Valentin Borshchevskiy
Moscow Institute of Physics and Technology
Publications 51
Published on Sep 12, 2019in bioRxiv
Kirill Kovalev5
Estimated H-index: 5
Dmytro Volkov (Forschungszentrum Jülich)+ 12 AuthorsAlexander Popov18
Estimated H-index: 18
(European Synchrotron Radiation Facility)
Rhodopsins are the most abundant light-harvesting proteins. A new family of rhodopsins, heliorhodopsins (HeRs), was recently discovered. In opposite to the known rhodopsins their N-termini face the cytoplasm. HeRs structure and function remain unknown. We present structures of two HeR-48C12 states at 1.5 A showing its remarkable difference from all known rhodopsins. Its internal extracellular part is completely hydrophobic, while the cytoplasmic part comprises a cavity (‘active site’), surrounde...
Published on Jun 11, 2019in Expert Opinion on Drug Discovery4.42
Alexey Mishin5
Estimated H-index: 5
(MIPT: Moscow Institute of Physics and Technology),
Anastasiia Gusach (MIPT: Moscow Institute of Physics and Technology)+ 3 AuthorsVadim Cherezov60
Estimated H-index: 60
(SC: University of Southern California)
ABSTRACTIntroduction: X-ray crystallography has made important contributions to modern drug development but its application to many important drug targets has been extremely challenging. The recent emergence of X-ray free electron lasers (XFELs) and advancements in serial femtosecond crystallography (SFX) have offered new opportunities to overcome limitations of traditional crystallography to accelerate the structure-based drug discovery (SBDD) process.Areas covered: In this review, the authors ...
Published on Jan 1, 2019in Photochemical and Photobiological Sciences2.41
Vera V. Nazarenko (MIPT: Moscow Institute of Physics and Technology), V.V. Nazarenko (MIPT: Moscow Institute of Physics and Technology)+ 15 AuthorsA. V. Rogachev6
Estimated H-index: 6
Light-Oxygen-Voltage (LOV) domains are conserved parts of photoreceptors in plants, bacteria and fungi that bind flavins as chromophores and detect blue light. In the past, LOV domain variants have been developed as fluorescent reporter proteins (called flavin-based fluorescent proteins; FbFPs), which due to their ability to fluoresce under anaerobic conditions, fast folding kinetics and a small size of ∼12–16 kDa are a promising reporter system for quantitative real-time analysis of biological ...
Published on Apr 1, 2019in Science Advances
Kirill Kovalev5
Estimated H-index: 5
Vitaly Polovinkin7
Estimated H-index: 7
(CNRS: Centre national de la recherche scientifique)
+ 12 AuthorsDmitry Bratanov1
Estimated H-index: 1
(CNRS: Centre national de la recherche scientifique)
Rhodopsins are the most universal biological light-energy transducers and abundant phototrophic mechanisms that evolved on Earth and have a remarkable diversity and potential for biotechnological applications. Recently, the first sodium-pumping rhodopsin KR2 from Krokinobacter eikastus was discovered and characterized. However, the existing structures of KR2 are contradictory, and the mechanism of Na + pumping is not yet understood. Here, we present a structure of the cationic (non H + ) light-d...
Published on Dec 1, 2018in Scientific Reports4.01
Ivan Maslov1
Estimated H-index: 1
(MIPT: Moscow Institute of Physics and Technology),
Andrey O. Bogorodskiy2
Estimated H-index: 2
(MIPT: Moscow Institute of Physics and Technology)
+ 9 AuthorsValentin I. Gordeliy21
Estimated H-index: 21
(MIPT: Moscow Institute of Physics and Technology)
Research on halophilic microorganisms is important due to their relation to fundamental questions of survival of living organisms in a hostile environment. Here we introduce a novel method to stain halophiles with MitoTracker fluorescent dyes in their growth medium. The method is based on membrane-potential sensitive dyes, which were originally used to label mitochondria in eukaryotic cells. We demonstrate that these fluorescent dyes provide high staining efficiency and are beneficial for multi-...