Tomomi Aida
Tokyo Medical and Dental University
31Publications
11H-index
507Citations
Publications 31
Newest
Published on Dec 1, 2018in Scientific Reports 4.12
Yuki Mizuno2
Estimated H-index: 2
(Tokyo Medical and Dental University),
Shu Shimada10
Estimated H-index: 10
(Tokyo Medical and Dental University)
+ 11 AuthorsAtsushi Kudo18
Estimated H-index: 18
(Tokyo Medical and Dental University)
Decrease in blood concentration of branched-chain amino acids, especially leucine, is known to promote liver carcinogenesis in patients with chronic liver disease, but the mechanism is unclear. We herein established hepatocellular carcinoma (HCC) cells knocked out for DEPDC5 by using the CRISPR/Cas9 system, and elucidated that cell viability of the DEPDC5 knockout (DEPDC5-KO) cells was higher than that of the DEPDC5 wild-type (DEPDC5-WT) under leucine starvation. Considering that autophagy defic...
1 Citations Source Cite
Published on Jul 11, 2018in bioRxiv
Jonathan J Wilde1
Estimated H-index: 1
(Massachusetts Institute of Technology),
Tomomi Aida11
Estimated H-index: 11
(Massachusetts Institute of Technology)
+ 3 AuthorsGuoping Feng1
Estimated H-index: 1
(Massachusetts Institute of Technology)
Recent advances in CRISPR/Cas-based genome editing, including three-component CRISPR, the use of long single-strand donors, enhanced microhomology-mediated end-joining (MMEJ), and pharmacological approaches have greatly improved knock-in (KI) efficiency. In our search for factors to further improve KI efficiency for broad therapeutic use and efficient generation of primate disease models, we found that the strand exchange protein RAD51 can significantly increase homozygous KI efficiency using CR...
4 Citations Source Cite
Published on Dec 1, 2018in BMC Genomics 3.73
Yoshiki Miyasaka1
Estimated H-index: 1
(Osaka University),
Yoshihiro Uno23
Estimated H-index: 23
(Osaka University)
+ 12 AuthorsTakao Tanaka1
Estimated H-index: 1
CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats. The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allo...
4 Citations Source Cite
Published on May 2, 2018in The EMBO Journal 10.56
Bettina Reichenbach1
Estimated H-index: 1
(Karolinska Institutet),
Johanna Classon2
Estimated H-index: 2
(Karolinska Institutet)
+ 3 AuthorsChristian Göritz16
Estimated H-index: 16
(Karolinska Institutet)
Abstract Tissues contain distinct stem cell niches, but whether cell turnover is coordinated between niches during growth is unknown. Here, we report that in mouse skin, hair growth is accompanied by sebaceous gland and interfollicular epidermis expansion. During hair growth, cells in the bulge and outer root sheath temporarily upregulate the glutamate transporter SLC1A3, and the number of SLC1A3 + basal cells in interfollicular epidermis and sebaceous gland increases. Fate mapping of SLC1A3 + c...
3 Citations Source Cite
Published on Dec 1, 2018in Nature Communications 12.35
Shota Nakade6
Estimated H-index: 6
(Hiroshima University),
Keiji Mochida2
Estimated H-index: 2
(Hiroshima University)
+ 6 AuthorsTakashi Yamamoto38
Estimated H-index: 38
(Hiroshima University)
Selective genome editing such as gene knock-in has recently been achieved by administration of chemical enhancer or inhibitor of particular DNA double-strand break (DSB) repair pathways, as well as overexpression of pathway-specific genes. In this study, we attempt to enhance the efficiency further to secure robust gene knock-ins, by using the local accumulation of DSB repair molecules (LoAD) system. We identify CtIP as a strong enhancer of microhomology-mediated end-joining (MMEJ) repair by gen...
1 Citations Source Cite
Published on Dec 1, 2017in Genome Biology 13.21
Rolen M. Quadros8
Estimated H-index: 8
(University of Nebraska Medical Center),
Hiromi Miura9
Estimated H-index: 9
(Tokai University)
+ 19 AuthorsDaisuke Sakai33
Estimated H-index: 33
(Tokai University)
Background Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homologous recombination in embryonic stem cells and are available for only ~25% of all mouse genes. Transgenic mice generated by random genomic insertion approaches pose problems of unreliable expression, and thu...
52 Citations Source Cite
Yuri Kasagi4
Estimated H-index: 4
(Tokyo Medical and Dental University),
Daiei Takahashi8
Estimated H-index: 8
(Tokyo Medical and Dental University)
+ 9 AuthorsTatemitsu Rai28
Estimated H-index: 28
(Tokyo Medical and Dental University)
Abstract Mutations in the with-no-lysine kinase 1 ( WNK1 ), WNK4 , Kelch-like 3 ( KLHL3 ), and Cullin3 ( CUL3 ) genes were identified as being responsible for hereditary hypertensive disease pseudohypoaldosteronism type II (PHAII). Normally, the KLHL3/CUL3 ubiquitin ligase complex degrades WNKs. In PHAII, the loss of interaction between KLHL3 and WNK4 increases levels of WNKs because of impaired ubiquitination, leading to abnormal over-activation of the WNK-OSR1/SPAK-NCC cascade in the kidney's ...
3 Citations Source Cite
Published on Sep 6, 2017in The Journal of Neuroscience 5.97
Kaori Sugiyama2
Estimated H-index: 2
(Tokyo Medical and Dental University),
Tomomi Aida11
Estimated H-index: 11
(Tokyo Medical and Dental University)
+ 3 AuthorsKohichi Tanaka49
Estimated H-index: 49
(Tokyo Medical and Dental University)
Glutamate-mediated excitotoxicity induces neuronal death by altering various intracellular signaling pathways and is implicated as a common pathogenic pathway in many neurodegenerative diseases. In the case of motor neuron disease, there is significant evidence to suggest that overactivation of AMPA receptors due to deficiencies in the expression and function of glial glutamate transporter GLT1 and GLAST plays an important role in the mechanisms of neuronal death. However, a causal role for glia...
6 Citations Source Cite
Published on Feb 1, 2016in Seikagaku
Tomomi Aida11
Estimated H-index: 11
,
Kohichi Tanaka49
Estimated H-index: 49
Published on Dec 1, 2016in BMC Genomics 3.73
Tomomi Aida11
Estimated H-index: 11
(Tokyo Medical and Dental University),
Shota Nakade6
Estimated H-index: 6
(Hiroshima University)
+ 8 AuthorsTakashi Yamamoto38
Estimated H-index: 38
(Hiroshima University)
Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without long homology arms mediated by microhomology-mediated end-joining. Here, we identified exonuclease 1 (Exo1) as an enhancer for PITCh in human cells. By combining the Exo1 and PITCh-dire...
18 Citations Source Cite
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