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Joseph D. Ng
University of Alabama in Huntsville
89Publications
13H-index
806Citations
Publications 87
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#1Jiri BryndaH-Index: 15
Last.Pavlina RezacovaH-Index: 14
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Last.Ivana Kuta SmatanovaH-Index: 11
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#1Joseph D. NgH-Index: 13
#2James K. BairdH-Index: 15
Last.Noriko InoguchiH-Index: 1
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#1Noriko InoguchiH-Index: 1
#2Leighton CoatesH-Index: 19
Last.Joseph D. NgH-Index: 13
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1 CitationsSource
#1Noriko InoguchiH-Index: 1
#2Leighton CoatesH-Index: 19
Last.Joseph D. NgH-Index: 13
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Last.Joseph D. NgH-Index: 13
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Fluorescence can be a powerful tool to aid in the crystallization of proteins. In the trace-labeling approach, the protein is covalently derivatized with a high-quantum-yield visible-wavelength fluorescent probe. The final probe concentration typically labels ≤0.20% of the protein molecules, which has been shown to not affect the crystal nucleation or diffraction quality. The labeled protein is then used in a plate-screening experiment in the usual manner. As the most densely packed state of the...
19 CitationsSource
#1Joseph D. Ng (UAH: University of Alabama in Huntsville)H-Index: 13
#2James K. Baird (UAH: University of Alabama in Huntsville)H-Index: 15
Last.Sijay Huang (UAH: University of Alabama in Huntsville)H-Index: 2
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Neutron macromolecular crystallography (NMC) is the prevailing method for the accurate determination of the positions of H atoms in macromolecules. As neutron sources are becoming more available to general users, finding means to optimize the growth of protein crystals to sizes suitable for NMC is extremely important. Historically, much has been learned about growing crystals for X-ray diffraction. However, owing to new-generation synchrotron X-ray facilities and sensitive detectors, protein cry...
13 CitationsSource
#1Debsindhu Bhowmik (WSU: Wayne State University)H-Index: 9
#2Gurpreet K. Dhindsa (WSU: Wayne State University)H-Index: 3
Last.Xiang-qiang Chu (WSU: Wayne State University)H-Index: 13
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Life is traditionally seen as being driven by energy from the sun, however deep sea organisms have no access to sunlight, so they depend on nutrients found in the dusty chemical deposits and hydrothermal fluids around the hydrothermal vent zones, where they live. In this study, we use neutron spin-echo spectroscopy (NSE) to measure the inter-domain motions of the inorganic pyrophosphatase (IPPase) enzyme derived from thermostable microorganisms. IPPase is of extreme interest for biophysical stud...
5 CitationsSource
#1Joseph D. NgH-Index: 13
#2R.C. HughesH-Index: 5
Last.Andreas OstermannH-Index: 18
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