain superfamily proteins involved in inflammation and apoptosis. BinCARD is named for its ability to interact with Bcl10 and inhibit downstream signalling. Human BinCARD is expressed as two isoforms that encode the same N-terminal CARD region but which differ considerably in their C-termini. Both isoforms are expressed in immune cells, although BinCARD-2 is much more highly expressed. Crystals of the CARD fold common to both had low sy...
6 CitationsSource
#1Gautier RobinH-Index: 10
#2Pierre Martineau (French Institute of Health and Medical Research)H-Index: 27
Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or few frameworks. Diversity is introduced in the third hypervariable loops using degenerate synthetic oligonucleotides and PCR assembly. Because all the antibody fragments isolated from the library will share their framework sequence, their stability and physical...
7 CitationsSource
#1Adeline GouletH-Index: 6
Last.Patrick BronH-Index: 23
view all 12 authors...
Abstract The SPP1 siphophage uses its long non-contractile tail and tail tip to recognize and infect the Gram-positive bacterium Bacillus subtilis. The tail-end cap and its attached tip are the critical components for host recognition and opening of the tail tube for genome exit. In the present work, we determined the cryo-electron microscopic (cryo-EM) structure of a complex formed by the cap protein gp19.1 (Dit) and the N terminus of the downstream protein of gp19.1 in the SPP1 genome, gp211–5...
35 CitationsSource
#1David VeeslerH-Index: 24
#2Gautier RobinH-Index: 10
Last.Christian CambillauH-Index: 71
view all 8 authors...
Siphophage SPP1 infects the Gram-positive bacterium Bacillus subtilis using its long non-contractile tail and tail-tip. Electron microscopy (EM) previously allowed a low resolution assignment of most orf products belonging to these regions. We report here the structure of the SPP1 distal tail protein (Dit, gp19.1). The combination of x-ray crystallography, EM, and light scattering established that Dit is a back-to-back dimer of hexamers. However, Dit fitting in the virion EM maps was only possib...
52 CitationsSource
#1David VeeslerH-Index: 24
#2Gautier RobinH-Index: 10
Last.Christian CambillauH-Index: 71
view all 8 authors...
Source
Dec 1, 2009 in e-Science (International Conference on e-Science)
#1Charles Brooking (UQ: University of Queensland)H-Index: 4
#2Stephen R. Shouldice (UQ: University of Queensland)H-Index: 7
Last.Jane Hunter (UQ: University of Queensland)H-Index: 29
view all 6 authors...
This paper describes the set of eResearch services developed by the eResearch Lab within the University of Queensland (UQ) for the Structural Genomics (SG) Group at UQ. The aim of these services is to enable collaborative teams of protein crystallographers in the SG group to track their experiments and to manage the plethora and diversity of data that they generate through distributed high-throughput approaches and complex scientific workflows. More specifically we describe: the secure Web-based...
Source
#1Gordon J. King (UQ: University of Queensland)H-Index: 15
#2Kai-En Chen (UQ: University of Queensland)H-Index: 7
Last.Jennifer L. Martin (UQ: University of Queensland)H-Index: 47
view all 9 authors...
Background Protein crystallisation screening involves the parallel testing of large numbers of candidate conditions with the aim of identifying conditions suitable as a starting point for the production of diffraction quality crystals. Generally, condition screening is performed in 96-well plates. While previous studies have examined the effects of protein construct, protein purity, or crystallisation condition ingredients on protein crystallisation, few have examined the effect of the crystalli...
10 CitationsSource
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