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Wei-Lin Ou
University of Toronto
11Publications
4H-index
28Citations
Publications 11
Newest
#1Takefumi Morizumi (U of T: University of Toronto)H-Index: 17
#2Wei-Lin Ou (U of T: University of Toronto)H-Index: 4
Last.Oliver P. Ernst (U of T: University of Toronto)H-Index: 34
view all 7 authors...
Gloeobacter rhodopsin (GR) is a cyanobacterial proton pump which can be potentially applied to optogenetics. We solved the crystal structure of GR and found that it has overall similarity to the homologous proton pump from Salinibacter ruber, xanthorhodopsin (XR). We identified distinct structural characteristics of GR’s hydrogen bonding network in the transmembrane domain as well as the displacement of extracellular sides of the transmembrane helices relative to those of XR. Employing Raman spe...
Source
Last.Wei-Lin OuH-Index: 4
view all 3 authors...
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#1Jana BroeckerH-Index: 10
#2Takefumi MorizumiH-Index: 17
Last.Oliver P. ErnstH-Index: 34
view all 13 authors...
This protocol describes procedures for the high-throughput in situ X-ray screening of and data collection from soluble and membrane-protein crystals at room temperature and under cryogenic conditions using Mylar-based film sandwich plates.
8 CitationsSource
#1Jana BroeckerH-Index: 10
#2Takefumi MorizumiH-Index: 17
Last.Oliver P. ErnstH-Index: 34
view all 4 authors...
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#1Jana BroeckerH-Index: 10
#2Takefumi MorizumiH-Index: 17
Last.Oliver P. ErnstH-Index: 34
view all 13 authors...
Source
#2Wei-Lin OuH-Index: 4
view all 3 authors...
Source
#1Alice Verchère (Cornell University)H-Index: 2
#2Wei-Lin Ou (U of T: University of Toronto)H-Index: 4
Last.Anant K. Menon (Cornell University)H-Index: 43
view all 9 authors...
The retinylidene protein bacteriorhodopsin (BR) is a heptahelical light-dependent proton pump found in the purple membrane of the archaeon Halobacterium salinarum. We now show that when reconstituted into large unilamellar vesicles, purified BR trimers exhibit light-independent lipid scramblase activity, thereby facilitating transbilayer exchange of phospholipids between the leaflets of the vesicle membrane at a rate >10,000 per trimer per second. This activity is comparable to that of recently ...
5 CitationsSource
#1Wei-Lin Ou (U of T: University of Toronto)H-Index: 4
#2Ned Van Eps (U of T: University of Toronto)H-Index: 19
Last.Oliver P. Ernst (U of T: University of Toronto)H-Index: 34
view all 5 authors...
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#1Jana BroeckerH-Index: 10
#2Viviane KlingelH-Index: 2
Last.Oliver P. ErnstH-Index: 34
view all 8 authors...
In recent years, in situ data collection has been a major focus of progress in protein crystallography. Here, we introduce the Mylar in situ method using Mylar-based sandwich plates that are inexpensive, easy to make and handle, and show significantly less background scattering than other setups. A variety of cognate holders for patches of Mylar in situ sandwich films corresponding to one or more wells makes the method robust and versatile, allows for storage and shipping of entire wells, and en...
10 CitationsSource
#1Kelly Ann Robinson (U of T: University of Toronto)H-Index: 1
#2Wei-Lin Ou (U of T: University of Toronto)H-Index: 4
Last.Jane Mitchell (U of T: University of Toronto)H-Index: 10
view all 7 authors...
Invertebrate visual opsins are G protein-coupled receptors coupled to retinoid chromophores that isomerize reversibly between inactive rhodopsin and active metarhodopsin upon absorption of photons of light. The squid visual system has an arrestin protein that binds to metarhodopsin to block signaling to Gq and activation of phospholipase C. Squid rhodopsin kinase (SQRK) can phosphorylate both metarhodopsin and arrestin, a dual role that is unique among the G protein-coupled receptor kinases. The...
5 CitationsSource
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