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Xiaoyang Zhou
Third Military Medical University
11Publications
8H-index
349Citations
Publications 9
Newest
#1Dong Niu (ZJU: Zhejiang University)H-Index: 8
#2Hong-Jiang Wei (Yunnan Agricultural University)H-Index: 6
Last.Luhan YangH-Index: 16
view all 22 authors...
Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizont...
159 CitationsSource
#1Yong WangH-Index: 11
#2Yinan DuH-Index: 4
Last.Hong WeiH-Index: 21
view all 9 authors...
Donor major histocompatibility complex class I (MHC I) molecules are the main targets of the host immune response after organ allotransplantation. Whether and how MHC I-deficiency of pig donor tissues affects rejection after xenotransplantation has not been assessed. Beta2-microglobulin (B2M) is indispensable for the assembly of MHC I receptors and therefore provides an effective target to disrupt cell surface MHC I expression. Here, we report the one-step generation of mutant pigs with targeted...
8 CitationsSource
#1Xiaoyang Zhou (Third Military Medical University)H-Index: 8
#2Lulu Wang (Third Military Medical University)H-Index: 6
Last.Hong Wei (Third Military Medical University)H-Index: 21
view all 12 authors...
Precise genetic mutation of model animals is highly valuable for functional investigation of human mutations. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-induced homology-directed repair (HDR) is usually used for precise genetic mutation, being limited by the relatively low efficiency compared with that of non-homologous end joining (NHEJ). Although inhibition of NHEJ was shown to enhance HDR-derived mutation, in this work, without inhibition of ...
35 CitationsSource
#1Jin PengH-Index: 1
#2Yong WangH-Index: 11
Last.Pumin ZhangH-Index: 36
view all 14 authors...
Precise genome modification in large domesticated animals is desirable under many circumstances. In the past it is only possible through lengthy and burdensome cloning procedures. Here we attempted to achieve that goal through the use of the newest genome-modifying tool CRISPR/Cas9. We set out to knockin human albumin cDNA into pig Alb locus for the production of recombinant human serum albumin (rHSA). HSA is a widely used human blood product and is in high demand. We show that homologous recomb...
41 CitationsSource
#1Yong WangH-Index: 11
#2Yinan DuH-Index: 4
Last.Hong WeiH-Index: 21
view all 14 authors...
Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as 100%. Meanwhile, we carefully analyzed the Npc1l1 sgRNA:Cas9-mediated on- and off-target mutations in ...
64 CitationsSource
#1Yong Wang (Third Military Medical University)H-Index: 11
#2Xiaoyang Zhou (Third Military Medical University)H-Index: 8
Last.Hong Wei (Third Military Medical University)H-Index: 21
view all 8 authors...
The meganuclease I-SceI has been effectively used to facilitate transgenesis in fish eggs for nearly a decade. I-SceI-mediated transgenesis is simply via embryo cytoplasmic microinjection and only involves plasmid vectors containing I-SceI recognition sequences, therefore regarding the transgenesis process and application of resulted transgenic organisms, I-SceI-mediated transgenesis is of minimal bio-safety concerns. However, currently no transgenic mammals derived from I-SceI-mediated transgen...
11 CitationsSource
#1Wen Jiang (Third Military Medical University)H-Index: 2
#2Xiaoyang Zhou (Third Military Medical University)H-Index: 8
Last.Hong Wei (Third Military Medical University)H-Index: 21
view all 7 authors...
Xenogeneic skin, especially porcine skin, has already been used to cover large wounds in clinic practice of wound care. Our previous data showed that transgenic expression of human cytoxic T-lymphocyte associated antigen4-immunoglobulin (hCTLA4Ig) in murine skin graft remarkably prolonged its survival in xenogeneic burn wounds without extensive immunosuppression in recipients, suggesting that transgenic hCTLA4Ig expression in skin graft may be an effective and safe method to prolong its survival...
2 CitationsSource
#1Yong Wang (Third Military Medical University)H-Index: 11
#2Haihong Hu (ZJU: Zhejiang University)H-Index: 11
Last.Hong Wei (Third Military Medical University)H-Index: 21
view all 15 authors...
Cytochomosome P450 enzymes (CYP) are heme-containing monooxygenases responsible for oxidative metabolism of many exogenous and endogenous compounds including drugs. The species difference of CYP limits the extent to which data obtained from animals can be translated to humans in pharmacodynamics or pharmacokinetics studies. Transgenic expression of human CYP in animals lacking or with largely reduced endogenous CYP counterparts is recognized as an ideal strategy to correct CYP species difference...
11 CitationsSource
#1Yong Wang (Third Military Medical University)H-Index: 11
#2Yong-tao Song (Third Military Medical University)H-Index: 1
Last.Hong Wei (Third Military Medical University)H-Index: 21
view all 9 authors...
Lentiviral transgenesis is now recognized as an extremely efficient and cost-effective method to produce transgenic animals. Transgenes delivered by lentiviral vectors exhibited inheritable expression in many species including those which are refractory to genetic modification such as non-human primates. However, epigenetic modification was frequently observed in lentiviral integrants, and transgene expression found to be inversely correlated with methylation density. Recent data showed that abo...
17 CitationsSource
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