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Tanay M. Desai
Emory University
EndosomeVirologyVirusViral entryBiology
14Publications
8H-index
257Citations
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Publications 14
Newest
#1Krishna C. Suddala (Emory University)H-Index: 8
#2Christine C. Lee (Emory University)H-Index: 1
Last. Gregory B. Melikyan (Emory University)H-Index: 23
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Late endosome-resident interferon-induced transmembrane protein 3 (IFITM3) inhibits fusion of diverse viruses, including Influenza A virus (IAV), by a poorly understood mechanism. Despite the broad antiviral activity of IFITM3, viruses like Lassa virus (LASV), are fully resistant to its inhibitory effects. It is currently unclear whether resistance arises from a highly efficient fusion machinery that is capable of overcoming IFITM3 restriction or the ability to enter from cellular sites devoid o...
3 CitationsSource
#1Chetan SoodH-Index: 4
Last. Gregory B. MelikyanH-Index: 23
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#1Chetan Sood (Emory University)H-Index: 4
#2Ashwanth C. Francis (Emory University)H-Index: 5
Last. Gregory B. Melikyan (Emory University)H-Index: 23
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Abstract Enveloped viruses transfer their genomes into host cells by fusing their membrane to that of the cell. To visualize single-virus fusion in living cells, researchers take advantage of HIV-1's proteolytic maturation, which can generate free fluorescent proteins within the viral particle. Co-labeling viruses with a content marker and a fluorescently tagged Vpr (a viral core protein) enables detection of single-virus fusions, but a major limitation of this approach is that not all viral par...
7 CitationsSource
#1Tanay M. Desai (Emory University)H-Index: 8
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
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Abstract Enveloped viruses infect host cells by fusing their membranes with those of the host cell, a process mediated by viral glycoproteins upon binding to cognate host receptors or entering into acidic intracellular compartments. Whereas the effect of receptor density on viral infection has been well studied, the role of cell type-specific factors/processes, such as pH regulation, has not been characterized in sufficient detail. Here, we examined the effects of cell-extrinsic factors (buffer ...
8 CitationsSource
#1Cheri M. HamptonH-Index: 9
#2Joshua D. StraussH-Index: 6
Last. Elizabeth R. WrightH-Index: 25
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Correlative light and electron microscopy (CLEM) promises new insight into cellular processes by combining spatiotemporal information with high-resolution structural information. This protocol describes cryo-CLEM of virus-infected mammalian cells.
40 CitationsSource
#1Yoonhyeun Oum (Emory University)H-Index: 5
#2Tanay M. Desai (Emory University)H-Index: 8
Last. Gregory B. Melikyan (Emory University)H-Index: 23
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Enveloped viruses infect target cells by fusing their membrane with cellular membrane through a process that is mediated by specialized viral glycoproteins. The inefficient and highly asynchronous nature of viral fusion complicates studies of virus entry on a population level. Single virus imaging in living cells has become an important tool for delineating the entry pathways and for mechanistic studies of viral fusion. We have previously demonstrated that incorporation of fluorescent labels int...
8 CitationsSource
Source
#1Tanay M. Desai (Emory University)H-Index: 8
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
view all 7 authors...
Background HIV-1 Vpr is recruited into virions during assembly and appears to remain associated with the viral core after the reverse transcription and uncoating steps of entry. This feature has prompted the use of fluorescently labeled Vpr to visualize viral particles and to follow trafficking of post-fusion HIV-1 cores in the cytoplasm.
18 CitationsSource
#1Naoyuki Kondo (Emory University)H-Index: 12
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
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Whether HIV-1 enters cells by fusing with the plasma membrane or with endosomes is a subject of active debate. The ability of HIV-1 to mediate fusion between adjacent cells, a process referred to as “fusion-from-without” (FFWO), shows that this virus can fuse with the plasma membrane. To compare FFWO occurring at the cell surface with HIV-cell fusion through a conventional entry route, we designed an experimental approach that enabled the measurements of both processes in the same sample. The fo...
17 CitationsSource
#1Tanay M. Desai (Emory University)H-Index: 8
#2Mariana Marin (Emory University)H-Index: 18
Last. Gregory B. Melikyan (Emory University)H-Index: 23
view all 3 authors...
Viral protein R (Vpr) is an HIV-1 accessory protein that associates with capsids during viral assembly and is important for infections in non-dividing cells. Vpr functions in host cells include induction of G2 cell-cycle arrest, and regulation of cellular proliferation and apoptosis. Vpr has two nuclear localization sequences that direct its transport to the nucleus. Fluorescently labeled Vpr (YFP-Vpr) is widely used to visualize HIV-1 cores in the cytoplasm during entry. Here we report on the d...
1 CitationsSource
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