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Sangsu Bae
Hanyang University
54Publications
13H-index
2,141Citations
Publications 54
Newest
Published on Mar 20, 2019in Scientific Reports4.01
You Kyeong Jeong1
Estimated H-index: 1
(Hanyang University),
Jihyeon Yu7
Estimated H-index: 7
(Hanyang University),
Sangsu Bae13
Estimated H-index: 13
(Hanyang University)
Molecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an alternative to restriction enzymes. Here, by combining DNA cleavage via a Cas9 endonuclease and DNA ligation via Gibson assembly, we demonstrate a precise and practical DNA cloning method for replacin...
Published on Sep 23, 2019in Nature Biotechnology31.86
Heon Seok Kim6
Estimated H-index: 6
,
You Kyeong Jeong1
Estimated H-index: 1
(Hanyang University)
+ 2 AuthorsSangsu Bae13
Estimated H-index: 13
(Hanyang University)
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5–7) and in a confined TC*N sequence context. Adenine base editor–induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-speci...
Published on 2019in Molecular Therapy8.40
Choongil Lee5
Estimated H-index: 5
(SNU: Seoul National University),
Dong Hyun Jo12
Estimated H-index: 12
(Seoul National University Hospital)
+ 5 AuthorsSangsu Bae13
Estimated H-index: 13
(Hanyang University)
A nonsense mutation is a substitutive mutation in a DNA sequence that causes a premature termination during translation and produces stalled proteins, resulting in dysfunction of a gene. Although it usually induces severe genetic disorders, there are no definite methods for inducing read through of premature termination codons (PTCs). Here, we present a targeted tool for bypassing PTCs, named CRISPR-pass, that uses CRISPR-mediated adenine base editors. CRISPR-pass, which should be applicable to ...
Published on Jul 1, 2019
Taeyoung Koo14
Estimated H-index: 14
,
A-Rum Yoon13
Estimated H-index: 13
+ 2 AuthorsChae-Ok Yun40
Estimated H-index: 40
Published on May 14, 2019in Mbio6.75
Marion Gruffaz1
Estimated H-index: 1
,
Hongfeng Yuan6
Estimated H-index: 6
+ 6 AuthorsShou-Jiang Gao39
Estimated H-index: 39
ABSTRACT The abnormal proliferation of cancer cells is driven by deregulated oncogenes or tumor suppressors, among which the cancer-vulnerable genes are attractive therapeutic targets. Targeting mislocalization of oncogenes and tumor suppressors resulting from aberrant nuclear export is effective for inhibiting growth transformation of cancer cells. We performed a clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) screening in a unique model of matched primary and...
Published on Apr 6, 2019in bioRxiv
Marion Gruffaz1
Estimated H-index: 1
(SC: University of Southern California),
Hongfeng Yuan6
Estimated H-index: 6
(SC: University of Southern California)
+ 6 AuthorsShou-Jiang Gao (University of Pittsburgh)
Abstract The abnormal proliferation of cancer cells is driven by deregulated oncogenes or tumor suppressors, of which the cancer vulnerable genes are attractive therapeutic targets. Targeting mislocalization of oncogenes and tumor suppressors resulting from aberrant nuclear export is effective for inhibiting growth transformation of cancer cells. We performed a CRISPR-Cas9 screening in a unique model of matched primary and oncogenic KSHV-transformed cells, and identified genes that were pro-grow...
Published on Feb 1, 2019in Biophysical Journal3.67
Yongmoon Jeon6
Estimated H-index: 6
,
You Hee Choi1
Estimated H-index: 1
+ 4 AuthorsSangsu Bae13
Estimated H-index: 13
Published on Jan 16, 2019in bioRxiv
Keun-Tae Kim1
Estimated H-index: 1
(Sogang University),
Ju-Chan Park1
Estimated H-index: 1
(Sogang University)
+ 7 AuthorsHyuk-Jin Cha15
Estimated H-index: 15
(SNU: Seoul National University)
An efficient gene editing technique for use in human pluripotent stem cells (hPSCs) would have great potential value in regenerative medicine, as well as in drug discovery based on isogenic human disease models. However, the extremely low efficiency of gene editing in hPSCs is a major technical hurdle that remains to be resolved. Previously, we demonstrated that YM155, a survivin inhibitor developed as an anti-cancer drug, induces highly selective cell death in undifferentiated hPSCs. In this st...
Published on Dec 14, 2018in Hydrobiologia2.33
Duck-Hyun Kim9
Estimated H-index: 9
(SKKU: Sungkyunkwan University),
Jihyeon Yu7
Estimated H-index: 7
(Hanyang University)
+ 3 AuthorsJehee Lee47
Estimated H-index: 47
(SKKU: Sungkyunkwan University)
The CRISPR-Cas9 system has revolutionized genetic engineering and has been applied in numerous model organisms to date. To examine the capacity of the CRISPR-Cas9 system for generation of mutants in the marine rotifer Brachionus koreanus, we electroporated purified Cas9 proteins fused with GFP (Cas9-GFP) into rotifers. A dose-dependent increase in green fluorescent signal was highly detected in ovary and eggs. The purified Cas9-GFP proteins showed sustained fluorescence signals in rotifers at 24...
Published on Dec 1, 2018in Nature Biomedical Engineering
Sangsu Bae13
Estimated H-index: 13
(Hanyang University),
Jinsoo Kim49
Estimated H-index: 49
(SNU: Seoul National University)
A machine-learning algorithm reliably predicts Cas9-edited genotypes arising from the repair of DNA double-strand breaks in mouse cells and human cells.
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