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Sangsu Bae
Hanyang University
52Publications
13H-index
2,139Citations
Publications 52
Newest
Published on Mar 20, 2019in Scientific Reports 4.12
You Kyeong Jeong1
Estimated H-index: 1
(Hanyang University),
Jihyeon Yu7
Estimated H-index: 7
(Hanyang University),
Sangsu Bae13
Estimated H-index: 13
(Hanyang University)
Molecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an alternative to restriction enzymes. Here, by combining DNA cleavage via a Cas9 endonuclease and DNA ligation via Gibson assembly, we demonstrate a precise and practical DNA cloning method for replacin...
1 Citations Source Cite
Published on May 14, 2019in Mbio 6.69
Marion Gruffaz1
Estimated H-index: 1
,
Hongfeng Yuan6
Estimated H-index: 6
+ 6 AuthorsShou-Jiang Gao39
Estimated H-index: 39
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Published on Apr 6, 2019in bioRxiv
Marion Gruffaz1
Estimated H-index: 1
(SC: University of Southern California),
Hongfeng Yuan6
Estimated H-index: 6
(SC: University of Southern California)
+ 6 AuthorsShou-Jiang Gao (University of Pittsburgh)
The abnormal proliferation of cancer cells is driven by deregulated oncogenes or tumor suppressors, of which the cancer vulnerable genes are attractive therapeutic targets. Targeting mislocalization of oncogenes and tumor suppressors resulting from aberrant nuclear export is effective for inhibiting growth transformation of cancer cells. We performed a CRISPR-Cas9 screening in a unique model of matched primary and oncogenic KSHV-transformed cells, and identified genes that were pro-growth and gr...
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Published on Feb 1, 2019in Biophysical Journal 3.50
Yongmoon Jeon6
Estimated H-index: 6
,
You Hee Choi1
Estimated H-index: 1
+ 4 AuthorsSangsu Bae13
Estimated H-index: 13
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Published on Jan 16, 2019in bioRxiv
Keun-Tae Kim1
Estimated H-index: 1
(Sogang University),
Ju-Chan Park1
Estimated H-index: 1
(Sogang University)
+ 7 AuthorsHyuk-Jin Cha15
Estimated H-index: 15
(SNU: Seoul National University)
An efficient gene editing technique for use in human pluripotent stem cells (hPSCs) would have great potential value in regenerative medicine, as well as in drug discovery based on isogenic human disease models. However, the extremely low efficiency of gene editing in hPSCs is a major technical hurdle that remains to be resolved. Previously, we demonstrated that YM155, a survivin inhibitor developed as an anti-cancer drug, induces highly selective cell death in undifferentiated hPSCs. In this st...
1 Citations Source Cite
Published on Dec 14, 2018in Hydrobiologia 2.17
Duck-Hyun Kim9
Estimated H-index: 9
(SKKU: Sungkyunkwan University),
Jihyeon Yu7
Estimated H-index: 7
(Hanyang University)
+ 3 AuthorsJehee Lee49
Estimated H-index: 49
(SKKU: Sungkyunkwan University)
The CRISPR-Cas9 system has revolutionized genetic engineering and has been applied in numerous model organisms to date. To examine the capacity of the CRISPR-Cas9 system for generation of mutants in the marine rotifer Brachionus koreanus, we electroporated purified Cas9 proteins fused with GFP (Cas9-GFP) into rotifers. A dose-dependent increase in green fluorescent signal was highly detected in ovary and eggs. The purified Cas9-GFP proteins showed sustained fluorescence signals in rotifers at 24...
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Published on Dec 1, 2018in Nature Biomedical Engineering
Sangsu Bae13
Estimated H-index: 13
(Hanyang University),
Jinsoo Kim49
Estimated H-index: 49
(SNU: Seoul National University)
A machine-learning algorithm reliably predicts Cas9-edited genotypes arising from the repair of DNA double-strand breaks in mouse cells and human cells.
1 Citations Source Cite
Published on Dec 1, 2018in Nature Communications 12.35
Jung-Hee Lee4
Estimated H-index: 4
(Chosun University),
Seon-Joo Park3
Estimated H-index: 3
(Chosun University)
+ 9 AuthorsJihyeon Yu7
Estimated H-index: 7
(Hanyang University)
This Article contains errors in Fig. 3, Fig. 4 and Fig. 7, for which we apologize. In Fig. 3, panel ‘b’, the 0.5 hour time point after Ku55933 treatment images were inadvertently replaced with duplicates of the 3 hour time point after Ku55933 treatment images in Fig. 3b. Additionally, in panel ‘b’, the 0.5 hour time point after Nu7026 treatment images were inadvertently replaced with duplicates of the 180 min time point after siMDC1 treatment images in Fig. 3d. In Fig. 4, panel ‘g’, RNF168 foci ...
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Published on Dec 1, 2018in Nature Communications 12.35
Yongmoon Jeon6
Estimated H-index: 6
(KIST: Korea Institute of Science and Technology),
You Hee Choi1
Estimated H-index: 1
(Gwangju Institute of Science and Technology)
+ 10 AuthorsJin-Soo Kim52
Estimated H-index: 52
(SNU: Seoul National University)
Cas12a (also called Cpf1) is a representative type V-A CRISPR effector RNA-guided DNA endonuclease, which provides an alternative to type II CRISPR–Cas9 for genome editing. Previous studies have revealed that Cas12a has unique features distinct from Cas9, but the detailed mechanisms of target searching and DNA cleavage by Cas12a are still unclear. Here, we directly observe this entire process by using single-molecule fluorescence assays to study Cas12a from Acidaminococcus sp. (AsCas12a). We det...
8 Citations Source Cite
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