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Yurii G. Kuznetsov
University of California, Irvine
33Publications
16H-index
784Citations
Publications 33
Newest
#1Neil HoaH-Index: 16
#2Lisheng GeH-Index: 8
Last.Martin R. JadusH-Index: 22
view all 9 authors...
Cancer cells derived from Glioblastoma multiforme possess membranous protrusions allowing these cells to infiltrate surrounding tissue, while resisting lymphocyte cytotoxicity. Microvilli and filopodia are supported by actin filaments cross-linked by fascin. Fascin-1 was genetically silenced within human U251 glioma cells; these knock-down glioma cells lost their microvilli/filopodia. The doubling time of these fascin-1 knock-down cells was doubled that of shRNA control U251 cells. Fascin-1 knoc...
#1Neil HoaH-Index: 16
#2Lisheng GeH-Index: 8
view all 9 authors...
Cancer cells derived from Glioblastoma multiforme possess membranous protrusions allowing these cells to infiltrate surrounding tissue, while resisting lymphocyte cytotoxicity. Microvilli and filopodia are supported by actin filaments cross-linked by fascin. Fascin-1 was genetically silenced within human U251 glioma cells; these knock- down glioma cells lost their microvilli/filopodia. The doubling time of these fascin-1 knock-down cells was doubled that of shRNA control U251 cells. Fascin-1 kno...
#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
#2Yurii G. Kuznetsov (UCI: University of California, Irvine)H-Index: 16
The nucleation and growth of protein, nucleic acid and virus crystals from solution are functions of underlying kinetic and thermodynamic parameters that govern the process, and these are all supersaturation-dependent. While the mechanisms of macromolecular crystal growth are essentially the same as for conventional crystals, the underlying parameters are vastly different, in some cases orders of magnitude lower, and this produces very different crystallization processes. Numerous physical featu...
#1Yurii G. KuznetsovH-Index: 16
#2Sheng-Chieh ChangH-Index: 2
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
view all 4 authors...
The objective was to visualize a variety of marine bacteriophage and identify unique structural features that set them apart from terrestrial phages. Phages were plaque isolated and characterized using atomic force microscopy. Bacteriophage infecting cyanobacteria synechococcus that exhibits a novel structural feature not previously reported for any other phages were observed. These cyanophages have up to four, 450 nm long, multi-stranded, complex helical fibers that emanate from either the base...
#1Yurii G. Kuznetsov (UCI: University of California, Irvine)H-Index: 16
#2Sheng-Chieh Chang (UCI: University of California, Irvine)H-Index: 2
Last.Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
view all 5 authors...
Abstract Marine viruses have only relatively recently come to the attention of molecular biologists, and the extraordinary diversity of potential host organisms suggests a new wealth of genetic and structural forms. A promising technology for characterizing and describing the viruses structurally is atomic force microscopy (AFM). We provide examples here of some of the different architectures and novel structural features that emerge from even a very limited investigation, one focused on cyanoph...
If serum albumin from several animals, including humans, at concentrations greater than about 60 mg/mL is combined with an aqueous extract of porcine or human pancreas, then massive amounts of microcrystals develop. Both raw blood serum, as well as a range of concentrated, pure proteins, when combined with the extract produced identical crystals. Morphologies of crystals and their aggregates did, however, show some dependence on the starting protein material. Crystals could be dissolved by boili...
#1Yurii G. Kuznetsov (UCI: University of California, Irvine)H-Index: 16
#2Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
Summary: Atomic force microscopy (AFM) can visualize almost everything pertinent to structural virology and at resolutions that approach those for electron microscopy (EM). Membranes have been identified, RNA and DNA have been visualized, and large protein assemblies have been resolved into component substructures. Capsids of icosahedral viruses and the icosahedral capsids of enveloped viruses have been seen at high resolution, in some cases sufficiently high to deduce the arrangement of protein...
#1Kristina Clemens (UCI: University of California, Irvine)H-Index: 4
#2Liza S.Z. Larsen (UCI: University of California, Irvine)H-Index: 6
Last.Suzanne SandmeyerH-Index: 27
view all 12 authors...
Cells expressing the yeast retrotransposon Ty3 form concentrated foci of Ty3 proteins and RNA within which virus-like particle (VLP) assembly occurs. Gag3, the major structural protein of the Ty3 retrotransposon, is composed of capsid (CA), spacer (SP), and nucleocapsid (NC) domains analogous to retroviral domains. Unlike the known SP domains of retroviruses, Ty3 SP is highly acidic. The current studies investigated the role of this domain. Although deletion of Ty3 SP dramatically reduced retrot...
#1Yurii G. Kuznetsov (UCI: University of California, Irvine)H-Index: 16
#2Alexander McPherson (UCI: University of California, Irvine)H-Index: 53
In the course of an atomic force microscopy investigation of mimivirus, we observed that disrupted virions released masses of fibers that were several hundreds of nanometers in length and that could not be explained as nucleic acid or polysaccharide. The fibers exhibited a strong 7 nm periodicity along their lengths. They existed singly, and also as ribbons, cables, and in multi stranded coils. In the aggregate structures, the periodic bands of the individual fibers aligned laterally to produce ...
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