Tatsuya Takemoto
University of Tokushima
Publications 43
#1Tatsuya Takemoto (University of Tokushima)H-Index: 13
#1Machiko Teramoto (Kyoto Sangyo University)
#2Ryo Sugawara (Kyoto Sangyo University)
Last.Hisato Kondoh (Kyoto Sangyo University)H-Index: 58
view all 8 authors...
In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. Previous studies using hypomorphic Sox2 alleles have indicated that reduced SOX2 expression causes the esophageal epithelium to display some respiratory organ characteristics. In the present study, we produced mouse embryo...
#1Daisuke Sugiura (University of Tokushima)H-Index: 8
#2Takumi Maruhashi (University of Tokushima)H-Index: 7
Last.Taku Okazaki (University of Tokushima)H-Index: 40
view all 7 authors...
Targeted blockade of PD-1 using checkpoint inhibitor immunotherapies can activate T cells to destroy tumors. PD-1 is believed to function mainly at the effector but not in the activation phase of T cell responses, yet how PD-1 function is restricted at the activation stage is currently unknown. Here we demonstrate that CD80 interacts with PD-L1 in cis on antigen presenting cells (APCs) to disrupt PD-L1/PD-1 binding. Subsequently, PD-L1 cannot engage PD-1 to inhibit T cell activation when APCs ex...
15 CitationsSource
#1Shiori Yamamoto (Nagoya University)H-Index: 2
#2Yuji Uchida (Nagoya University)H-Index: 1
Last.Atsushi Kuroiwa (Nagoya University)H-Index: 33
view all 12 authors...
1 CitationsSource
#1Fuminori Tanihara (University of Tokushima)H-Index: 9
#2Maki Hirata (University of Tokushima)H-Index: 3
Last.Takeshige Otoi (University of Tokushima)H-Index: 30
view all 9 authors...
3 CitationsSource
#1Akihito Harada (Kyushu University)H-Index: 14
#2Kazumitsu Maehara (Kyushu University)H-Index: 15
Last.Yasuyuki Ohkawa (Kyushu University)H-Index: 29
view all 17 authors...
Regulation of gene expression requires selective incorporation of histone H3 variant H3.3 into chromatin. Histone H3.3 has several subsidiary variants but their functions are unclear. Here we characterize the function of histone H3.3 sub-variant, H3mm7, which is expressed in skeletal muscle satellite cells. H3mm7 knockout mice demonstrate an essential role of H3mm7 in skeletal muscle regeneration. Chromatin analysis reveals that H3mm7 facilitates transcription by forming an open chromatin struct...
#1Fuminori Tanihara (University of Tokushima)H-Index: 9
#2Maki Hirata (University of Tokushima)H-Index: 3
Last.Takeshige Otoi (University of Tokushima)H-Index: 30
view all 9 authors...
TP53 (which encodes p53) is one of the most frequently mutated genes in cancers. In this study, we generated TP53-mutant pigs by gene editing via electroporation of the Cas9 protein (GEEP), a process that involves introducing the Cas9 protein and single-guide RNA (sgRNA) targeting exon 3 and intron 4 of TP53 into in vitro-fertilized zygotes. Zygotes modified by the sgRNAs were transferred to recipients, two of which gave birth to a total of 11 piglets. Of those 11 piglets, 9 survived. Molecular ...
5 CitationsSource
#1Kazumasa Nishio (University of Tokushima)H-Index: 2
#2Fuminori Tanihara (University of Tokushima)H-Index: 9
Last.Takeshige Otoi (University of Tokushima)H-Index: 30
view all 8 authors...
Contents This study was conducted to determine suitable conditions for an experimental method in which the CRISPR/Cas9 system is introduced into in vitro-produced porcine zygotes by electroporation. In the first experiment, when putative zygotes derived from in vitro fertilization (IVF) were electroporated by either unipolar or bipolar pulses, keeping the voltage, pulse duration and pulse number fixed at 30 V/mm, 1 msec and five repeats, respectively, the rate of blastocyst formation from zygote...
4 CitationsSource
#1Sayumi FujimoriH-Index: 1
#2Izumi OhigashiH-Index: 19
Last.Shinji TakadaH-Index: 45
view all 5 authors...