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Claire R. Shen
National Tsing Hua University
EnzymeEscherichia coliBiochemistryMetabolic engineeringBiology
25Publications
13H-index
2,223Citations
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Publications 25
Newest
#1Eric S. Fan (NTHU: National Tsing Hua University)H-Index: 1
#2Ken W. Lu (NTHU: National Tsing Hua University)H-Index: 1
Last. Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
view all 4 authors...
Photosynthetic generation of reducing power makes cyanobacteria an attractive host for biochemical reduction compared to cell-free and heterotrophic systems, which require burning of additional resources for the supply of reducing equivalent. Here, using xylitol synthesis as an example, we demonstrated efficient uptake and reduction of xylose photoautotrophically in Synechococcus elongatus PCC7942 upon introduction of an effective xylose transporter from Escherichia coli (Ec-XylE) and the NADPH-...
1 CitationsSource
#1Tzu‐Yu Lin (NTUST: National Taiwan University of Science and Technology)H-Index: 1
#2Rex C. Wen (NTHU: National Tsing Hua University)H-Index: 2
Last. Shen-Long Tsai (NTUST: National Taiwan University of Science and Technology)H-Index: 16
view all 4 authors...
2,5-furandicarboxylic acid (FDCA) is one of the top platform chemicals that can be produced from biomass feedstock. To make the cost of industrial FDCA production compatible with plastics made from fossils, the price of substrates and process complexity should be reduced. The aim of this research was to create a CO2 -driven syntrophic consortium for the catalytic conversion of renewable biomass-derived 5-hydroxymethylfurfural (HMF) to FDCA. Sucrose produced from carbon fixation by the engineered...
1 CitationsSource
#1Sastia Prama Putri (Osaka University)H-Index: 11
#2Yasumune Nakayama (Osaka University)H-Index: 11
Last. Eiichiro Fukusaki (Osaka University)H-Index: 48
view all 9 authors...
Introduction Previously constructed Escherichia coli strains that produce 1-propanol use the native threonine pathway, or a heterologous citramalate pathway. However, based on the energy and cofactor requirements of each pathway, a combination of the two pathways produces synergistic effects that increase the theoretical maximum yield with a simultaneous unexplained increase in productivity.
Source
#1Keming Liang (NTHU: National Tsing Hua University)H-Index: 2
#2Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
Enzymatic reduction of acetoin into 2,3-butanediol (2,3-BD) typically requires the reduced nicotinamide adenine dinucleotide (NADH) or its phosphate form (NADPH) as electron donor. Efficiency of 2,3-BD biosynthesis, therefore, is heavily influenced by the enzyme specificity and the cofactor availability which varies dynamically. This work describes the engineering of cofactor flexibility for 2,3-BD production by simultaneous overexpression of an NADH-dependent 2,3-BD dehydrogenase from Klebsiell...
4 CitationsSource
#1Grey S. Chen (NTHU: National Tsing Hua University)H-Index: 2
#2Siang Wun Siao (NTHU: National Tsing Hua University)H-Index: 1
Last. Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
view all 3 authors...
Iterative ketoacid elongation has been an essential tool in engineering artificial metabolism, in particular the synthetic alcohols. However, precise control of product specificity is still greatly challenged by the substrate promiscuity of the ketoacid decarboxylase, which unselectively hijacks ketoacid intermediates from the elongation cycle along with the target ketoacid. In this work, preferential tuning of the Lactococcus lactis ketoisovalerate decarboxylase (Kivd) specificity toward 1-pent...
10 CitationsSource
#1Peiching Chang (NTHU: National Tsing Hua University)H-Index: 1
#2Grey S. Chen (NTHU: National Tsing Hua University)H-Index: 2
Last. Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
view all 5 authors...
Abstract Itaconic acid is an excellent polymeric precursor with wide range of industrial applications. Here, efficient production of itaconate from various renewable substrates was demonstrated by engineered Escherichia coli . Limitation in the itaconate precursor supply was revealed by feeding of the key intermediate citrate to the culture medium. Efforts of enhancing the cis -aconitate flux and preserving the isocitrate pool increased itaconate productivity by nearly 100-fold. Elimination of t...
7 CitationsSource
#1Keming Liang (NTHU: National Tsing Hua University)H-Index: 2
#2Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
Abstract Fermentative redox balance has long been utilized as a metabolic evolution platform to improve efficiency of NADH-dependent pathways. However, such system relies on the complete recycling of NADH and may become limited when the target pathway results in excess NADH stoichiometrically. In this study, endogenous capability of Escherichia coli for 2,3-butanediol (2,3-BD) synthesis was explored using the anaerobic selection platform based on redox balance. To address the issue of NADH exces...
7 CitationsSource
#1Rex C. Wen (NTHU: National Tsing Hua University)H-Index: 2
#2Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
As a natural fermentation product secreted by Clostridium species, bio-based 1-butanol has attracted great attention for its potential as alternative fuel and chemical feedstock. Feasibility of microbial 1-butanol production has also been demonstrated in various recombinant hosts. In this work, we constructed a self-regulated 1-butanol production system in Escherichia coli by borrowing its endogenous fermentation regulatory elements (FRE) to automatically drive the 1-butanol biosynthetic genes i...
10 CitationsSource
#1Chun-Hung Huang (NTHU: National Tsing Hua University)H-Index: 3
#2Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
Last. HuYu-Chen (NTHU: National Tsing Hua University)H-Index: 44
view all 6 authors...
Background Cyanobacterium Synechococcus elongatus PCC 7942 holds promise for biochemical conversion, but gene deletion in PCC 7942 is time-consuming and may be lethal to cells. CRISPR interference (CRISPRi) is an emerging technology that exploits the catalytically inactive Cas9 (dCas9) and single guide RNA (sgRNA) to repress sequence-specific genes without the need of gene knockout, and is repurposed to rewire metabolic networks in various procaryotic cells.
41 CitationsSource
#1Hung Li (NTHU: National Tsing Hua University)H-Index: 3
#2Claire R. Shen (NTHU: National Tsing Hua University)H-Index: 13
Last. HuYu-Chen (NTHU: National Tsing Hua University)H-Index: 44
view all 6 authors...
Abstract Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus PCC 7942 poses challenges because of their oligoploidy nature and long-term instability of the introduced gene. CRISPR-Cas9 is a newly developed RNA-guided genome editing system, yet its application for cyanobacteria engineering has yet to be reported. Here we demonstrated that CRISPR-Cas9 system can effectively trigger pr...
58 CitationsSource
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