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Georg Büldt
Moscow Institute of Physics and Technology
117Publications
31H-index
3,430Citations
Publications 117
Newest
#1Kirill KovalevH-Index: 5
#2Vitaly Polovinkin (CNRS: Centre national de la recherche scientifique)H-Index: 7
Last.Dmitry Bratanov (CNRS: Centre national de la recherche scientifique)H-Index: 1
view all 15 authors...
Rhodopsins are the most universal biological light-energy transducers and abundant phototrophic mechanisms that evolved on Earth and have a remarkable diversity and potential for biotechnological applications. Recently, the first sodium-pumping rhodopsin KR2 from Krokinobacter eikastus was discovered and characterized. However, the existing structures of KR2 are contradictory, and the mechanism of Na + pumping is not yet understood. Here, we present a structure of the cationic (non H + ) light-d...
#1Ivan Maslov (MIPT: Moscow Institute of Physics and Technology)H-Index: 1
#2Andrey O. Bogorodskiy (MIPT: Moscow Institute of Physics and Technology)H-Index: 2
Last.Valentin I. Gordeliy (MIPT: Moscow Institute of Physics and Technology)H-Index: 21
view all 12 authors...
Research on halophilic microorganisms is important due to their relation to fundamental questions of survival of living organisms in a hostile environment. Here we introduce a novel method to stain halophiles with MitoTracker fluorescent dyes in their growth medium. The method is based on membrane-potential sensitive dyes, which were originally used to label mitochondria in eukaryotic cells. We demonstrate that these fluorescent dyes provide high staining efficiency and are beneficial for multi-...
#1D. Zabelskii (MIPT: Moscow Institute of Physics and Technology)H-Index: 1
#2A. V. Vlasov (MIPT: Moscow Institute of Physics and Technology)H-Index: 1
Last.A. V. Rogachev (JINR: Joint Institute for Nuclear Research)H-Index: 6
view all 15 authors...
#1M. A. Shevchenko (RAS: Russian Academy of Sciences)H-Index: 3
#2Andrey O. Bogorodskiy (MIPT: Moscow Institute of Physics and Technology)H-Index: 2
Last.Valentin Borshchevskiy (MIPT: Moscow Institute of Physics and Technology)H-Index: 11
view all 11 authors...
Susceptibility to fungal infection is commonly associated with impaired neutrophil responses. To study the mechanisms underlying this association, we investigated neutrophil recruitment to the conducting airway wall after Aspergillus fumigatus conidium inhalation in mouse models of drug-induced immunosuppression and antibody-mediated neutrophil depletion (neutropenia) by performing three-dimensional confocal laser-scanning microscopy of whole-mount primary bronchus specimens. Actin staining enab...
#1Oleksandr VolkovH-Index: 2
#2Kirill KovalevH-Index: 5
Last.Dieter Willbold (HHU: University of Düsseldorf)H-Index: 36
view all 13 authors...
INTRODUCTION Ion channels are integral membrane proteins that upon stimulation modulate the flow of ions across the cell or organelle membrane. The resulting electrical signals are involved in biological functions such as electrochemical transmission and information processing in neurons. Channelrhodopsins (ChRs) appear to be unusual channels. They belong to the large family of microbial rhodopsins, seven-helical transmembrane proteins containing retinal as chromophore. Photon absorption initiat...
#1Ivan Yu. Gushchin (MIPT: Moscow Institute of Physics and Technology)H-Index: 10
#2Igor Melnikov (European Synchrotron Radiation Facility)H-Index: 4
Last.Taras BalandinH-Index: 11
view all 16 authors...
One of the major and essential classes of transmembrane (TM) receptors, present in all domains of life, is sensor histidine kinases (HKs), parts of two-component signaling systems (TCS). The structural mechanisms of transmembrane signaling by these sensors are poorly understood. We present here crystal structures of the periplasmic sensor domain, the TM domain and the cytoplasmic HAMP domain of the Escherichia coli nitrate/nitrite sensor HK NarQ in the ligand-bound and mutated ligand-free states...
#1Florian Wruck (Trinity College, Dublin)H-Index: 2
#2Alexandros Katranidis (Forschungszentrum Jülich)H-Index: 6
Last.Martin Hegner (Trinity College, Dublin)H-Index: 35
view all 5 authors...
Protein biosynthesis is inherently coupled to cotranslational protein folding. Folding of the nascent chain already occurs during synthesis and is mediated by spatial constraints imposed by the ribosomal exit tunnel as well as self-interactions. The polypeptide’s vectorial emergence from the ribosomal tunnel establishes the possible folding pathways leading to its native tertiary structure. How cotranslational protein folding and the rate of synthesis are linked to a protein’s amino acid sequenc...
#1Andrii IshchenkoH-Index: 16
#2Ekaterina RoundH-Index: 10
Last.Taras BalandinH-Index: 11
view all 13 authors...
The complex of two membrane proteins, sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII), mediates negative phototaxis in halobacteria N. pharaonis. Upon light activation NpSRII triggers a signal transduction chain homologous to the two-component system in eubacterial chemotaxis. Here we report on crystal structures of the ground and active M-state of the complex in the space group I2 1 2 1 2 1. We demonstrate that the relative orientation of symmetrical parts of the dimer is pa...
#1M. NikolaevH-Index: 1
#2Ekaterina RoundH-Index: 10
Last.A. RoundH-Index: 1
view all 14 authors...
Membrane-like nanodiscs (ND) have become an important tool for the cell-free expression, solubilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs embedded in NDs would be of high importance for structural biology. However, despite considerable efforts we have been as yet unable to obtain crystals suitable for X-ray crystallography. In the present work, we show that an ND-trapped MP can be transferred into the cubic phase an...
#1Axel Baumann (Forschungszentrum Jülich)H-Index: 1
#2Silke Kerruth (FU: Free University of Berlin)H-Index: 4
Last.Kenichi Ataka (FU: Free University of Berlin)H-Index: 26
view all 7 authors...
Proper insertion, folding and assembly of functional proteins in biological membranes are key processes to warrant activity of a living cell. Here, we present a novel approach to trace folding and insertion of a nascent membrane protein leaving the ribosome and penetrating the bilayer. Surface Enhanced IR Absorption Spectroscopy selectively monitored insertion and folding of membrane proteins during cell-free expression in a label-free and non-invasive manner. Protein synthesis was performed in ...
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