Ivan Sabolić
Harvard University
Publications 137
Abstract Metallothioneins (MTs) exhibit binding affinity for several essential and toxic trace elements. Previous studies in rodents indicated sex differences in the hepatic and renal expression of MTs and concentrations of various elements. The mechanism responsible for these differences has not been resolved. Here, in the liver and kidney tissues of sham-operated and gonadectomized male and female rats we determined the expression of MT1 and MT2 (MT1&2) mRNA by RT-PCR, abundance of MT1&2 prote...
1 CitationsSource
#2Josip Madunić (University of Zagreb)H-Index: 5
Last.Ivan SabolićH-Index: 34
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#1Yiling Fu (UCSD: University of California, San Diego)H-Index: 3
#2Davorka BreljakH-Index: 13
Last.Volker Vallon (UCSD: University of California, San Diego)H-Index: 60
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The SGLT2 inhibitor empagliflozin (plasma protein binding ~88%) may reach its target in the brush border of the early proximal tubule by glomerular filtration and tubular secretion. Here we determined whether empagliflozin is secreted by renal tubules, and whether genetic knockout of the basolateral organic anion transporter Oat3 (-/-) affects its tubular secretion or glucosuric effect. Renal clearance studies in wild-type (WT) mice showed that tubular secretion accounted for 50-70% of empaglifl...
2 CitationsSource
#2Dean KaraicaH-Index: 4
Last.Davorka BreljakH-Index: 13
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#1Ivan SabolićH-Index: 34
#2Mario SkaricaH-Index: 14
Last.Nikola Ljubešić (Croatian Academy of Sciences and Arts)H-Index: 1
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Abstract Rodent kidneys exhibit three isoforms of metallothioneins (MTs), MT1, MT2 and MT3, with poorly characterized localization along the nephron. Here we studied in adult male Wistar rats the renal expression of MTs mRNA by end-point RT-PCR and MT proteins by immunochemical methods The expression pattern of MT1 mRNA was cortex (CO) > outer stripe (OS) = inner stripe (IS) = inner medulla (IM), of MT2 mRNA was IM > CO > IS = OS, and of MT3 mRNA was IM > CO = OS = IM. MT1/2-antibody stained wit...
4 CitationsSource
#1Madhuri S. Salker (University of Tübingen)H-Index: 17
#2Yogesh Singh (University of Tübingen)H-Index: 16
Last.Florian Lang (University of Tübingen)H-Index: 105
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Embryo implantation requires a hospitable uterine environment. A key metabolic change that occurs during the peri-implantation period, and throughout early pregnancy, is the rise in endometrial glycogen content. Glycogen accumulation requires prior cellular uptake of glucose. Here we show that both human and murine endometrial epithelial cells express the high affinity Na+-coupled glucose carrier SGLT1. Ussing chamber experiments revealed electrogenic glucose transport across the endometrium in ...
9 CitationsSource
The expression and localization of sodium-d-glucose cotransporter SGLT1 (SLC5A1), which is involved in small intestinal glucose absorption and renal glucose reabsorption, is of high biomedical relevance because SGLT1 inhibitors are currently tested for antidiabetic therapy. In human and rat organs, detailed expression profiling of SGLT1/Sglt1 mRNA and immunolocalization of the transporter protein has been performed. Using polyspecific antibodies and preabsorption with antigenic peptide as specif...
9 CitationsSource
Concentrations of macro- and microelements in animal organs indicate the animal health status and represent reference data for animal experiments. Their levels in blood and tissues could be different between sexes, and could be different with and without blood in tissues. To test these hypotheses, in adult female and male rats the concentrations of various elements were measured in whole blood, blood plasma, and tissues from blood-containing (nonperfused) and blood-free liver, kidneys, and brain...
3 CitationsSource
#1Piyush SharmaH-Index: 6
#2Vishal KhairnarH-Index: 7
Last.Karl S. LangH-Index: 35
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Background: Cellular glucose uptake may involve either non-concentrative glucose carriers of the GLUT family or Na+-coupled glucose-carrier SGLT1, whic
7 CitationsSource