Débora Campanella Bastos
State University of Campinas
PathologyMolecular biologyFatty acid synthaseCancer researchMedicine
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Publications 24
#1Andréia Ferreira Do Carmo (UFRN: Federal University of Rio Grande do Norte)H-Index: 1
#2Mauricio Rocha Dourado (State University of Campinas)H-Index: 7
Last. Renato Corrêa Viana Casarin (State University of Campinas)H-Index: 21
view all 15 authors...
Abstract Stanniocalcin 2 (STC2), a glycoprotein that regulates calcium and phosphate homeostasis during mineral metabolism, appears to display multiple roles in tumorigenesis and cancer progression. This study aimed to access the prognostic value of STC2 in oral squamous cell carcinoma (OSCC) and its implications in oral tumorigenesis. STC2 expression was examined in 2 independent cohorts of OSCC tissues by immunohistochemistry. A loss-of-function strategy using shRNA targeting STC2 was employed...
#1Iara Gonçalves de Aquino (State University of Campinas)
#1Iara Gonçalves De Aquino (State University of Campinas)
Last. Edgard Graner (State University of Campinas)H-Index: 36
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Abstract Objective Fatty acid synthase (FASN) is overexpressed in several human cancers, including oral squamous cell carcinoma (OSCC). TVB-3166 is a recently described FASN inhibitor with antitumor effects and potential clinical relevance. The objective of this study was to evaluate the effects of TVB-3166 on OSCC cell lines. Materials and methods The OSCC cell line SCC-9 modified to express ZsGreen (ZsG) (SCC-9 ZsG) and its in vivo selected metastatic derivative LN-1A were used to evaluate ant...
#1João Figueira Scarini (State University of Campinas)
#2Larissa Fernandes Rosa (State University of Campinas)
Last. Roman CarlosH-Index: 16
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OBJECTIVE: To analyze the gene and immunohistochemical expression of HIF-1alpha, GLUT-1, FASN and Adipophilin in normal salivary gland (NSG), pleomorphic adenoma (PA) and carcinoma ex-pleomorphic adenoma (CXPA) samples. MATERIAL AND METHODS: The gene expression was investigated by the Real Time PCR (qRT-PCR) method in 9 samples of frozen tissues of normal salivary gland, 13 PA and 10 CXPA. We validated the reactions by immunohistochemistry on 20 samples from NSG, 85 PA and 44 CXPA. RESULTS: Our ...
#1Luciana Yamamoto Almeida (State University of Campinas)H-Index: 5
#2Flávia Sammartino Mariano (State University of Campinas)H-Index: 8
Last. Edgard Graner (State University of Campinas)H-Index: 36
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Purpose Fatty acid synthase (FASN), the multifunctional enzyme responsible for endogenous fatty acid synthesis, is highly expressed and associated with poor prognosis in several human cancers, including melanoma. Our group has previously shown that pharmacological inhibition of FASN with orlistat decreases proliferation, promotes apoptosis, and reduces the metastatic spread of B16-F10 cells in experimental models of melanoma. While most of the orlistat antitumor properties seem to be closely rel...
1 CitationsSource
Introduction Over-expression of fatty acid synthase (fasn) is associated with poor prognosis in several human cancers, including oral squamous cell carcinoma (OSCC). Objective To evaluate the effects of the fasn inhibitor TVB-3166 on the cell viability, morphology, cell cycle and migration of SCC-9 and SCC-9 LN-1 cells derived from tongue OSCC. Study Design Cell viability was determined using standard MTT assay after 48 hours of treatment with increasing doses of TVB-3166. IC50 was calculated wi...
Carcinoma ex-pleomorphic adenoma (CXPA) arises in pleomorphic adenoma (PA) and with an increase in lipogenesis during its malignant transformation. The increase in lipogenesis is a feature of the cancer cells and it is characterized by the increase of fatty acid (FA) synthesis. This way, the overexpression of the enzyme fatty acid synthase (fasn) has been correlated. In the same way, the increase of glycolytic metabolism in neoplastic cells is due to the high expression of glycolytic enzymes and...
Cancer stem cells (CSCs) comprise subpopulation of tumor cells associated with cancer initiation, progression, and treatment failures found in several malignancies, including oral squamous cells carcinoma (OSCC). Currently, among the methods employed for detection and isolation of CSCs the most used is flow cytometry immunophenotyping. The expression of surface markers for CSCs in SCC-9 cells, derived from tongue OSCC, has not been described in the literature. Objective: To identify and compare ...
The aim of this study was to compare the effect of pharmacologic inhibition of 3 different catalytic domains of fatty acid synthase (FASN) on the cell cycle and apoptosis and associate with the morphologic alterations in squamous cell carcinoma 9 (SCC-9) cells. SCC-9 cells were treated with C75, triclosan (TCS), and orlistat (ORL) (inhibitors of KS, ER, and TE domains of FASN) for 24 to 48 hours and analyzed by flow cytometry and phase contrast microscopy. FASN inhibition with C75, ORL means Orl...
Last. Massimo LodaH-Index: 103
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#1Mauricio Rocha Dourado (State University of Campinas)H-Index: 7
#2Johanna Korvala (University of Oulu)H-Index: 9
Last. Tuula Salo (OUH: Oulu University Hospital)H-Index: 74
view all 12 authors...
ABSTRACTAs one of the most abundant constituents of the tumour microenvironment (TME), cancer-associated fibroblasts (CAF) display critical roles during tumour progression and metastasis. Multiple ...
7 CitationsSource