Sebastien Verhenne
Katholieke Universiteit Leuven
Sleeping Beauty transposon systemImmunologyVon Willebrand factorADAMTS13Medicine
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Publications 12
#1Sirima Kraisin (Katholieke Universiteit Leuven)H-Index: 1
#2Kimberly Martinod (Katholieke Universiteit Leuven)H-Index: 23
Last. Simon F. De Meyer (Katholieke Universiteit Leuven)H-Index: 24
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BACKGROUND Cerebral malaria (CM) is the most severe complication in malaria. Endothelial activation, cytokine release and vascular obstruction are essential hallmarks of CM. Clinical studies have suggested a link between von Willebrand factor (VWF) and malaria pathology. OBJECTIVES To investigate the contribution of VWF in the pathogenesis of experimental cerebral malaria (ECM). METHODS Both Vwf+/+ and Vwf-/- mice were infected with Plasmodium berghei ANKA (PbANKA) to induce ECM. Alterations of ...
#1Sirima Kraisin (Katholieke Universiteit Leuven)H-Index: 1
#2Sebastien Verhenne (Katholieke Universiteit Leuven)H-Index: 6
Last. Simon F. DeMeyer (Katholieke Universiteit Leuven)H-Index: 22
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BACKGROUND: Malaria-associated acute respiratory distress syndrome (MA-ARDS) is a lethal complication of severe malaria, characterized by marked pulmonary inflammation. Patient studies have suggested a link between von Willebrand factor (VWF) and malaria severity. OBJECTIVES: To investigate the role of VWF in the pathogenesis of experimental MA-ARDS. METHODS: Plasmodium berghei NK65-E (PbNK65) parasites were injected in Vwf+/+ and Vwf-/- mice. Pathological parameters were assessed following infe...
3 CitationsSource
#1Mickael QuivigerH-Index: 3
#2Aristeidis Giannakopoulos (University of Patras)H-Index: 2
Last. Daniel SchermanH-Index: 59
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Abstract Many rare monogenic diseases are treated by protein replacement therapy, in which the missing protein is repetitively administered to the patient. However, in several cases, the missing protein is required at a high and sustained level, which renders protein therapy far from being adequate. As an alternative, a gene therapy treatment ensuring a sustained effectiveness would be particularly valuable. Liver is an optimal organ for the secretion and systemic distribution of a therapeutic t...
1 CitationsSource
#1Irina Portier (Katholieke Universiteit Leuven)H-Index: 2
#2Karen Vanhoorelbeke (Katholieke Universiteit Leuven)H-Index: 38
Last. Simon F. DeMeyer (Katholieke Universiteit Leuven)H-Index: 22
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BACKGROUND: Type 3 von Willebrand disease (VWD) is characterized by complete absence of von Willebrand factor (VWF). Current therapy is limited to treatment with exogenous VWF/FVIII products, which only provide a short-term solution. Gene therapy offers the potential for a long-term treatment for VWD. OBJECTIVES: To develop an integrative Sleeping Beauty (SB) transposon-mediated VWF gene transfer approach in a preclinical mouse model of severe VWD. METHODS: We established a robust platform for s...
6 CitationsSource
Objective—Severe deficiency in the von Willebrand factor–cleaving protease ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13) because of mutations in the ADA...
8 CitationsSource
#1Claudia Tersteeg (Katholieke Universiteit Leuven)H-Index: 13
#2Sebastien Verhenne (Katholieke Universiteit Leuven)H-Index: 6
Last. Karen Vanhoorelbeke (Katholieke Universiteit Leuven)H-Index: 38
view all 7 authors...
A deficiency in ADAMTS13 (A Disintegrin And Metalloprotease with ThromboSpondin type-1 repeats, member 13) is associated with thrombotic thrombocytopenic purpura (TTP). Congenital TTP is caused by a defect in the ADAMTS13 gene resulting in decreased or absent enzyme activity; acquired TTP results from autoantibodies that either inhibit the activity or increase the clearance of ADAMTS13. Despite major progress in recent years in our understanding of the disease, many aspects around the pathophysi...
10 CitationsSource
#1Sebastien Verhenne (Katholieke Universiteit Leuven)H-Index: 6
#2Frederik Denorme (Katholieke Universiteit Leuven)H-Index: 10
Last. Simon F. De Meyer (Katholieke Universiteit Leuven)H-Index: 13
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Abstract Von Willebrand factor (VWF) is a key hemostatic protein synthesized in both endothelial cells and megakaryocytes. Megakaryocyte-derived VWF is stored in α-granules of platelets and is enriched in hyperactive "ultra-large" VWF multimers. To elucidate the specific contribution of platelet VWF in hemostasis and thrombosis, we performed crossed bone marrow transplantations between C57BL/6J and Vwf(-/-) mice to generate chimeric mice. Chimeric mice specifically lacking platelet VWF showed no...
37 CitationsSource
1 Citations
#1Sebastien VerhenneH-Index: 6
#2Sarah LibbrechtH-Index: 5
Last. Simon F. DeMeyerH-Index: 22
view all 7 authors...