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Tomomi Aida
Tokyo Medical and Dental University
31Publications
11H-index
549Citations
Publications 31
Newest
#1Mizuki Uchida (Meijo University)H-Index: 1
#2Hirotake Hida (Meijo University)H-Index: 4
Last.Norio Ozaki (Nagoya University)H-Index: 56
view all 11 authors...
Abstract Alterations of the glutamatergic system components, including N -methyl- d -aspartate (NMDA) receptors are relevant to the pathophysiology of schizophrenia. Repeated phencyclidine (PCP) administration induces several schizophrenia-like psychobehavioral abnormalities and decreases extracellular glutamate levels, which are associated with increased levels of glial glutamate and aspartate transporter (GLAST) in the prefrontal cortex (PFC) of mice. In the present study, we investigated the ...
#1Yoshiki Miyasaka (Osaka University)H-Index: 2
#2Yoshihiro Uno (Osaka University)H-Index: 23
Last.Takao TanakaH-Index: 1
view all 15 authors...
CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats. The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allo...
#1Shota Nakade (Hiroshima University)H-Index: 6
#2Keiji Mochida (Hiroshima University)H-Index: 2
Last.Takashi Yamamoto (Hiroshima University)H-Index: 43
view all 9 authors...
Selective genome editing such as gene knock-in has recently been achieved by administration of chemical enhancer or inhibitor of particular DNA double-strand break (DSB) repair pathways, as well as overexpression of pathway-specific genes. In this study, we attempt to enhance the efficiency further to secure robust gene knock-ins, by using the local accumulation of DSB repair molecules (LoAD) system. We identify CtIP as a strong enhancer of microhomology-mediated end-joining (MMEJ) repair by gen...
#1Yuki Mizuno (Tokyo Medical and Dental University)H-Index: 2
#2Shu Shimada (Tokyo Medical and Dental University)H-Index: 10
Last.Atsushi Kudo (Tokyo Medical and Dental University)H-Index: 18
view all 14 authors...
Decrease in blood concentration of branched-chain amino acids, especially leucine, is known to promote liver carcinogenesis in patients with chronic liver disease, but the mechanism is unclear. We herein established hepatocellular carcinoma (HCC) cells knocked out for DEPDC5 by using the CRISPR/Cas9 system, and elucidated that cell viability of the DEPDC5 knockout (DEPDC5-KO) cells was higher than that of the DEPDC5 wild-type (DEPDC5-WT) under leucine starvation. Considering that autophagy defic...
#1Bettina Reichenbach (KI: Karolinska Institutet)H-Index: 1
#2Johanna Classon (KI: Karolinska Institutet)H-Index: 2
Last.Christian Göritz (KI: Karolinska Institutet)H-Index: 16
view all 6 authors...
Abstract Tissues contain distinct stem cell niches, but whether cell turnover is coordinated between niches during growth is unknown. Here, we report that in mouse skin, hair growth is accompanied by sebaceous gland and interfollicular epidermis expansion. During hair growth, cells in the bulge and outer root sheath temporarily upregulate the glutamate transporter SLC1A3, and the number of SLC1A3 + basal cells in interfollicular epidermis and sebaceous gland increases. Fate mapping of SLC1A3 + c...
#1Rolen M. Quadros (UNMC: University of Nebraska Medical Center)H-Index: 8
#2Hiromi Miura (Tokai University)H-Index: 10
Last.Daisuke Sakai (Tokai University)H-Index: 1
view all 22 authors...
Background Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homologous recombination in embryonic stem cells and are available for only ~25% of all mouse genes. Transgenic mice generated by random genomic insertion approaches pose problems of unreliable expression, and thu...
#1Kaori Sugiyama (Tokyo Medical and Dental University)H-Index: 2
#2Tomomi Aida (Tokyo Medical and Dental University)H-Index: 11
Last.Kohichi Tanaka (Tokyo Medical and Dental University)H-Index: 51
view all 6 authors...
Glutamate-mediated excitotoxicity induces neuronal death by altering various intracellular signaling pathways and is implicated as a common pathogenic pathway in many neurodegenerative diseases. In the case of motor neuron disease, there is significant evidence to suggest that overactivation of AMPA receptors due to deficiencies in the expression and function of glial glutamate transporter GLT1 and GLAST plays an important role in the mechanisms of neuronal death. However, a causal role for glia...
#1Yuri Kasagi (Tokyo Medical and Dental University)H-Index: 4
#2Daiei Takahashi (Tokyo Medical and Dental University)H-Index: 8
Last.Tatemitsu Rai (Tokyo Medical and Dental University)H-Index: 28
view all 12 authors...
Abstract Mutations in the with-no-lysine kinase 1 ( WNK1 ), WNK4 , Kelch-like 3 ( KLHL3 ), and Cullin3 ( CUL3 ) genes were identified as being responsible for hereditary hypertensive disease pseudohypoaldosteronism type II (PHAII). Normally, the KLHL3/CUL3 ubiquitin ligase complex degrades WNKs. In PHAII, the loss of interaction between KLHL3 and WNK4 increases levels of WNKs because of impaired ubiquitination, leading to abnormal over-activation of the WNK-OSR1/SPAK-NCC cascade in the kidney's ...
#1Tomomi Aida (Tokyo Medical and Dental University)H-Index: 11
#2Shota Nakade (Hiroshima University)H-Index: 6
Last.Takashi Yamamoto (Hiroshima University)H-Index: 43
view all 11 authors...
Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without long homology arms mediated by microhomology-mediated end-joining. Here, we identified exonuclease 1 (Exo1) as an enhancer for PITCh in human cells. By combining the Exo1 and PITCh-dire...
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