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Tatsuya Takemoto
University of Tokushima
EnhancerNeural plateSOX2GeneticsBiology
47Publications
14H-index
1,300Citations
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Publications 46
Newest
#1Taichi Nakatani (Kobe University)
#2Mitsuhiro Iwasaki (Kobe University)H-Index: 1
Last. Hideki Enomoto (Kobe University)H-Index: 30
view all 10 authors...
Missense mutations of the RET gene have been identified in both multiple endocrine neoplasia (MEN) type 2A/B and Hirschsprung disease (HSCR: congenital absence of the enteric nervous system, ENS). Current consensus holds that MEN2A/B and HSCR are respectively caused by activating and inactivating RET mutations. However, the biological significance of RET missense mutations in vivo has not been fully elucidated. In the present study, we introduced one MEN2B-associated (M918T) and two HSCR-associa...
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#1Machiko Teramoto (Kyoto Sangyo University)H-Index: 1
#2Ryo Sugawara (Kyoto Sangyo University)H-Index: 1
Last. Hisato Kondoh (Kyoto Sangyo University)H-Index: 55
view all 8 authors...
In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. Previous studies using hypomorphic Sox2 alleles have indicated that reduced SOX2 expression causes the esophageal epithelium to display some respiratory organ characteristics. In the present study, we produced mouse embryo...
2 CitationsSource
#1Hideaki Iida (Kyoto Sangyo University)H-Index: 2
#2Yoko Furukawa (Kyoto Sangyo University)
Last. Hisato Kondoh (Kyoto Sangyo University)H-Index: 55
view all 7 authors...
The transcription factor (TF) SOX2 regulates various stem cells and tissue progenitors via functional interactions with cell type-specific partner TFs that co-bind to enhancer sequences. Neural progenitors are the major embryonic tissues where SOX2 assumes central regulatory roles. In order to characterize the partner TFs of SOX2 in neural progenitors, we investigated the regulation of the D1 enhancer of the Sox2 gene, which is activated in the embryonic neural tube (NT) and neural crest (NC), u...
Source
#1Tatsuya Takemoto (University of Tokushima)H-Index: 14
: The CRISPR/Cas9 system is a powerful tool for generation of genetically modified mice. In conventional protocols, Cas9 protein (or mRNA) and sgRNA are introduced into zygotes by microinjection. However, microinjection requires special skill and is too time-consuming to treat zygotes on a large scale. Recently, we have developed a simple electroporation method which generates genetically modified mice with high efficiency. Here, we describe our method GEEP (genome editing by electroporation of ...
Source
#1Machiko Teramoto (Kyoto Sangyo University)H-Index: 1
#2Ryo Sugawara (Kyoto Sangyo University)H-Index: 1
Last. Hisato Kondoh (Kyoto Sangyo University)H-Index: 55
view all 8 authors...
In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. Previous studies using hypomorphic Sox2 alleles have indicated that reduced SOX2 expression causes the esophageal epithelium to display some respiratory organ characteristics. In the present study, we produced mouse embryo...
Source
#1Daisuke Sugiura (University of Tokushima)H-Index: 7
#2Takumi Maruhashi (University of Tokushima)H-Index: 8
Last. Taku Okazaki (University of Tokushima)H-Index: 41
view all 7 authors...
Targeted blockade of PD-1 using checkpoint inhibitor immunotherapies can activate T cells to destroy tumors. PD-1 is believed to function mainly at the effector but not in the activation phase of T cell responses, yet how PD-1 function is restricted at the activation stage is currently unknown. Here we demonstrate that CD80 interacts with PD-L1 in cis on antigen presenting cells (APCs) to disrupt PD-L1/PD-1 binding. Subsequently, PD-L1 cannot engage PD-1 to inhibit T cell activation when APCs ex...
50 CitationsSource
#1Shiori Yamamoto (Nagoya University)H-Index: 2
#2Yuji Uchida (Nagoya University)H-Index: 1
Last. Atsushi Kuroiwa (Nagoya University)H-Index: 36
view all 12 authors...
: To elucidate the role of Hox genes in limb cartilage development, we identified the target genes of HOXA11 and HOXA13 by ChIP-Seq. The ChIP DNA fragment contained evolutionarily conserved sequences and multiple highly conserved HOX binding sites. A substantial portion of the HOXA11 ChIP fragment overlapped with the HOXA13 ChIP fragment indicating that both factors share common targets. Deletion of the target regions neighboring Bmp2 or Tshz2 reduced their expression in the autopod suggesting t...
2 CitationsSource
#1Fuminori Tanihara (University of Tokushima)H-Index: 10
#2Maki Hirata (University of Tokushima)H-Index: 5
Last. Takeshige Otoi (University of Tokushima)H-Index: 31
view all 9 authors...
: Recently, we established the GEEP ("gene editing by electroporation of Cas9 protein") method, in which the CRISPR/Cas9 system, consisting of a Cas9 protein and single guide RNA (sgRNA), is introduced into pig zygotes by electroporation and thus induces highly efficient targeted gene disruption. In this study, we examined the effects of sgRNA on the blastocyst formation of porcine embryos and evaluated their genome-editing efficiency. To produce an animal model for diabetes, we targeted PDX-1 (...
6 CitationsSource
#1Fuminori Tanihara (University of Tokushima)H-Index: 10
#2Maki Hirata (University of Tokushima)H-Index: 5
Last. Takeshige Otoi (University of Tokushima)H-Index: 31
view all 9 authors...
TP53 (which encodes p53) is one of the most frequently mutated genes in cancers. In this study, we generated TP53-mutant pigs by gene editing via electroporation of the Cas9 protein (GEEP), a process that involves introducing the Cas9 protein and single-guide RNA (sgRNA) targeting exon 3 and intron 4 of TP53 into in vitro-fertilized zygotes. Zygotes modified by the sgRNAs were transferred to recipients, two of which gave birth to a total of 11 piglets. Of those 11 piglets, 9 survived. Molecular ...
14 CitationsSource
#1Tetsuro KomatsuH-Index: 2
#2Akihito HaradaH-Index: 15
Last. Yasuyuki OhkawaH-Index: 30
view all 12 authors...
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