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Sangsu Bae
Hanyang University
54Publications
15H-index
2,535Citations
Publications 53
Newest
#1You Kyeong Jeong (Hanyang University)H-Index: 1
#2Jihyeon Yu (Hanyang University)H-Index: 6
Last.Sangsu Bae (Hanyang University)H-Index: 15
view all 3 authors...
Molecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an alternative to restriction enzymes. Here, by combining DNA cleavage via a Cas9 endonuclease and DNA ligation via Gibson assembly, we demonstrate a precise and practical DNA cloning method for replacin...
1 CitationsSource
#1Heon Seok KimH-Index: 6
#2You Kyeong Jeong (Hanyang University)H-Index: 1
Last.Sangsu Bae (Hanyang University)H-Index: 15
view all 5 authors...
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5–7) and in a confined TC*N sequence context. Adenine base editor–induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-speci...
1 CitationsSource
#1Choongil Lee (SNU: Seoul National University)H-Index: 5
#2Dong Hyun Jo (Seoul National University Hospital)H-Index: 13
Last.Sangsu Bae (Hanyang University)H-Index: 15
view all 9 authors...
A nonsense mutation is a substitutive mutation in a DNA sequence that causes a premature termination during translation and produces stalled proteins, resulting in dysfunction of a gene. Although it usually induces severe genetic disorders, there are no definite methods for inducing read through of premature termination codons (PTCs). Here, we present a targeted tool for bypassing PTCs, named CRISPR-pass, that uses CRISPR-mediated adenine base editors. CRISPR-pass, which should be applicable to ...
2 CitationsSource
#1Taeyoung KooH-Index: 15
#2A-Rum Yoon (Hanyang University)H-Index: 7
Last.Chae-Ok Yun (Hanyang University)H-Index: 42
view all 5 authors...
Approximately 15% of non-small cell lung cancer patients possesses several mutations in the epidermal growth factor receptor (EGFR) gene, which plays a critical role in tumor progression. To this end, we demonstrate that an adenovirus-mediated co-delivery of Cas9 and a guide RNA targeting oncogenic EGFR mutation commonly found in lung cancer can selectively remove oncogenic allele while being inactive against wild-type EGFR allele. An EGFR mutant harboring a single-nucleotide missense mutation (...
Source
#1Marion Gruffaz (SC: University of Southern California)H-Index: 1
#2Hongfeng Yuan (SC: University of Southern California)H-Index: 6
Last.Shou-Jiang GaoH-Index: 41
view all 9 authors...
ABSTRACT The abnormal proliferation of cancer cells is driven by deregulated oncogenes or tumor suppressors, among which the cancer-vulnerable genes are attractive therapeutic targets. Targeting mislocalization of oncogenes and tumor suppressors resulting from aberrant nuclear export is effective for inhibiting growth transformation of cancer cells. We performed a clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) screening in a unique model of matched primary and...
Source
#1Marion Gruffaz (SC: University of Southern California)H-Index: 1
#2Hongfeng Yuan (SC: University of Southern California)H-Index: 6
Last.Shou-Jiang Gao (University of Pittsburgh)H-Index: 1
view all 9 authors...
Abstract The abnormal proliferation of cancer cells is driven by deregulated oncogenes or tumor suppressors, of which the cancer vulnerable genes are attractive therapeutic targets. Targeting mislocalization of oncogenes and tumor suppressors resulting from aberrant nuclear export is effective for inhibiting growth transformation of cancer cells. We performed a CRISPR-Cas9 screening in a unique model of matched primary and oncogenic KSHV-transformed cells, and identified genes that were pro-grow...
Source
#1Yongmoon JeonH-Index: 6
#2You Hee ChoiH-Index: 1
Last.Sangsu BaeH-Index: 15
view all 7 authors...
Source
#1Keun-Tae Kim (Sogang University)H-Index: 2
#2Ju-Chan Park (Sogang University)H-Index: 1
Last.Hyuk-Jin Cha (SNU: Seoul National University)H-Index: 9
view all 10 authors...
An efficient gene editing technique for use in human pluripotent stem cells (hPSCs) would have great potential value in regenerative medicine, as well as in drug discovery based on isogenic human disease models. However, the extremely low efficiency of gene editing in hPSCs is a major technical hurdle that remains to be resolved. Previously, we demonstrated that YM155, a survivin inhibitor developed as an anti-cancer drug, induces highly selective cell death in undifferentiated hPSCs. In this st...
1 CitationsSource
#1Duck-Hyun Kim (SKKU: Sungkyunkwan University)H-Index: 9
#2Jihyeon Yu (Hanyang University)H-Index: 6
Last.Jehee Lee (SKKU: Sungkyunkwan University)H-Index: 46
view all 6 authors...
The CRISPR-Cas9 system has revolutionized genetic engineering and has been applied in numerous model organisms to date. To examine the capacity of the CRISPR-Cas9 system for generation of mutants in the marine rotifer Brachionus koreanus, we electroporated purified Cas9 proteins fused with GFP (Cas9-GFP) into rotifers. A dose-dependent increase in green fluorescent signal was highly detected in ovary and eggs. The purified Cas9-GFP proteins showed sustained fluorescence signals in rotifers at 24...
1 CitationsSource
#1Sangsu Bae (Hanyang University)H-Index: 15
#2Jinsoo Kim (SNU: Seoul National University)H-Index: 47
A machine-learning algorithm reliably predicts Cas9-edited genotypes arising from the repair of DNA double-strand breaks in mouse cells and human cells.
1 CitationsSource
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