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Derek S. Damron
Cleveland Clinic
Protein kinase CMyocyteAnesthesiaMedicineIntracellular
62Publications
23H-index
1,629Citations
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Publications 62
Newest
#1Peter J. WickleyH-Index: 7
#2Ryo YugeH-Index: 2
Last. Derek S. DamronH-Index: 23
view all 5 authors...
Background: Myocardial protection by anesthetics is known to involve activation of protein kinase C epsilon (PKCe). A key step in the activation process is autophosphorylation of the enzyme at serine 729. This study's objectives were to identify the extent to which propofol interacts with PKCe and to identify the molecular mechanism(s) of interaction. Methods: Immunoblot analysis of recombinant PKCe was used to assess autophosphorylation of PKCe at serine 729 before and after exposure to propofo...
4 CitationsSource
#1Daniel Jane-wit (Cleveland Clinic)H-Index: 6
#2Cengiz Z. Altuntas (Cleveland Clinic)H-Index: 11
Last. Vincent K. Tuohy (Cleveland Clinic)H-Index: 30
view all 12 authors...
Background— Antibodies to the β1-adrenergic receptor (β1AR) are detected in a substantial number of patients with idiopathic dilated cardiomyopathy (DCM). The mechanism whereby these autoantibodies exert their pathogenic effect is unknown. Here, we define a causal mechanism whereby β1AR-specific autoantibodies mediate noninflammatory cardiomyocyte cell death during murine DCM. Methods and Results— We used the β1AR protein as an immunogen in SWXJ mice and generated a polyclonal battery of autoant...
72 CitationsSource
#1Peter J. WickleyH-Index: 7
#2Toshiya ShigaH-Index: 12
Last. Derek S. DamronH-Index: 23
view all 4 authors...
Background: The authors' objective was to identify the role of the Na + -Ca2 + exchanger (NCX) in mediating the contractile dysfunction observed in diabetic cardiomyocytes before and after exposure to propofol. Methods: Freshly isolated ventricular myocytes were obtained from normal and diabetic rat hearts. Intracellular concentration of Ca 2+ and cell shortening were simultaneously measured in electrically stimulated, ventricular myocytes using fura-2 and video-edge detection, respectively. Pos...
15 CitationsSource
#1Peter J. WickleyH-Index: 7
#2Xueqin DingH-Index: 7
Last. Derek S. DamronH-Index: 23
view all 4 authors...
Background: Myocardial protection by anesthetics is known to involve activation of protein kinase C (PKC). The authors’ objective was to identify the PKC isoforms activated by propofol in rat ventricular myocytes. They also assessed the intracellular location of individual PKC isoforms before and after treatment with propofol. Methods: Freshly isolated ventricular myocytes were obtained from adult rat hearts. Immunoblot analysis of cardiomyocyte subcellular fractions was used to assess transloca...
39 CitationsSource
#1Peter J. WickleyH-Index: 7
#2Toshiya ShigaH-Index: 12
Last. Derek S. DamronH-Index: 23
view all 4 authors...
Background: The authors' objective was to assess the role of protein kinase C (PKC) and nitric oxide synthase (NOS) in mediating the effects of propofol on diabetic cardiomyocyte contractility, intracellular free Ca 2+ concentration ([Ca 2+ ] i ), and myofilament Ca 2+ sensitivity. Methods: Freshly isolated ventricular myocytes were obtained from normal and diabetic rat hearts. [Ca 2+ ] i and cell shortening were simultaneously measured in electrically stimulated, ventricular myocytes using fura...
22 CitationsSource
#1Noriaki Kanaya (Sapporo Medical University)H-Index: 7
#2Paul A. Murray (Cleveland Clinic)H-Index: 25
Last. Derek S. Damron (Cleveland Clinic)H-Index: 23
view all 3 authors...
Purpose Our objective was to determine whether an L-type Ca2+ channel modulation could alter myocardial depression induced by midazolam or diazepam in adult rat ventricular myocytes.
7 CitationsSource
#1Noriaki KanayaH-Index: 7
#2Brad GableH-Index: 3
Last. Derek S. DamronH-Index: 23
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Background: The rationale for this study is that the depressant effect of propofol on cardiac function in vitro is highly variable but may be explained by differences in the temperature and stimulation frequency used for the study. Both temperature and stimulation frequency are known to modulate cellular mechanisms that regulate intracellular free Ca 2+ concentration ([Ca 2+ ] i ) and myofilament Ca 2+ sensitivity in cardiac muscle. The authors hypothesized that temperature and stimulation frequ...
12 CitationsSource
#1Brad GableH-Index: 3
#2Toshiya ShigaH-Index: 12
Last. Derek S. DamronH-Index: 23
view all 4 authors...
Background: The objective of this study was to identify the extent to which propofol alters intracellular free Ca 2+ concentration ([Ca 2+ ] i ), myofilament Ca 2+ sensitivity, and contraction of individual cardiomyocytes during activation of α 1a adrenoreceptors and to determine the cellular mechanism of action. Methods: Freshly isolated ventricular myocytes were obtained from adult rat hearts. Myocyte shortening and [Ca 2+ ] i were simultaneously monitored in individual cardiomyocytes exposed ...
11 CitationsSource
#1Toshiya ShigaH-Index: 12
#2Sandro L. YongH-Index: 11
Last. Derek S. DamronH-Index: 23
view all 5 authors...
Background: Droperidol has recently been associated with cardiac arrhythmias and sudden cardiac death. Changes in action potential duration seem to be the cause of the arrhythmic behavior, which can lead to alterations in intracellular free Ca 2+ concentration ([Ca 2+ ] i ). Because [Ca 2+ ] i and myofilament Ca 2+ sensitivity are key regulators of myocardial contractility, the authors' objective was to identify whether droperidol alters [Ca 2+ ] i or myofilament Ca 2+ sensitivity in rat ventric...
4 CitationsSource
#1Xueqin DingH-Index: 7
#2Derek S. DamronH-Index: 23
Last. Paul A. MurrayH-Index: 25
view all 3 authors...
Background: The authors investigated the extent and cellular mechanisms by which the intravenous anesthetic ketamine alters acetylcholine-induced contraction in pulmonary veins (PVs). They tested the hypothesis that ketamine inhibits acetylcholine contraction in PVs. Methods: Canine PV rings with endothelium (E+) and without endothelium (E-) were isolated for measurement of isometric tension. The effects of ketamine (10 -5 M∼10 -3 M) on acetylcholine contraction were assessed in E+ and E- rings....
5 CitationsSource
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