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Ram Sarup Singh
Punjabi University
ChemistryLectinBiochemistryBiologyMicrobiology
160Publications
29H-index
3,163Citations
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Publications 158
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#1Ram Sarup Singh (Punjabi University)H-Index: 29
#2T.N. Singh (Punjabi University)H-Index: 6
Last. AshokPandey (CSIR: Council of Scientific and Industrial Research)H-Index: 77
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Abstract In present study, statistical optimization of medium composition for endoinulinase production from raw inulin using Aspergillus tritici was performed in shake-flask fermentations. The concentration of four independent variables viz. raw inulin (1.5–3.5%), peptone (0.2–1.8%), (NH4)H2PO4 (0.2–1.8%) and pH (4.5–6.5) was explored to optimize the composition of fermentation medium for maximum endoinulinase production. Fermentation medium containing 2.5% raw inulin, 1% peptone, 1% (NH4)H2PO4 ...
1 CitationsSource
#1Ram Sarup Singh (Punjabi University)H-Index: 29
#2Amandeep Kaur Walia (Punjabi University)H-Index: 6
Last. John F. KennedyH-Index: 69
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Abstract Lectins are unique biorecognition proteins which recognize and interact with various cell surface carbohydrates/glycoproteins. These ubiquitous molecules are involved in various cell-cell interactions and can be exploited to analyze cell surface associated interactions and biological functions. Amongst fungi, lectins have been extensively explored from mushrooms as compared to microfungi or yeasts. Lectins from basidiomycetes have diverse features in terms of their physico-chemical char...
1 CitationsSource
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Abstract A xylose-specific intracellular lectin, showing hemagglutination only with rabbit erythrocytes was purified from mycelium of Fusarium sambucinum which was designated as FSL. An array of anion exchange chromatography on Q-Sepharose and gel-exclusion chromatography on Sephadex G-100 resulted in 84.21% yield and 53.99-fold purification of lectin with specific activity of 169.53 titre/mg. Molecular weight of FSL determined by SDS-PAGE was 70.7 kDa, which was further confirmed by gel-exclusi...
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#1Ram Sarup Singh (Punjabi University)H-Index: 29
#2Kanika Chauhan (Punjabi University)H-Index: 9
Last. AshokPandey (IITR: Indian Institute of Toxicology Research)H-Index: 77
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Abstract The present work was designed to determine the suitability of apple pomace for inulinase production from Mucor circinelloides by solid-state fermentation (SSF). Central composite rotatable deign (CCRD) matrix (15 runs) of RSM was experimentally executed to investigate the influence of moisture, pH and fermentation time on fungal inulinase production from apple pomace by SSF. SSFs were performed at flask-level at 30 °C. Under optimized conditions viz., moisture 83.5%, pH 6.4 and fermenta...
1 CitationsSource
#1Ram Sarup Singh (Punjabi University)H-Index: 29
#2Navpreet Kaur (Punjabi University)H-Index: 16
Last. John F. KennedyH-Index: 69
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Abstract The synthesis of carbamoylethyl locust bean gum (CLBG) was optimized using Plackett-Burman design. The generated model showed high significance (p
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#1Ram Sarup Singh (Punjabi University)H-Index: 29
#2Kanika Chauhan (Punjabi University)H-Index: 9
Last. John F. KennedyH-Index: 69
view all 3 authors...
Abstract The present study describes the response surface optimization (RSM) of inulinase immobilization on carbon nanofibers (CNFs), modified using 3-aminopropyl-triethoxysilane (APTES) and glutaraldehyde (GA). Immobilized protein content and inulinase activity were used as indicators of immobilization. Under RSM optimised conditions viz., sonication time 120 min, APTES concentration 3.0%, GA concentration 2.0%, GA activation time 75 min, enzyme load 27.5 IU and enzyme coupling time 2.75 h, inu...
2 CitationsSource
#1Ram Sarup Singh (Punjabi University)H-Index: 29
#2Kanika Chauhan (Punjabi University)H-Index: 9
Abstract A simple strategy for enzyme immobilization onto homo- and hetero-functionalized multiwalled carbon nanotubes (MWCNTs) is described. Homo-functionalization of MWCNTs can be carried out using an amino-silane compound, i.e., 3-aminopropyl-triethoxysilane (APTES). It is an important silane coupling agent, which promotes interfacial behavior of nanomaterials. Whereas, hetero-functionalization of MWCNTs can be performed using APTES and cross-linking agent glutaraldehyde (GA). Key parameters ...
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#1Ram Sarup Singh (Punjabi University)H-Index: 29
#2Navpreet Kaur (Punjabi University)H-Index: 16
Last. John F. KennedyH-Index: 69
view all 4 authors...
Abstract In the present investigation, polyethylene glycol (PEG 6000) was used for downstream processing of pullulan from Aureobasidium pullulans by aqueous phase separation (APS) technique. The cell-free broth was processed with PEG solution (10–35%, w/w) and pullulan formed a clear separate phase with all concentrations of PEG i.e. pullulan in lower phase and PEG solution along with impurities in upper phase. Maximum pullulan recovery from cell-free broth was obtained by PEG 25% (w/w). The sam...
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#1Ram Sarup Singh (Punjabi University)H-Index: 29
#2Kanika Chauhan (Punjabi University)H-Index: 9
Last. Ashok Pandey (IITR: Indian Institute of Toxicology Research)H-Index: 12
view all 3 authors...
Abstract In present study, inulinase production was carried out from paneer whey using Penicillium oxalicum BGPUP-4 in a stirred tank bioreactor. In preliminary experimentation in shake-flask fermentations, maximum (38.58 IU/mL) inulinase production was obtained from clarified paneer whey. Therefore, it was selected for inulinase production at bioreactor level. Probabilistic tool was used to optimise process variables for inulinase production in bioreactor. At optimal combination of process vari...
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