Staffan Strömblad
Karolinska Institutet
CellMolecular biologyIntegrinBiologyCell biology
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Publications 75
#1Tânia D. F. Costa (KI: Karolinska Institutet)H-Index: 2
#2Staffan Strömblad (KI: Karolinska Institutet)H-Index: 30
ABSTRACTNormal cells exposed to cancer-causing events respond by triggering cellular senescence, a stress response which halts cell proliferation and constitutes a protective anti-cancer barrier. W...
#1Anindya Gupta (Uppsala University)H-Index: 3
#2Veronica Larsson (KI: Karolinska Institutet)
Last. Carolina Wählby (Uppsala University)H-Index: 22
view all 5 authors...
Cell migration is pivotal for their development, physiology and disease treatment. A single cell on a 2D surface can utilize continuous or discontinuous migration modes. To comprehend the cell migration, an adequate quantification for single cell-based analysis is crucial. An automatized approach could alleviate tedious manual analysis, facilitating large-scale drug screening. Supervised deep learning has shown promising outcomes in computerized microscopy image analysis. However, their implicat...
#1Nicolas PielawskiH-Index: 1
#2Jianjiang HuH-Index: 1
Last. Carolina WählbyH-Index: 22
view all 4 authors...
Traction Force Microscopy (TFM) is a technique used to determine the tensions that a biological cell conveys to the underlying surface. Typically, TFM requires culturing cells on gels with fluorescent beads, followed by bead displacement calculations. We present a new method allowing to predict those forces from a regular fluorescent image of the cell. Using Deep Learning, we trained a Bayesian Neural Network adapted for pixel regression of the forces and show that it generalises on different ce...
#1Alejandra Gonzalez-Beltran (University of Oxford)H-Index: 18
#2Paola MasuzzoH-Index: 8
Last. Lennart Martens (UGent: Ghent University)H-Index: 49
view all 25 authors...
Cell migration research has become a high-content field. However, the quantitative information encapsulated in these complex and high-dimensional datasets is not fully exploited due to the diversity of experimental protocols and non-standardised output formats. In addition, typically the datasets are not open for reuse. Making the data open and Findable, Accessible, Interoperable, and Reusable (FAIR) will enable meta-analysis, data integration, and data mining. Standardised data formats and cont...
#1Tânia D. F. Costa (KI: Karolinska Institutet)H-Index: 2
#2Ting Zhuang (KI: Karolinska Institutet)H-Index: 4
Last. Staffan Strömblad (KI: Karolinska Institutet)H-Index: 30
view all 22 authors...
Overcoming cellular growth restriction, including the evasion of cellular senescence, is a hallmark of cancer. We report that PAK4 is overexpressed in all human breast cancer subtypes and associated with poor patient outcome. In mice, MMTV-PAK4 overexpression promotes spontaneous mammary cancer, while PAK4 gene depletion delays MMTV-PyMT driven tumors. Importantly, PAK4 prevents senescence-like growth arrest in breast cancer cells in vitro, in vivo and ex vivo, but is not needed in non-immortali...
3 CitationsSource
#1John G. Lock (UNSW: University of New South Wales)H-Index: 18
#2Francesco Baschieri (French Institute of Health and Medical Research)H-Index: 5
Last. Martin J. Humphries (University of Manchester)H-Index: 79
view all 7 authors...
An understanding of the mechanisms whereby cell adhesion complexes (ACs) relay signals bidirectionally across the plasma membrane is necessary to interpret the role of adhesion in regulating migration, differentiation, and growth. A range of AC types has been defined, but to date all have similar compositions and are dependent on a connection to the actin cytoskeleton. Recently, a new class of AC has been reported that normally lacks association with both the cytoskeleton and integrin-associated...
3 CitationsSource
#1Parisa Rabieifar (KI: Karolinska Institutet)H-Index: 1
#2Ting Zhuang (KI: Karolinska Institutet)H-Index: 4
Last. Staffan Strömblad (KI: Karolinska Institutet)H-Index: 30
view all 5 authors...
p21-activated protein kinases (PAKs) are serine/threonine kinases functioning as downstream effectors of the small GTPases Rac1 and Cdc42. Members of the PAK family are overexpressed in human breast cancer, but their role in mammary gland development is not fully explored. Here we examined the functional role of PAK4 in mammary gland development by creating a mouse model of MMTV-Cre driven conditional PAK4 gene depletion in the mammary gland. The PAK4 conditional knock-out mice were born healthy...
1 CitationsSource
#1Philipp Jungebluth (KI: Karolinska Institutet)H-Index: 21
#2Johannes C. Haag (KI: Karolinska Institutet)H-Index: 11
Last. Paolo Macchiarini (KI: Karolinska Institutet)H-Index: 45
view all 15 authors...
#1John G. Lock (KI: Karolinska Institutet)H-Index: 18
#2Matthew Jones (Wellcome Trust Centre for Cell-Matrix Research)H-Index: 18
Last. Staffan Strömblad (KI: Karolinska Institutet)H-Index: 30
view all 10 authors...
Adhesion to the extracellular matrix persists during mitosis in most cell types. However, while classical adhesion complexes, such as focal adhesions, do and must disassemble to enable mitotic rounding, the mechanisms of residual mitotic cell–extracellular matrix adhesion remain undefined. Here, we identify ‘reticular adhesions’, a class of adhesion complex that is mediated by integrin αvβ5, formed during interphase, and preserved at cell–extracellular matrix attachment sites throughout cell div...
15 CitationsSource
#1Staffan Strömblad (KI: Karolinska Institutet)H-Index: 30
#2John G. Lock (UNSW: University of New South Wales)H-Index: 18
1 CitationsSource