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Ah Ram Lee
Konkuk University
Drug resistanceVirologyHepatitis B virusHBxBiology
13Publications
7H-index
110Citations
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Publications 13
Newest
#1Soree Park (Konkuk University)H-Index: 8
#2Eun-Sook Park (Konkuk University)H-Index: 11
Last. Jeong-Hoon Lee (SNU: Seoul National University)H-Index: 24
view all 12 authors...
BACKGROUND & AIM: Since polymerase and surface genes overlap in hepatitis B virus (HBV), an antiviral-induced mutation in the polymerase gene may alter the surface antigenicity in patients with chronic hepatitis B (CHB), but this possibility has not been clearly confirmed. This study aimed to determine the drug susceptibility and surface antigenicity of the patient-derived mutants. PATIENTS AND METHODS: Full-length HBV genomes isolated from four entecavir-resistant CHB patients were cloned and s...
1 CitationsSource
#1Yong Kwang ParkH-Index: 11
#1Yong Kwang Park (Center for Infectious Disease Research and Policy)H-Index: 1
Last. Byeong-Sun Choi (Center for Infectious Disease Research and Policy)H-Index: 2
view all 7 authors...
BACKGROUND AND AIM: Interferon-stimulated gene 20 (ISG20) is an interferon-inducible exonuclease that inhibits the replication of several RNA viruses. In patients with chronic hepatitis B, ISG20 expression is related to the interferon-alpha treatment response. However, the molecular mechanism of ISG20-mediated anti-hepatitis B virus (HBV) activity is unclear. METHODS: We have investigated the effect of ISG20 on antiviral activity to address that. The life cycle of HBV was analyzed by the ectopic...
Source
#1Soree Park (Konkuk University)H-Index: 8
#2Yea Na Ha (Konkuk University)H-Index: 3
Last. Jae Jin Shin (Konkuk University)
view all 12 authors...
Hepatitis B virus (HBV) infection is a major factor in the development of various liver diseases such as hepatocellular carcinoma (HCC). Among HBV encoded proteins, HBV X protein (HBx) is known to play a key role in the development of HCC. Hepatocyte nuclear factor 4α (HNF4α) is a nuclear transcription factor which is critical for hepatocyte differentiation. However, the expression level as well as its regulatory mechanism in HBV infection have yet to be clarified. Here, we observed the suppress...
Source
#1Eun-Sook Park (Konkuk University)H-Index: 11
#2Ah Ram Lee (Konkuk University)H-Index: 7
Last. Kyun-Hwan Kim (Konkuk University)H-Index: 22
view all 26 authors...
Background & Aims Tenofovir disoproxil fumarate (TDF) is one the most potent nucleot(s)ide analogues for treating chronic hepatitis B virus (HBV) infection. Phenotypic resistance caused by genotypic resistance to TDF has not been reported. This study aimed to characterize HBV mutations that confer tenofovir resistance. Methods Two patients with viral breakthrough during treatment with TDF-containing regimens were prospectively enrolled. The gene encoding HBV reverse transcriptase was sequenced. ...
5 CitationsSource
#1Eun-Sook Park (Konkuk University)H-Index: 11
#2Young-Ho Byun (Yonsei University)H-Index: 14
Last. Kyun-Hwan Kim (Konkuk University)H-Index: 22
view all 25 authors...
: The multifunctional influenza virus protein PB1-F2 plays several roles in deregulation of host innate immune responses and is a known immunopathology enhancer of the 1918 influenza pandemic. Here, we show that the 1918 PB1-F2 protein not only interferes with the mitochondria-dependent pathway of type I interferon (IFN) signaling, but also acquired a novel IFN antagonist function by targeting the DEAD-box helicase DDX3, a key downstream mediator in antiviral interferon signaling, toward proteas...
3 CitationsSource
#1Doo Hyun Kim (Konkuk University)H-Index: 9
#2Eun-Sook Park (Konkuk University)H-Index: 11
Last. Kyun-Hwan Kim (Konkuk University)H-Index: 22
view all 17 authors...
Cytokines are involved in early host defense against pathogen infections. In particular, tumor necrosis factor (TNF) and interferon-gamma (IFN-γ) have critical functions in non-cytopathic elimination of hepatitis B virus (HBV) in hepatocytes. However, the molecular mechanisms and mediator molecules are largely unknown. Here we show that interleukin-32 (IL-32) is induced by TNF and IFN-γ in hepatocytes, and inhibits the replication of HBV by acting intracellularly to suppress HBV transcription an...
3 CitationsSource
#1Ah Ram Lee (Konkuk University)H-Index: 7
#2Keo Heun Lim (Konkuk University)H-Index: 1
Last. Kyun-Hwan Kim (Konkuk University)H-Index: 22
view all 15 authors...
Hepatitis B virus (HBV) infection is a leading cause of liver diseases; however, the host factors which facilitate the replication and persistence of HBV are largely unidentified. Cellular FLICE inhibitory protein (c-FLIP) is a typical antiapoptotic protein. In many cases of liver diseases, the expression level of c-FLIP is altered, which affects the fate of hepatocytes. We previously found that c-FLIP and its cleaved form interact with HBV X protein (HBx), which is essential for HBV replication...
1 CitationsSource
#1Doo Hyun KimH-Index: 9
#2Hong Seok KangH-Index: 8
Last. Kyun-Hwan Kim (Konkuk University)H-Index: 22
view all 12 authors...
Background/Aims: Direct sequencing is the gold standard for the detection of drug-resistance mutations in hepatitis B virus (HBV); however, this procedure is time-consuming, labor-intensive, and difficult to adapt to high-throughput screening. In this study, we aimed to develop a dendron-modified DNA microarray for the detection of genotypic resistance mutations and evaluate its efficiency. Methods: The specificity, sensitivity, and selectivity of dendron-modified slides for the detection of rep...
1 CitationsSource
#1Keo Heun Lim (Konkuk University)H-Index: 1
#2Eun-Sook Park (Konkuk University)H-Index: 11
Last. Kyun-Hwan Kim (Konkuk University)H-Index: 22
view all 23 authors...
Objective Interferons (IFNs) mediate direct antiviral activity. They play a crucial role in the early host immune response against viral infections. However, IFN therapy for HBV infection is less effective than for other viral infections. Design We explored the cellular targets of HBV in response to IFNs using proteome-wide screening. Results Using LC-MS/MS, we identified proteins downregulated and upregulated by IFN treatment in HBV X protein (HBx)-stable and control cells. We found several IFN...
18 CitationsSource
#1Gu Choul Shin (Konkuk University)H-Index: 12
#2Hong Seok Kang (Konkuk University)H-Index: 8
Last. Kyun-Hwan Kim (Konkuk University)H-Index: 22
view all 4 authors...
ABSTRACTDeath receptors of TNFSF10/TRAIL (tumor necrosis factor superfamily member 10) contribute to immune surveillance against virus-infected or transformed cells by promoting apoptosis. Many viruses evade antiviral immunity by modulating TNFSF10 receptor signaling, leading to persistent infection. Here, we report that hepatitis B virus (HBV) X protein (HBx) restricts TNFSF10 receptor signaling via macroautophagy/autophagy-mediated degradation of TNFRSF10B/DR5, a TNFSF10 death receptor, and th...
15 CitationsSource
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