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Michel W. F. Nielen
Wageningen University and Research Centre
Analytical chemistryMass spectrometryChemistryTandem mass spectrometryChromatography
214Publications
45H-index
6,170Citations
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Publications 218
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#1Marco Blokland (WUR: Wageningen University and Research Centre)H-Index: 18
#2Arjen Gerssen (WUR: Wageningen University and Research Centre)H-Index: 12
Last. Michel W. F. Nielen (WUR: Wageningen University and Research Centre)H-Index: 45
view all 5 authors...
In food analysis, a trend towards on-site testing of quality and safety parameters is emerging. So far, on-site testing has been mainly explored by miniaturized optical spectroscopy and ligand-binding assay approaches such as lateral flow immunoassays and biosensors. However, for the analysis of multiple parameters at regulatory levels, mass spectrometry (MS) is the method of choice in food testing laboratories. Thanks to recent developments in ambient ionization and upcoming miniaturization of ...
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Identification and confirmation of known as well as unknown (bio)chemical entities in ambient mass spectrometry (MS) and MS imaging (MSI) mostly involve accurate mass determination, often in combination with MS/MS or MSn work flows. To further improve structural assignment, additional molecular information is required. Here we present an ambient hydrogen/deuterium exchange (HDX) laser ablation electrospray ionization (LAESI) MS method in which, apart from the accurate mass and MS/MS data, the nu...
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#1Milou J.C. Santbergen (WUR: Wageningen University and Research Centre)
Last. Michel W. F. Nielen (WUR: Wageningen University and Research Centre)H-Index: 45
view all 5 authors...
In oral bioavailability studies, evaluation of the absorption and transport of drugs and food components across the intestinal barrier is crucial. Advances in the field of organ-on-a-chip technology have resulted in a dynamic gut-on-a-chip model that better mimics the in vivo microenvironment of the intestine. Despite a few recent integration attempts, ensuring a biologically relevant microenvironment while coupling with a fully online detection system still represents a major challenge. Herein,...
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(1) Background: The lack of globally standardized allergen labeling legislation necessitates consumer-focused multiplexed testing devices. These should be easy to operate, fast, sensitive and robust. (2) Methods: Herein, we describe the development of three different formats for multiplexed food allergen detection, namely active and passive flow-through assays, and lateral flow immunoassays with different test line configurations. (3) Results: The fastest assay time was 1 min, whereas even the s...
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#1Arjen Lommen (WUR: Wageningen University and Research Centre)H-Index: 32
#2Abdurzag Elaradi (Qatar Airways)
Last. Costas Georgakopoulos (Qatar Airways)H-Index: 19
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Rationale: Retroactive analysis of previously tested urine samples has become an important sports anti-doping tool. Retroactive reprocessing of old data files acquired from a generic screening procedure can reveal detection of initially unknown substances, like illegal drugs and newly identified metabolites. Methods: To be able to efficiently search through hundreds to thousands of liquid chromatography high-resolution full-scan Orbitrap mass spectrometry data files of anti-doping samples, a com...
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#1Pim de HaanH-Index: 2
#2Milou J.C. Santbergen (WUR: Wageningen University and Research Centre)
Last. Elisabeth VerpoorteH-Index: 44
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This work describes our efforts to date to realize a miniaturized gastrointestinal (GI) tract total analysis system (gut-μTAS) for nutrients or drugs upon ingestion. A multi-module flow system is being developed that seeks to combine all processes of the GI tract, including enzymatic digestion, absorption, and metabolism together with online mass spectrometry analysis. When exposing cells to digested materials (chyme), we found that the cells tolerate up to ~15% of chyme in the cellular matrix. ...
#1Milou J.C. Santbergen (WUR: Wageningen University and Research Centre)
Last. Michel W. F. Nielen (WUR: Wageningen University and Research Centre)H-Index: 45
view all 4 authors...
Abstract Organ-on-a-chip technology is used to study biological processes that involve multiple cell types and temporal changes like, homeostasis, metabolism and responses to chemical triggers. Main benefits of organ-on-a-chip systems include: improved mimicking of the in vivo situation, easy manipulation of the microenvironment and low reagent consumption. Exploiting the unique dynamic aspects of organ-on-a-chip technology, such as liquid flow, automated online measurement of parameters by sens...
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#1Fred A. M. G. van Geenen (WUR: Wageningen University and Research Centre)H-Index: 2
#2Maurice C. R. Franssen (WUR: Wageningen University and Research Centre)H-Index: 19
Last. Michel W. F. Nielen (WUR: Wageningen University and Research Centre)H-Index: 45
view all 7 authors...
In drug discovery, it is important to identify phase I metabolic modifications as early as possible to screen for inactivation of drugs and/or activation of prodrugs. As the major class of reactions in phase I metabolism is oxidation reactions, oxidation of drugs with TiO2 photocatalysis can be used as a simple non-biological method to initially eliminate (pro)drug candidates with an undesired phase I oxidation metabolism. Analysis of reaction products is commonly achieved with mass spectrometry...
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#1Nevena Klisara (NTU: Nanyang Technological University)H-Index: 1
#2Jeroen Peters (WUR: Wageningen University and Research Centre)H-Index: 10
Last. Bo Liedberg (NTU: Nanyang Technological University)H-Index: 52
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Abstract The extremely toxic botulinum neurotoxin poses a threat for health and food safety, requiring rapid and easy-to-use detection platforms. To meet these requirements, we have explored a novel functional assay format for detection of botulinum neurotoxin serotype A light chain (BoNT/A LC) in complex matrices. The proposed assay utilizes a synthetic peptide designed to mimic the SNAP-25 protein (synaptosomal-associated protein 25) as substrate bound to a superparamagnetic bead and a fluores...
3 CitationsSource
#1Wadha Abushareeda (Qatar Airways)H-Index: 4
#2Marc Tienstra (WUR: Wageningen University and Research Centre)H-Index: 3
Last. Costas Georgakopoulos (Qatar Airways)H-Index: 19
view all 10 authors...
Rationale: The World Anti-Doping Agency (WADA) encourages drug-testing laboratories to develop screening methods that can detect as many doping substances as possible in urine. The use of full-scan high-resolution acquisition (FS/HR) with gas chromatography/mass spectrometry (GC/MS) for the detection of known and unknown trimethylsilyl (TMS) derivatives of anabolic-androgenic steroids (AAS) provides anti-doping testing bodies with a new analytical tool. Methods: The AAS were extracted from urine...
3 CitationsSource
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