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Georg Büldt
Moscow Institute of Physics and Technology
ChemistryBacteriorhodopsinRhodopsinBiochemistryCrystallography
96Publications
30H-index
2,866Citations
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Publications 99
Newest
#1K. KovalevH-Index: 5
#2Roman Astashkin (MIPT: Moscow Institute of Physics and Technology)H-Index: 2
Last. Antoine Royant (CNRS: Centre national de la recherche scientifique)H-Index: 18
view all 24 authors...
The light-driven sodium-pumping rhodopsin KR2 from Krokinobacter eikastus is the only non-proton cation active transporter with demonstrated potential for optogenetics. However, the existing structural data on KR2 correspond exclusively to its ground state, and show no sodium inside the protein, which hampers the understanding of sodium-pumping mechanism. Here we present crystal structure of the O-intermediate of the physiologically relevant pentameric form of KR2 at the resolution of 2.1 A, rev...
Source
#1Xiaoming Fan (CAS: Chinese Academy of Sciences)
#2Johnny Hendriks (Forschungszentrum Jülich)H-Index: 2
Last. Thomas Gensch (Forschungszentrum Jülich)H-Index: 28
view all 6 authors...
Single molecule localization microscopy (SMLM) allows the imaging of cellular structures with resolutions five to ten times below the diffraction limit of optical microscopy. It was originally introduced as a two-dimensional technique based on the localization of single emitters as projection onto the x-y imaging plane. The determination of the axial position of a fluorescent emitter is only possible by additional information. Here we report a method (spatial filter SMLM (SFSMLM)) that allows to...
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#1K. KovalevH-Index: 5
#2Dimitij Volkov (Forschungszentrum Jülich)H-Index: 1
Last. A. V. Rogachev (JINR: Joint Institute for Nuclear Research)H-Index: 7
view all 18 authors...
Rhodopsins are the most abundant light-harvesting proteins. A new family of rhodopsins, heliorhodopsins (HeRs), has recently been discovered. Unlike in the known rhodopsins, in HeRs the N termini face the cytoplasm. The function of HeRs remains unknown. We present the structures of the bacterial HeR-48C12 in two states at the resolution of 1.5 A, which highlight its remarkable difference from all known rhodopsins. The interior of HeR’s extracellular part is completely hydrophobic, while the cyto...
1 CitationsSource
#1A. V. Vlasov (MIPT: Moscow Institute of Physics and Technology)H-Index: 2
#2K. KovalevH-Index: 5
Last. Valentin I. Gordeliy (MIPT: Moscow Institute of Physics and Technology)H-Index: 23
view all 16 authors...
Membrane integral ATP synthases produce adenosine triphosphate, the universal “energy currency” of most organisms. However, important details of proton driven energy conversion are still unknown. We present the first high-resolution structure (2.3 A) of the in meso crystallized c-ring of 14 subunits from spinach chloroplasts. The structure reveals molecular mechanisms of intersubunit contacts in the c14-ring, and it shows additional electron densities inside the c-ring which form circles paralle...
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#1Dmitry Bratanov (Forschungszentrum Jülich)H-Index: 2
#2K. KovalevH-Index: 5
Last. Valentin I. GordeliyH-Index: 23
view all 29 authors...
Recently, two groups of rhodopsin genes were identified in large double-stranded DNA viruses. The structure and function of viral rhodopsins are unknown. We present functional characterization and high-resolution structure of an Organic Lake Phycodnavirus rhodopsin II (OLPVRII) of group 2. It forms a pentamer, with a symmetrical, bottle-like central channel with the narrow vestibule in the cytoplasmic part covered by a ring of 5 arginines, whereas 5 phenylalanines form a hydrophobic barrier in i...
7 CitationsSource
#1K. KovalevH-Index: 5
#2Vitaly Polovinkin (CNRS: Centre national de la recherche scientifique)H-Index: 1
Last. Valentin I. Gordeliy (MIPT: Moscow Institute of Physics and Technology)H-Index: 23
view all 15 authors...
Rhodopsins are the most universal biological light-energy transducers and abundant phototrophic mechanisms that evolved on Earth and have a remarkable diversity and potential for biotechnological applications. Recently, the first sodium-pumping rhodopsin KR2 from Krokinobacter eikastus was discovered and characterized. However, the existing structures of KR2 are contradictory, and the mechanism of Na + pumping is not yet understood. Here, we present a structure of the cationic (non H + ) light-d...
4 CitationsSource
#1D. Zabelskii (MIPT: Moscow Institute of Physics and Technology)H-Index: 1
#2A. V. Vlasov (MIPT: Moscow Institute of Physics and Technology)H-Index: 2
Last. Alexander I. Kuklin (JINR: Joint Institute for Nuclear Research)H-Index: 10
view all 15 authors...
2 CitationsSource
#1Ivan Maslov (MIPT: Moscow Institute of Physics and Technology)H-Index: 1
#2Andrey Bogorodskiy (MIPT: Moscow Institute of Physics and Technology)H-Index: 2
Last. Valentin Borshchevskiy (MIPT: Moscow Institute of Physics and Technology)H-Index: 11
view all 12 authors...
Research on halophilic microorganisms is important due to their relation to fundamental questions of survival of living organisms in a hostile environment. Here we introduce a novel method to stain halophiles with MitoTracker fluorescent dyes in their growth medium. The method is based on membrane-potential sensitive dyes, which were originally used to label mitochondria in eukaryotic cells. We demonstrate that these fluorescent dyes provide high staining efficiency and are beneficial for multi-...
2 CitationsSource
#1Marina Shevchenko (RAS: Russian Academy of Sciences)H-Index: 4
#2Andrey Bogorodskiy (MIPT: Moscow Institute of Physics and Technology)H-Index: 2
Last. Alexander M. Sapozhnikov (RAS: Russian Academy of Sciences)H-Index: 9
view all 11 authors...
Susceptibility to fungal infection is commonly associated with impaired neutrophil responses. To study the mechanisms underlying this association, we investigated neutrophil recruitment to the conducting airway wall after Aspergillus fumigatus conidium inhalation in mouse models of drug-induced immunosuppression and antibody-mediated neutrophil depletion (neutropenia) by performing three-dimensional confocal laser-scanning microscopy of whole-mount primary bronchus specimens. Actin staining enab...
1 CitationsSource
#1O. VolkovH-Index: 2
#2K. KovalevH-Index: 5
Last. Valentin I. Gordeliy (MIPT: Moscow Institute of Physics and Technology)H-Index: 23
view all 13 authors...
INTRODUCTION Ion channels are integral membrane proteins that upon stimulation modulate the flow of ions across the cell or organelle membrane. The resulting electrical signals are involved in biological functions such as electrochemical transmission and information processing in neurons. Channelrhodopsins (ChRs) appear to be unusual channels. They belong to the large family of microbial rhodopsins, seven-helical transmembrane proteins containing retinal as chromophore. Photon absorption initiat...
36 CitationsSource
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