Match!
Almudena Perona
European University of Madrid
Molecular biologyChemistryFtsZNucleotideBiochemistry
16Publications
7H-index
147Citations
What is this?
Publications 15
Newest
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Almudena Perona (UEM: European University of Madrid)H-Index: 7
Last. Pedro A. Sánchez-Murcia (University of Vienna)H-Index: 8
view all 6 authors...
Insight into the catalytic mechanism of Lactobacillus leichmannii nucleoside 2′-deoxyribosyltransferase (LlNDT) has been gained by calculating a quantum mechanics–molecular mechanics (QM/MM) free-energy landscape of the reaction within the enzyme active site. Our results support an oxocarbenium species as the reaction intermediate and thus an SN1 reaction mechanism in this family of bacterial enzymes. Our mechanistic proposal is validated by comparing experimental kinetic data on the impact of t...
Source
#1Cristina Benito-Muñoz (UAM: Autonomous University of Madrid)H-Index: 1
#2Almudena PeronaH-Index: 7
Last. Beatriz López-Corcuera (CSIC: Spanish National Research Council)H-Index: 23
view all 7 authors...
Neurotransmitter removal from glycine-mediated synapses relies on two sodium-driven high-affinity plasma membrane glycine transporters (GlyTs) that control neurotransmitter availability. Mostly glial GlyT1 is the main regulator of glycine synaptic levels, whereas neuronal GlyT2 promotes the recycling of synaptic glycine and supplies neurotransmitter for presynaptic vesicle refilling. The GlyTs differ in sodium:glycine symport stoichiometry, showing GlyT1 a 2:1 and GlyT2 a 3:1 sodium:glycine coup...
2 CitationsSource
#2Salvador DueñasH-Index: 2
Last. Noemí Sánchez-CamposH-Index: 1
view all 19 authors...
// Dalia Millan-Gomez 1 , Salvador Duenas 1 , Patricia L.A. Munoz 2 , Tanya Camacho-Villegas 3 , Carolina Elosua 4 , Olivia Cabanillas-Bernal 1 , Teresa Escalante 5 , Almudena Perona 6 , David Abia 6 , Florian Drescher 1 , Pierrick G.J. Fournier 1 , Marco A. Ramos 2 , Rosa E. Mares 2 , Jorge Paniagua-Solis 4 , Teresa Mata-Gonzalez 7 , Jorge Gonzalez-Canudas 7 , Robert M. Hoffman 8, 9 , Alexei Licea-Navarro 1 and Noemi Sanchez-Campos 1 1 Departamento de Innovacion Biomedica, Centro de Investigaci...
2 CitationsSource
#1Jon Del ArcoH-Index: 4
Last. Jesús Fernández-LucasH-Index: 12
view all 8 authors...
The enzymatic synthesis of nucleotides offers several advantages over traditional multistep chemical methods, such as stereoselectivity, regioselectivity, enantioselectivity, simple downstream processing, and the use of mild reaction conditions. However, in order to scale up these bioprocesses, several drawbacks, such as the low enzyme stability and recycling, must be considered. Enzyme immobilization may overcome these cost-related problems by enhancing protein stability and facilitating the se...
5 CitationsSource
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Javier Acosta (UEM: European University of Madrid)H-Index: 2
Last. Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 7 authors...
The use of enzymes as biocatalysts applied to synthesis of modified nucleoside-5'-monophosphates (NMPs) is an interesting alternative to traditional multistep chemical methods which offers several advantages, such as stereo, regio and enantioselectivity, simple downstream processing, and mild reaction conditions. Herein we report the recombinant expression, production and purification of uracil phosphoribosyltransferase from Thermus themophilus HB8 (TtUPRT). The structure of TtUPRT has been dete...
5 CitationsSource
#1J. Del Arco (UEM: European University of Madrid)H-Index: 1
#2J. Cejudo-Sanches (UEM: European University of Madrid)H-Index: 2
Last. Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 7 authors...
Abstract Traditionally, enzymatic synthesis of nucleoside-5′-monophosphates (5′-NMPs) using low water-soluble purine bases has been described as less efficient due to their low solubility in aqueous media. The use of enzymes from extremophiles, such as thermophiles or alkaliphiles, offers the potential to increase solubilisation of these bases by employing high temperatures or alkaline pH. This study describes the cloning, expression and purification of hypoxanthine-guanine-xanthine phosphoribos...
7 CitationsSource
#1Irene Bravo-Alonso (CSIC: Spanish National Research Council)H-Index: 2
#2Rosa Navarrete (CSIC: Spanish National Research Council)H-Index: 11
Last. Pilar Rodríguez-Pombo (CSIC: Spanish National Research Council)H-Index: 17
view all 16 authors...
The rapid analysis of genomic data is providing effective mutational confirmation in patients with clinical and biochemical hallmarks of a specific disease. This is the case for nonketotic hyperglycinemia (NKH), a Mendelian disorder causing seizures in neonates and early-infants, primarily due to mutations in the GLDC gene. However, understanding the impact of missense variants identified in this gene is a major challenge for the application of genomics into clinical practice. Herein, a comprehe...
2 CitationsSource
#1Aldo Borroto (CSIC: Spanish National Research Council)H-Index: 13
#2Diana Reyes-Garau (CSIC: Spanish National Research Council)H-Index: 2
Last. Balbino Alarcón (CSIC: Spanish National Research Council)H-Index: 41
view all 32 authors...
Modulating T cell activation is critical for treating autoimmune diseases but requires avoiding concomitant opportunistic infections. Antigen binding to the T cell receptor (TCR) triggers the recruitment of the cytosolic adaptor protein Nck to a proline-rich sequence in the cytoplasmic tail of the TCR’s CD3e subunit. Through virtual screening and using combinatorial chemistry, we have generated an orally available, low–molecular weight inhibitor of the TCR-Nck interaction that selectively inhibi...
16 CitationsSource
#1Sonia HuecasH-Index: 15
#2Filipa MarceloH-Index: 16
Last. José Manuel AndreuH-Index: 48
view all 8 authors...
FtsZ is the organizer of cell division in most bacteria and a target in the quest for new antibiotics. FtsZ is a tubulin-like GTPase, in which the active site is completed at the interface with the next subunit in an assembled FtsZ filament. Fluorescent mant-GTP has been extensively used for competitive binding studies of nucleotide analogs and synthetic GTP-replacing inhibitors possessing antibacterial activity. However, its mode of binding and whether the mant tag interferes with FtsZ assembly...
6 CitationsSource
Last. Eduardo Andrés-LeónH-Index: 1
view all 24 authors...
S1-5 “Bioinformatica con N v1.0”: a collaborative project of young Spanish scientists to write a complete book about Bioinformatics Alvaro Sebastian , Alberto Pascual-Garcia , Federico Abascal , Jacobo Aguirre , Eduardo AndresLeon , Djordje Bajic , Davide Bau , Juan A. Bueren-Calabuig , Alvaro Cortes-Cabrera , Ivan Dotu , Jose M. Fernandez , Helena G. Dos Santos , Beatriz Garcia-Jimenez , Raul Guantes , Iker Irisarri , Natalia Jimenez-Lozano , Javier Klett , Raul Mendez , Antonio Morreale , Almu...
12