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Daesik Kim
Seoul National University
65Publications
12H-index
2,045Citations
Publications 65
Newest
#1Daesik KimH-Index: 12
#2Kevin Luk (UMMS: University of Massachusetts Medical School)H-Index: 2
Last.Jinsoo KimH-Index: 49
view all 4 authors...
Programmable nucleases and deaminases, which include zinc-finger nucleases, transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA-guided base editors, are now widely...
#1Daesik Kim (SNU: Seoul National University)H-Index: 12
#2Da-eun Kim (SNU: Seoul National University)H-Index: 3
Last.Jin-Soo Kim (SNU: Seoul National University)H-Index: 52
view all 5 authors...
Adenine base editors1 enable efficient targeted adenine-to-guanine single nucleotide conversions to induce or correct point mutations in human cells, animals, and plants1–4. Here we present a modified version of Digenome-seq, an in vitro method for identifying CRISPR (clustered regularly interspaced short palindromic repeats)-induced double-strand breaks using whole-genome sequencing5–8, to assess genome-wide target specificity of adenine base editors. To produce double-strand breaks at sites co...
#1Jaeyoung Yoon (SNU: Seoul National University)H-Index: 3
#2Yunsik Joo (SNU: Seoul National University)H-Index: 3
Last.Yongtaek Hong (SNU: Seoul National University)H-Index: 25
view all 9 authors...
#1Jaeyoung Yoon (SNU: Seoul National University)H-Index: 3
#2Yunsik Joo (SNU: Seoul National University)H-Index: 3
Last.Yongtaek Hong (SNU: Seoul National University)H-Index: 25
view all 9 authors...
#1Taehoon Kim (SNU: Seoul National University)H-Index: 34
#2Daesik Kim (SNU: Seoul National University)H-Index: 12
Last.Yongtaek Hong (SNU: Seoul National University)H-Index: 25
view all 6 authors...
Conductive thin films are typically subject to crack formation and propagation under tensile strain, turning into insulating films due to complete breakage at large strain. However, if such crack propagation can be intentionally designed, repetitive resistance change can be obtained and used for implementation of high-performance strain sensors that are suitable for biocompatible and stretchable electronic applications. In this work, therefore, we introduce a fiber-reinforced region, which is fo...
#1Jungjoon K. LeeH-Index: 2
#2Euihwan Jeong (SNU: Seoul National University)H-Index: 5
Last.Seokjoong KimH-Index: 14
view all 11 authors...
The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated Cas9 variants are often poorly efficient in human cells. Here, we develop a directed evolution approach in E. coli to obtain Sniper-Cas9, which shows high specificities without killing on-target activities in human cells. Unlike other engineered Cas...
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