Masaru Miyagi
Case Western Reserve University
Publications 125
#1Maria de la Fuente (Case Western Reserve University)H-Index: 7
#2Xu Han (Case Western Reserve University)H-Index: 1
Last.Marvin T. Nieman (Case Western Reserve University)H-Index: 16
view all 4 authors...
Protease-activated receptors (PARs) are G-protein-coupled receptors that are activated by proteolysis of the N-terminus, which exposes a tethered ligand that interacts with the receptor. Numerous studies have focused on the signaling pathways mediated by PARs. However, the structural basis for initiation of these pathways is unknown. Here, we describe a strategy for the expression and purification of PAR4. This is the first PAR family member to be isolated without stabilizing modifications for b...
#1I-Ju Yeh (Case Western Reserve University)H-Index: 1
#2Emily L. Esakov (Cleveland Clinic Lerner Research Institute)
Last.Monica M. Montano (Case Western Reserve University)H-Index: 16
view all 6 authors...
Epigenetic modifications are known to play critical roles in the expression of genes related to differentiation and dedifferentiation. Histone lysine demethylase KDM5B (PLU-1) catalyzes the demethylation of histone H3 on Lys 4 (H3K4), which results in the repression of gene expression. KDM5B is involved in regulation of luminal and basal cell specific gene expression in breast cancers. However, the mechanisms by which KDM5B is regulated in breast cancer, in particular in response to post-transla...
#1Neetu M. Gulati (Case Western Reserve University)H-Index: 6
#2Masaru Miyagi (Case Western Reserve University)H-Index: 34
Last.Phoebe L. Stewart (Case Western Reserve University)H-Index: 43
view all 5 authors...
Background: Human papillomavirus (HPV) is linked to nearly all cases of cervical cancer. Despite available vaccines, a deeper understanding of the immune response to HPV is needed. Human α-defensin 5 (HD5), an innate immune effector peptide, blocks infection of multiple serotypes of HPV, including high-risk HPV16. While a common mechanism of α-defensin anti-viral activity against nonenveloped viruses such as HPV has emerged, there is limited understanding of how α-defensins bind to viral capsids...
#1Christopher Hager (Case Western Reserve University)H-Index: 4
#2N. Isham (Case Western Reserve University)H-Index: 11
Last.Mahmoud A. Ghannoum (Case Western Reserve University)H-Index: 57
view all 7 authors...
ABSTRACT Dysbiosis of the gut microbiome has been implicated in inflammatory bowel diseases. We have shown that levels of Candida tropicalis, along with those of Escherichia coli and Serratia marcescens, are significantly elevated in Crohn’s disease (CD) patients. Here, we evaluated the ability of a novel probiotic to prevent and treat polymicrobial biofilms (PMB) formed by C. tropicalis with E. coli and S. marcescens. Since Candida albicans has been reported to be elevated in CD patients, we in...
2 CitationsSource
#1Antja Voy Hartley (IU: Indiana University)H-Index: 4
#2Benlian Wang (Case Western Reserve University)H-Index: 18
Last.Tao Lu (IU: Indiana University)H-Index: 18
view all 6 authors...
Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in the US with a staggeringly less than 14% survival rate for patients with metastatic disease. This poor clinical outcome for CRC patients is largely attributed to the lack of understanding of the factors that drive CRC progression, leading to a dearth of available treatment strategies for this disease. Today, treatment modalities and clinical management of colorectal cancer (CRC) are fundamentally based on the success...
#1Asifa K. Zaidi (Case Western Reserve University)H-Index: 1
#2Krishna Vukoti (Case Western Reserve University)H-Index: 3
Last.Masaru MiyagiH-Index: 34
view all 5 authors...
Dauer larva is an alternative developmental stage of Caenorhabditis elegans ( C. elegans ) that occurs when the environmental condition is unfavorable for growth. Little is known regarding how the proteome of dauer larvae respond to poor environmental growth conditions. Such knowledge is expected to help understand the survival mechanism(s) of dauer larvae. In order to uncover the proteome differences between dauer larvae and normally developed third stage larvae (L3), an L2 stage larvae was sta...
#1Yiqing Zhao (Case Western Reserve University)H-Index: 5
#2Shuliang Zhao (Case Western Reserve University)
Last.Zhenghe Wang (Case Western Reserve University)H-Index: 20
view all 14 authors...
Protein tyrosine nitration occurs under both physiological and pathological conditions. However, enzymes that remove this protein modification have not yet been identified. Here we report that the pseudo-phosphatase domain of protein tyrosine receptor T (PTPRT) is a denitrase that removes nitro-groups from tyrosine residues in paxillin. PTPRT normally functions as a tumor suppressor and is frequently mutated in a variety of human cancers including colorectal cancer. We demonstrate that some of t...
#1Kazunori D. Yamada (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 10
#2Satoshi Omori (Tohoku University)H-Index: 2
Last.Masaru Miyagi (Case Western Reserve University)H-Index: 34
view all 4 authors...
Background N-terminal acetylation is one of the most common protein modifications in eukaryotes and occurs co-translationally when the N-terminus of the nascent polypeptide is still attached to the ribosome. This modification has been shown to be involved in a wide range of biological phenomena such as protein half-life regulation, protein-protein and protein-membrane interactions, and protein subcellular localization. Thus, accurately predicting which proteins receive an acetyl group based on t...
2 CitationsSource
#1Jessica M. Berthiaume (Case Western Reserve University)H-Index: 9
#2Chia heng Hsiung (CMU: Central Michigan University)H-Index: 1
Last.Mariana G. Rosca (CMU: Central Michigan University)H-Index: 17
view all 8 authors...
Diabetic cardiomyopathy is preceded by mitochondrial alterations, and progresses to heart failure. We studied whether treatment with methylene blue (MB), a compound that was reported to serve as an alternate electron carrier within the mitochondrial electron transport chain (ETC), improves mitochondrial metabolism and cardiac function in type 1 diabetes. MB was administered at 10 mg/kg/day to control and diabetic rats. Both echocardiography and hemodynamic studies were performed to assess cardia...
8 CitationsSource
#1Sireesha Mamillapalli (WSU: Wichita State University)H-Index: 1
#2Masaru Miyagi (Case Western Reserve University)H-Index: 34
Last.James G. Bann (WSU: Wichita State University)H-Index: 13
view all 3 authors...
The major immunogenic component of the current anthrax vaccine, anthrax vaccine adsorbed (AVA) is protective antigen (PA). We have shown recently that the thermodynamic stability of PA can be significantly improved by binding to the Von-Willebrand factor A (VWA) domain of capillary morphogenesis protein 2 (CMG2), and improvements in thermodynamic stability may improve storage and long-term stability of PA for use as a vaccine. In order to understand the origin of this increase in stability, we h...