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Gary Walsh
University of Limerick
99Publications
23H-index
3,612Citations
Publications 99
Newest
#1Jayne Murphy (UL: University of Limerick)
#2Gary Walsh (UL: University of Limerick)H-Index: 23
Intracellular β-galactosidase (E.C 3.2.1.23) produced by the thermoacidophilic archeon Picrophilus torridus DSM 9790 was purified to homogeneity using a combination of DEAE Sepharose, gel filtration, hydroxyapatite and chromatofocusing chromatographies. LC–MS/MS analysis was used to confirm the identity of the purified protein. The enzyme was found to be a homotrimer, with a molecular mass of 157.0 kDa and an isoelectric point of 5.7. To our knowledge, this enzyme has the lowest pH optimum of an...
Monoclonal antibodies (mAbs) continue to reign supreme, although cellular and gene therapies are slowly starting to gather momentum. Burgeoning growth in biosimilars may threaten future brand monopolies for mAbs and other biologics.
#1Angela Boyce (UL: University of Limerick)H-Index: 8
#2Gary Walsh (UL: University of Limerick)H-Index: 23
An endo-1,4-β-d-glucanase gene was cloned from the thermophilic archaea Sulfolobus shibatae and expressed in E. coli. The recombinant enzyme was purified by heat denaturation and affinity chromatography prior to characterisation. The purified recombinant enzyme exhibited maximum activity at 95–100 °C and displayed a broad pH profile with over 91% of its maximum activity observed at pH 3–5. Upon assessment of enzyme thermal stability, full activity was observed after 1 h incubation at 75, 80 and ...
#1Angela Boyce (UL: University of Limerick)H-Index: 8
#2Gary Walsh (UL: University of Limerick)H-Index: 23
A locally isolated strain of Aspergillus niger van Tieghem was found to produce thermostable β-xylosidase activity. The enzyme was purified by cation and anion exchange and hydrophobic interaction chromatography. Maximum activity was observed at 70–75 °C and pH 4.5. The enzyme was found to be thermostable retaining 91 and 87% of its original activity after incubation for 72 h at 60 and 65 °C, respectively, with 52% residual activity detected after 18 h at 70 °C. Available data indicates that the...
#1Michael P. Ryan (UL: University of Limerick)H-Index: 9
#2Gary Walsh (UL: University of Limerick)H-Index: 23
Whey is a highly polluting by-product of cheese and casein powder manufacture with worldwide production of whey estimated at around 190 × 106 ton/year and growing. Historically whey was considered a burdensome, environmentally damaging by-product. In the last decades however, much research has gone into finding viable alternatives for whey rather than just disposing of it. Multiple biotechnological avenues have been explored and in some cases exploited to turn this waste product into a valuable ...
#1Angela Boyce (UL: University of Limerick)H-Index: 8
#2Gary Walsh (UL: University of Limerick)H-Index: 23
A novel endoglucanase encoding gene was cloned from Alicyclobacillus vulcanalis and expressed in E. coli. The deduced amino acid sequence showed highest identity with α-l-arabinofuranosidase-like proteins from glycoside hydrolase family 51. The recombinant enzyme was purified by affinity chromatography and characterised in terms of its potential suitability for lignocellulose hydrolysis at high temperature in the production of bioethanol. The purified enzyme displayed maximum activity at 80 °C a...
#1Gary Walsh (UL: University of Limerick)H-Index: 23
#1Gary Walsh (UL: University of Limerick)H-Index: 23
#1Gary Walsh (UL: University of Limerick)H-Index: 23
#1Gary Walsh (UL: University of Limerick)H-Index: 23
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