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Dana Carroll
University of Utah
Homologous recombinationMolecular biologyGeneticsDNABiology
132Publications
43H-index
8,710Citations
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Publications 128
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#1Dana Carroll (UofU: University of Utah)H-Index: 43
This editorial discusses what levels of off-target effects can be tolerated in genome editing, in the context of various types of applications.
2 CitationsSource
#1Robert M. Yarrington (UofU: University of Utah)H-Index: 7
#2Surbhi Verma (UofU: University of Utah)H-Index: 6
Last. Dana Carroll (UofU: University of Utah)H-Index: 43
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Genome editing with CRISPR-Cas nucleases has been applied successfully to a wide range of cells and organisms. There is, however, considerable variation in the efficiency of cleavage and outcomes at different genomic targets, even within the same cell type. Some of this variability is likely due to the inherent quality of the interaction between the guide RNA and the target sequence, but some may also reflect the relative accessibility of the target. We investigated the influence of chromatin st...
27 CitationsSource
#1Fyodor D. UrnovH-Index: 46
#2Pamela C. Ronald (UC Davis: University of California, Davis)H-Index: 72
Last. Dana Carroll (UofU: University of Utah)H-Index: 43
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7 CitationsSource
The efficacy of the powerful CRISPR-Cas9 genome-editing platform depends on DNA repair activities in the cells being targeted. Two new papers show that the low efficiency of targeting in some primary human cell lines is the result of p53-dependent cell arrest in response to the Cas9-induced break. This limitation must be overcome for some anticipated therapies.
1 CitationsSource
6 Citations
#1Roberta Sarno (University of Paris)H-Index: 1
#2Yoan Vicq (University of Paris)H-Index: 1
Last. Alain Nicolas (University of Paris)H-Index: 34
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8 CitationsSource
#1Srinivasan Chandrasegaran (Johns Hopkins University)H-Index: 30
#2C. Korin Bullen (Johns Hopkins University)H-Index: 3
Last. Dana CarrollH-Index: 43
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#1Mark A. DeWitt (University of California, Berkeley)H-Index: 10
#2Jacob E. Corn (University of California, Berkeley)H-Index: 32
Last. Dana Carroll (University of California, Berkeley)H-Index: 43
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The CRISPR-Cas genome editing system is very powerful. The format of the CRISPR reagents and the means of delivery are often important factors in targeting efficiency. Delivery of recombinant Cas9 protein and guide RNA (gRNA) as a preformed ribonucleoprotein (RNP) complex has recently emerged as a powerful and general approach to genome editing. Here we outline methods to produce and deliver Cas9 RNPs. A donor DNA carrying desired sequence changes can also be included to program precise sequence...
33 CitationsSource
#1Dana Carroll (UofU: University of Utah)H-Index: 43
The development of the CRISPR-Cas platform for genome editing has greatly simplified the process of making targeted genetic modifications. Applications of genome editing are expected to have a substantial impact on human therapies through the development of better animal models, new target discovery, and direct therapeutic intervention.
10 CitationsSource
#2Marina O'ReillyH-Index: 7
Last. Feng ZhangH-Index: 115
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