Doron Gerber
Bar-Ilan University
Publications 59
#1Hadas Nevenzal (BIU: Bar-Ilan University)H-Index: 2
#2Meirav Noach-Hirsh (BIU: Bar-Ilan University)H-Index: 2
Last.Doron Gerber (BIU: Bar-Ilan University)H-Index: 21
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Autophosphorylation of receptor and non-receptor tyrosine kinases is a common molecular switch with broad implications for pathogeneses and therapy of cancer and other human diseases. Technologies for large-scale discovery and analysis of autophosphorylation are limited by the inherent difficulty to distinguish between phosphorylation and autophosphorylation in vivo and by the complexity associated with functional assays of receptors kinases in vitro. Here, we report a method for the direct dete...
#1K. Shanmugha Rajan (BIU: Bar-Ilan University)H-Index: 3
#2Tirza Doniger (BIU: Bar-Ilan University)H-Index: 11
Last.Shulamit Michaeli (BIU: Bar-Ilan University)H-Index: 31
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2 CitationsSource
#1Orna Ernst (TAU: Tel Aviv University)H-Index: 6
#2Yifat Glucksam-Galnoy (TAU: Tel Aviv University)H-Index: 4
Last.Tsaffrir Zor (TAU: Tel Aviv University)H-Index: 19
view all 8 authors...
Expression of the key anti-inflammatory cytokine IL-10 in lipopolysaccharide (LPS)-stimulated macrophages is mediated by a delayed autocrine / paracrine loop of type I interferons (IFN) to ensure timely attenuation of inflammation. We have previously shown that cAMP synergizes with early IL-10 expression by LPS, but is unable to amplify the late type I IFN-dependent activity. We now examined the mechanism of this synergistic transcription in mouse macrophages at the promoter level, and explored ...
Last.Amit TzurH-Index: 8
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E2F8 is a transcriptional repressor that antagonizes the canonical cell cycle transcription factor E2F1. Despite the importance of this atypical E2F family member in cell cycle, apoptosis and cancer, we lack a complete description of the mechanisms that control its dynamics. To address this question, we developed a complementary set of static and dynamic cell-free systems of human origin, which recapitulate inter-mitotic and G1 phases, and a full transition from pro-metaphase to G1. This reveale...
Last.Doron GerberH-Index: 21
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#1Mirit HenH-Index: 1
#2Eitan EdriH-Index: 2
Last.Chaim N. SukenikH-Index: 29
view all 7 authors...
Interest in polydimethylsiloxane (PDMS) microfluidic devices has grown dramatically in recent years, particularly in the context of improved performance lab-on-a-chip devices with decreasing channel size enabling more devices on ever smaller chips. As channels become smaller, the resistance to flow increases and the device structure must be able to withstand higher internal pressures. We report herein the fabrication of microstructured surfaces that promote water mobility independent of surface ...
#1Nika Schwartz (BIU: Bar-Ilan University)H-Index: 1
#2Michal Pellach (BIU: Bar-Ilan University)H-Index: 6
Last.Doron Gerber (BIU: Bar-Ilan University)H-Index: 21
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Abstract The hepatitis C virus (HCV) non-structural protein 3 (NS3) is essential for HCV maturation. The NS3/4A protease is a target for several HCV treatments and is a well-known target for HCV drug discovery. The protein is membrane associated and thus probably interacts with other membrane proteins. However, the vast majority of known NS3 host partners are soluble proteins rather than membrane proteins, most likely due to lack of appropriate platforms for their discovery. Utilization of an in...
1 CitationsSource
#1Maria Ronen (BIU: Bar-Ilan University)H-Index: 3
#2Basanth S. Kalanoor (BIU: Bar-Ilan University)H-Index: 4
Last.Doron Gerber (BIU: Bar-Ilan University)H-Index: 21
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Low Frequency Vibrational (LFV) modes of peptides and proteins are attributed to the lattice vibrations and are dependent on their structural organization and self-assembly. Studies taken in order to assign specific absorption bands in the low frequency range to self-assembly behavior of peptides and proteins have been challenging. Here we used a single stage Low Frequency Raman (LF-Raman) spectrometer to study a series of diastereomeric analogue peptides to investigate the effect of peptides se...
1 CitationsSource
#1Toma E. TomovH-Index: 10
#2Roman TsukanovH-Index: 12
Last.Eyal NirH-Index: 26
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Realization of bioinspired molecular machines that can perform many and diverse operations in response to external chemical commands is a major goal in nanotechnology, but current molecular machines respond to only a few sequential commands. Lack of effective methods for introduction and removal of command compounds and low efficiencies of the reactions involved are major reasons for the limited performance. We introduce here a user interface based on a microfluidics device and single-molecule f...
21 CitationsSource
#2Maria RonenH-Index: 3
Last.Yaakov R. TischlerH-Index: 9
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The low-frequency vibrational (LFV) modes of biomolecules reflect specific intramolecular and intermolecular thermally induced fluctuations that are driven by external perturbations, such as ligand binding, protein interaction, electron transfer, and enzymatic activity. Large efforts have been invested over the years to develop methods to access the LFV modes due to their importance in the studies of the mechanisms and biological functions of biomolecules. Here, we present a method to measure th...
7 CitationsSource