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Weizhi Ji
Kunming University of Science and Technology
125Publications
25H-index
3,441Citations
Publications 123
Newest
#1Lifeng XiangH-Index: 1
#2Yu Yin (Kunming University of Science and Technology)H-Index: 1
Last.Tianqing Li (Kunming University of Science and Technology)H-Index: 9
view all 18 authors...
Knowledge detailing human pre-gastrulation embryonic development including spatial self-organization and cell type ontogeny remains limited by available two-dimensional technological platforms1,2 that do not recapitulate the in vivo condition3–5. Here, we report a three-dimensional (3D) blastocyst-culture system, which enables human blastocyst development through primitive streak anlage (PSA). These 3D embryos mimic in vivo developmental landmarks and 3D architectures, including embryonic disc, ...
1 CitationsSource
#1Bin Li (SU: Southern University and A&M College)
#2Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
Last.Yizhou Dong (OSU: Ohio State University)
view all 4 authors...
The CRISPR (clustered regularly interspaced short palindromic repeats)-based genome editing technology is an emerging RNA-guided nuclease system initially identified from the microbial adaptive immune systems. In recent years, the CRISPR system has been reprogrammed to target specific regions of the eukaryotic genome and has become a powerful tool for genetic engineering. Researchers have explored many approaches to improve the genome editing activity of the CRISPR–Cas system and deliver its com...
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#1Xin Luo (KIZ: Kunming Institute of Zoology)H-Index: 2
#2Yaoxi He (KIZ: Kunming Institute of Zoology)H-Index: 5
Last.Bing Su (KIZ: Kunming Institute of Zoology)H-Index: 23
view all 15 authors...
CRISPR-Cas9 is a widely-used genome editing tool, but its off-target effect and on-target complex mutations remain a concern, especially in view of future clinical applications. Non-human primates (NHPs) share close genetic and physiological similarities with humans, making them an ideal preclinical model for developing Cas9-based therapies. However, to our knowledge no comprehensive in vivo off-target and on-target assessment has been conducted in NHPs. Here, we perform whole genome trio sequen...
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#1Shanshan Li (Kunming University of Science and Technology)H-Index: 2
#2Lei Ao (Boston Children's Hospital)H-Index: 1
Last.Wei Si (Kunming University of Science and Technology)H-Index: 12
view all 12 authors...
Background Although sperm cryopreservation has been widely used in human reproductive medicine as an integral infertility management in infertility clinics and for banking sperm in sperm banks, the freezing/thawing protocols are not optimal. The freezing and thawing processes result in changes at both structural and molecular levels, some even detrimental, in human sperm when compared with fresh sperm. The change of sperm proteins after cryopreservation may play negative roles for fertilization ...
1 CitationsSource
#1Chu Chu (Kunming University of Science and Technology)H-Index: 1
#2Zhaohui Yang (Kunming University of Science and Technology)H-Index: 17
Last.Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
view all 10 authors...
Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system. It has been demonstrated that ...
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#1Yuyu Niu (Kunming University of Science and Technology)H-Index: 17
#2Nianqin Sun (Kunming University of Science and Technology)H-Index: 1
Last.Tao Tan (Kunming University of Science and Technology)H-Index: 10
view all 24 authors...
The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms underlying this transition. Here, we established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bi...
7 CitationsSource
#1Bo Zhang (Kunming University of Science and Technology)
#2Zhigang Zhou (Kunming University of Science and Technology)H-Index: 1
Last.Yongchang Chen (Kunming University of Science and Technology)H-Index: 13
view all 8 authors...
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#1Dan Wang (UMMS: University of Massachusetts Medical School)H-Index: 23
#2Dayong Wang (UMMS: University of Massachusetts Medical School)H-Index: 86
Last.Weizhi Ji (Kunming University of Science and Technology)H-Index: 25
view all 10 authors...
Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno‐associated viruses (rAAVs). Together with previous reports from the authors of this paper and...
1 CitationsSource
#1Shengnan Wang (Kunming University of Science and Technology)
#2Yanchao Duan (Kunming University of Science and Technology)H-Index: 3
Last.Wei Si (Kunming University of Science and Technology)H-Index: 12
view all 12 authors...
Abstract When nonhuman primate sperm undergoes cryopreservation in an egg yolk medium there is an increased risk that the egg yolk might adversely affect the sperm due to containing of avian pathogens. Although commercial egg-yolk-free medium for human sperm cryopreservation has been used for macaque sperm, the cryo-survival remains less than optimal. The present study, therefore, was conducted to determine the optimal concentration of antifreeze protein (AFP) III supplemented in a commercial eg...
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#1Jianguo Zhao (CAS: Chinese Academy of Sciences)
#2Liangxue Lai (Guangzhou Institutes of Biomedicine and Health)
Last.Qi Zhou (CAS: Chinese Academy of Sciences)H-Index: 54
view all 4 authors...
Large animals (non-human primates, livestock and dogs) are playing important roles in biomedical research, and large livestock animals serve as important sources of meat and milk. The recently developed programmable DNA nucleases have revolutionized the generation of gene-modified large animals that are used for biological and biomedical research. In this review, we briefly introduce the recent advances in nuclease-meditated gene editing tools, and we outline these editing tools applications in ...
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