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Andrea M. Andrews
Cardiff University
CatalasePhycomyces blakesleeanusFibroblastHydrogen peroxideBiochemistry
5Publications
5H-index
186Citations
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Publications 5
Newest
#1Lip Yong Chung (Cardiff University)H-Index: 19
#2Richard J. Schmidt (Cardiff University)H-Index: 20
Last. Terence D. Turner (Cardiff University)H-Index: 7
view all 6 authors...
Agaricus bisporus, Fusarium graminearum, Phycomyces blakesleeanus, unbleached and bleached, Rhizomucor miehei, and Rhizopus oryzae were examined as sources of fungal chitin/chitosan. The nitrogen content of the alkali-treated mycelia/sporangiophores obtained after optimization of culture conditions, and of similarly treated A. bisporus stipes, was 2.87, 1.29, 6.27, 6.50, 4.80, and 4.95% w/w, respectively, which relates to an estimated chitin content of 42, 19, 91, 94, 70, and 72%, respectively. ...
19 CitationsSource
#1Lip Yong Chung (Cardiff University)H-Index: 19
#2Richard J. Schmidt (Cardiff University)H-Index: 20
Last. Terence D. Turner (Cardiff University)H-Index: 7
view all 6 authors...
Aspergillus oryzae, Mucor mucedo, and Phycomyces blakesleeanus cultures were examined as sources of chitin/chitosan. The nitrogen content of the alkali-treated mycelia/sporangiophores of A. oryzae, M. mucedo, and P. blakesleeanus was 2.52, 3.61, and 6.27% w/w, which relates to an estimated chitin content of 37, 52, and 91%, respectively. The effect of these fungal materials on the rate of proliferation of human F1000 fibroblasts in culture was examined. At 0.01% w/v, all three materials exhibite...
97 CitationsSource
#1Lip Yong Chung (Cardiff University)H-Index: 19
#2Richard J. Schmidt (Cardiff University)H-Index: 20
Last. Terence D. Turner (Cardiff University)H-Index: 7
view all 4 authors...
The effect of Granuflex Hydrocolloid Granules (0.01-0.50% w/v) on the rate of proliferation of murine (L929) fibroblasts was examined. The dose-response curve showed a significant (P<0.02) pro-proliferant effect at 0.05%, and a significant (P<0.02) antiproliferant effect at 0.50%, mirroring the dose-response curve produced by hydrogen peroxide in the concentration range 10 -9 -10 -4 mol/l. The antiproliferant effect at 0.20% w/v was abolished by catalase, suggesting that the biological activity ...
20 CitationsSource
#1Richard J. Schmidt (Cardiff University)H-Index: 20
#2Lip Yong Chung (Cardiff University)H-Index: 19
Last. Terence D. Turner (Cardiff University)H-Index: 7
view all 5 authors...
~~ Abstract-An in-vitro screening method to examine the biocompatibility of materials used in wound management has been evaluated. This involved the use of a macrophage respiratory-burst assay and a fibroblast proliferation assay to represent respectively the inflammatory and the granulation phases in wound healing. Standard polysaccharides (calcium and sodium algnates, t-carrageenan, chitin, chitosan lactate, chondroitin sulphate and pectic acid) were used as test compounds. None of the polysac...
38 CitationsSource
#1Richard J. Schmidt (Cardiff University)H-Index: 20
#2Lip Yong Chung (Cardiff University)H-Index: 19
Last. Terence D. Turner (Cardiff University)H-Index: 7
view all 4 authors...
Fibroblast cultures are often used to evaluate materials intended for medical use, cytotoxicity being taken as an indicator of bioincompatibility. Such an approach has previously been taken with ascorbic acid in determining its value in wound healing. We have now reexamined the toxicity of L-ascorbic acid to L929 fibroblast cells in culture. Concentrations of ascorbic acid between 0.5 mM and 11 mM were tested. At concentrations above 2 mM, ascorbic acid was found to inhibit cell proliferation, w...
12 CitationsSource
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