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Aaron Greenwood
University of California, Riverside
31Publications
15H-index
948Citations
Publications 31
Newest
#1Tzu-Ping KoH-Index: 35
#2S. KoszelakH-Index: 12
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#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
#2Robert W. Lucas (UCI: University of California, Irvine)H-Index: 8
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
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Difference electron density maps, based on structure factor amplitudes and experimental phases from crystals of wild-type turnip yellow mosaic virus and those of empty capsids prepared by freeze-thawing, show a large portion of the encapsidated RNA to have an icosahedral distribution. Four unique segments of base-paired, double-helical RNA, one to two turns in length, lie between 33-A and 101-A radius and are organized about either 2-fold or 5-fold icosahedral axes. In addition, single-stranded ...
21 CitationsSource
#1Yurii G. Kuznetsov (UCI: University of California, Irvine)H-Index: 17
#2Shoibal Datta (UCI: University of California, Irvine)H-Index: 5
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
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Abstract NIH 3T3 cells were infected in culture with the oncogenic retrovirus, mouse leukemia virus (MuLV), and studied using atomic force microscopy (AFM). Cells fixed with glutaraldehyde alone, and those postfixed with osmium tetroxide, were imaged under ethanol according to procedures that largely preserved their structures. With glutaraldehyde fixation alone, the lipid bilayer was removed and maturing virions were seen emerging from the cytoskeletal matrix. With osmium tetroxide postfixation...
43 CitationsSource
#1Yurii G. Kuznetsov (UCI: University of California, Irvine)H-Index: 17
#2Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
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Abstract Satellite tobacco mosaic virus (STMV) can undergo at least two physical transitions that significantly alter its mechanical and structural characteristics. At high pH the 17-nm STMV particles expand radially by about 5 A to yield particles having diameters of about 18 nm. This pH-induced transition is further promoted by aging of the virions and degradation of the RNA, so that swollen particles ultimately appear even at neutral pH. While the native 17-nm particles crystallize as orthorh...
17 CitationsSource
#1John W. Day (UCI: University of California, Irvine)H-Index: 60
#2Yuri G. Kuznetsov (UCI: University of California, Irvine)H-Index: 6
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
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Satellite tobacco mosaic virus (STMV) was probed using a variety of proteases. Consequences of the degradation were analyzed using gel electrophoresis, quasi-elastic light scattering (QELS), and atomic force microscopy (AFM). Proteolysis rates of 30 minutes for complete degradation of the protein capsid, up to many hours, were investigated. With each protease, degradation of virions 17 nm in diameter was shown by QELS to result in particles of 10 nm diameter, which is that of the RNA core observ...
21 CitationsSource
#2John W. DayH-Index: 60
Last.Alexander McPhersonH-Index: 51
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#1Steven B. Larson (UCI: University of California, Irvine)H-Index: 22
#2John W. Day (UCI: University of California, Irvine)H-Index: 60
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
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Abstract Desmodium yellow mottle virus is a 28 nm diameter, T = 3 icosahedral plant virus of the tymovirus group. Its structure has been solved to a resolution of 2.7 A using X-ray diffraction analysis based on molecular replacement and phase extension methods. The final R value was 0.151 ( R free = 0.159) for 134,454 independent reflections. The folding of the polypeptide backbone is nearly identical with that of turnip yellow mosaic virus, as is the arrangement of subunits in the virus capsid....
23 CitationsSource
#1Joseph D. NgH-Index: 7
#2Bernard LorberH-Index: 28
Last.Alexander McPhersonH-Index: 2
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#1Steven B. Larson (UCR: University of California, Riverside)H-Index: 22
#2John W. Day (UCR: University of California, Riverside)H-Index: 60
Last.Alexander McPherson (UCR: University of California, Riverside)H-Index: 51
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Abstract The molecular structure of satellite tobacco mosaic virus (STMV) has been refined to 1.8 A resolution using X-ray diffraction data collected from crystals grown in microgravity. The final R value was 0.179 and R free was 0.184 for 219,086 independent reflections. The final model of the asymmetric unit contained amino acid residues 13 to 159 of a coat protein monomer, 21 nucleotides, a sulfate ion, and 168 water molecules. The nucleotides were visualized as 30 helical segments of nine ba...
100 CitationsSource
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