Match!
Seongmin Lee
University of Texas at Austin
Organic chemistryChemistryDNA polymeraseDNABiochemistry
59Publications
16H-index
628Citations
What is this?
Publications 66
Newest
#1Myong-Chul Koag (University of Texas at Austin)H-Index: 3
#2Hunmin Jung (University of Texas at Austin)
Last. Seongmin Lee (University of Texas at Austin)H-Index: 16
view all 3 authors...
Reactive oxygen species generate the genotoxic 8-oxoguanine (oxoG) and 8-oxoadenine (oxoA) as major oxidative lesions. The mutagenicity of oxoG is attributed to the lesion's ability to evade the geometric discrimination of DNA polymerases by adopting Hoogsteen base pairing with adenine in a Watson-Crick-like geometry. Compared with oxoG, the mutagenesis mechanism of oxoA, which preferentially induces A-to-C mutations, is poorly understood. In the absence of protein contacts, oxoA:G forms a wobbl...
Source
#1Hala Ouzon-Shubeita (University of Texas at Austin)H-Index: 3
#2Hunmin Jung (University of Texas at Austin)
Last. Seongmin Lee (University of Texas at Austin)H-Index: 16
view all 5 authors...
Thymine:guanine base pairs are major promutagenic mismatches occurring in DNA metabolism. If left unrepaired, these mispairs can cause C to T transition mutations. In humans, T:G mismatches are repaired in part by mismatch-specific DNA glycosylases such as methyl-CpG-binding domain 4 (hMBD4) and thymine DNA glycosylase. Unlike lesion-specific DNA glycosylases, T:G-mismatch-specific DNA glycosylases specifically recognize both bases of the mismatch and remove the thymine but only from mispairs wi...
Source
#1Hala Ouzon-Shubeita (University of Texas at Austin)H-Index: 3
#2Caroline K. Vilas (University of Texas at Austin)H-Index: 1
Last. Seongmin Lee (University of Texas at Austin)H-Index: 16
view all 3 authors...
The cisplatin-1,2-d(GpG) (Pt-GG) intrastrand cross-link is the predominant DNA lesion generated by cisplatin. Cisplatin has been shown to predominantly induce G to T mutations and Pt-GG permits significant misincorporation of dATP by human DNA polymerase beta (polbeta). In agreement, polbeta overexpression, which is frequently observed in cancer cells, is linked to cisplatin resistance and a mutator phenotype. However, the structural basis for the misincorporation of dATP opposite Pt-GG is unkno...
Source
#1Myong-Chul KoagH-Index: 3
Last. Seongmin LeeH-Index: 16
view all 4 authors...
A wide range of endogenous and exogenous alkylating agents attack DNA to generate various alkylation adducts. N7-methyl-2-deoxyguanosine (Fm7dG) is the most abundant alkylative DNA lesion. If not repaired, Fm7dG can undergo spontaneous depurination, imidazole ring-opening, or bypass by translesion synthesis DNA polymerases. Human DNA polymerase η (polη) efficiently catalyzes across Fm7dG in vitro, but its structural basis is unknown. Herein, we report a crystal structure of polη in complex with ...
Source
#1Yi KouH-Index: 7
#2Myong Chul Koag (University of Texas at Austin)H-Index: 8
Last. Seongmin LeeH-Index: 16
view all 3 authors...
Chronic inflammation is closely associated with cancer development. One possible mechanism for inflammation-induced carcinogenesis is DNA damage caused by reactive halogen species, such as hypochlorous acid, which is released by myeloperoxidase to kill pathogens. Hypochlorous acid can attack genomic DNA to produce 8-chloro-2′-deoxyguanosine (ClG) as a major lesion. It has been postulated that ClG promotes mutagenic replication using its syn conformer; yet, the structural basis for ClG-induced mu...
1 CitationsSource
#1Myong-Chul Koag (University of Texas at Austin)H-Index: 3
#2Hunmin Jung (University of Texas at Austin)
Last. Seongmin Lee (University of Texas at Austin)H-Index: 16
view all 3 authors...
Reactive oxygen species attack DNA to produce 7,8-dihyro-8-oxoguanine (oxoG) and 7,8-dihydro-8-oxoadenine (oxoA) as major lesions. The structural basis for the mutagenicity of oxoG, which induces G to T mutations, is well understood. However, the structural basis for the mutagenic potential of oxoA, which induces A to C mutations, remains poorly understood. To gain insight into oxoA-induced mutagenesis, we conducted kinetic studies of human DNA polymerases β and η replicating across oxoA and str...
Source
#1Aaron Leland Rozelle (University of Texas at Austin)
#2Rayala Naveen Kumar (University of Texas at Austin)H-Index: 5
Last. Seongmin Lee (University of Texas at Austin)H-Index: 16
view all 3 authors...
: Coumarins are a class of naturally occurring compounds that have been shown to form photochemical DNA interstrand cross-links (ICLs). However, study of a coumarin base has not been explored. Using nucleophilic substitution and phosphoramidite chemistry, we synthesized a coumarin base-containing oligonucleotide. Upon exposure to long-wave ultraviolet light, the coumarin-modified oligonucleotide formed ICLs with complementary oligonucleotide containing dT and dC opposite the coumarin base, presu...
Source
#1Hala Ouzon-Shubeita (University of Texas at Austin)H-Index: 3
#2Meghan Baker (University of Texas at Austin)H-Index: 1
Last. Seongmin Lee (University of Texas at Austin)H-Index: 16
view all 4 authors...
Oxaliplatin, together with cisplatin, is among the most important drugs used in cancer chemotherapy. Oxaliplatin, which contains a bulky diaminocyclohexane (DACH) moiety, kills cancer cells mainly by producing (DACH)Pt–GpG intrastrand cross-links that impede transcription. The Pt–GpG tolerance by translesion DNA synthesis (TLS) polymerases contributes to the resistance of tumors to platinum-based chemotherapy. In particular, human DNA polymerase η (Polη) readily bypasses Pt–GpG adducts. While ma...
4 CitationsSource
#1Yi Kou (University of Texas at Austin)H-Index: 7
#2Myong-Chul Koag (University of Texas at Austin)H-Index: 3
Last. Seongmin Lee (University of Texas at Austin)H-Index: 16
view all 3 authors...
A wide variety of endogenous and exogenous alkylating agents attack DNA to preferentially generate N7-alkylguanine (N7-alkylG) adducts. Studies of the effect of N7-alkylG lesions on biological processes have been difficult in part because of complications arising from the chemical lability of the positively charged N7-alkylG, which can readily produce secondary lesions. To assess the effect of bulky N7-alkylG on DNA replication, we prepared chemically stable N7-benzylguanine (N7bnG)-containing D...
8 CitationsSource
#1Myong-Chul Koag (University of Texas at Austin)H-Index: 3
#2Seongmin Lee (University of Texas at Austin)H-Index: 16
DNA polymerases accommodate various base pair conformations in the event of incorrect insertions. In particular, Watson-Crick-like dG:dTTP base pair has been observed at the insertion site of human DNA polymerase β (pol β). A potential factor contributing to the diverse conformations of base pair mismatches is minor groove interactions. To gain insight into the effect of minor groove interactions on base pair conformations, we generated an Asn279Ala polβ mutant that cannot make minor groove cont...
Source
1234567