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Stephanie A. Smith
University of Illinois at Urbana–Champaign
ThrombinPolyphosphateBiochemistryMedicinePlatelet
97Publications
31H-index
3,846Citations
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#1Catherine J. Baker (UM: University of Michigan)H-Index: 4
#2Stephanie A. Smith (UM: University of Michigan)H-Index: 31
Last. James H. Morrissey (UM: University of Michigan)H-Index: 52
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: This illustrated review focuses on polyphosphate as a potent modulator of the plasma clotting cascade, with possible roles in hemostasis, thrombosis, and inflammation. Polyphosphates are highly anionic, linear polymers of inorganic phosphates that are widespread throughout biology. Infectious microorganisms accumulate polyphosphates with widely varying polymer lengths (from a few phosphates to over a thousand phosphates long), while activated human platelets secrete polyphosphate with a very n...
4 CitationsSource
#1Raquel S. Negreiros (UGA: University of Georgia)H-Index: 1
#2Noelia Lander (UGA: University of Georgia)H-Index: 13
Last. Roberto Docampo (UGA: University of Georgia)H-Index: 65
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: Inorganic polyphosphate (polyP) is a polymer of three to hundreds of phosphate units bound by high-energy phosphoanhydride bonds and present from bacteria to humans. Most polyP in trypanosomatids is concentrated in acidocalcisomes, acidic calcium stores that possess a number of pumps, exchangers, and channels, and are important for their survival. In this work, using polyP as bait we identified > 25 putative protein targets in cell lysates of both Trypanosoma cruzi and Trypanosoma brucei. Gene...
8 CitationsSource
#1Stephanie A. Smith (UM: University of Michigan)H-Index: 31
#2Yan Wang (Bayer)H-Index: 1
Last. James H. Morrissey (UM: University of Michigan)H-Index: 52
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: PAGE is often used to resolve inorganic polyphosphates (polyP), but unfortunately polyP size ladders are not commercially available. Since several dyes that are commonly used to detect nucleic acids in gels also stain polyP, we examined the utility of commercially available DNA size ladders for estimating polyP polymer lengths by gel electrophoresis. Narrow size fractions of polyP were prepared and their polymer lengths were quantified using NMR. Commercially available DNA ladders and these po...
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Objective— Terminal complications of bacterial sepsis include development of disseminated intravascular consumptive coagulopathy. Bacterial constituents, including long-chain polyphosphates (polyP)...
4 CitationsSource
#1Jevgenia Zilberman-Rudenko (OHSU: Oregon Health & Science University)H-Index: 7
Last. Owen J. T. McCarty (OHSU: Oregon Health & Science University)H-Index: 38
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#1Jevgenia Zilberman-Rudenko (OHSU: Oregon Health & Science University)H-Index: 7
Last. Owen J. T. McCarty (OHSU: Oregon Health & Science University)H-Index: 38
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#1Stephanie A. Smith (UM: University of Michigan)H-Index: 31
Last. James H. Morrissey (UM: University of Michigan)H-Index: 52
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Polyphosphate plays several roles in coagulation and inflammation, while extracellular DNA and RNA are implicated in thrombosis and as disease biomarkers. We sought to compare the procoagulant activities of polyphosphate versus DNA or RNA isolated from mammalian cells. In a recent study, we found that much of the procoagulant activity of DNA isolated from mammalian cells using Qiagen kits resisted digestion with nuclease or polyphosphatase, and even resisted boiling in acid. These kits employ sp...
6 CitationsSource
#1Corinne Lawson (UIUC: University of Illinois at Urbana–Champaign)H-Index: 2
#2Stephanie A. Smith (UIUC: University of Illinois at Urbana–Champaign)H-Index: 31
Last. Maureen McMichael (UIUC: University of Illinois at Urbana–Champaign)H-Index: 21
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BACKGROUND: Neutrophil extracellular traps (NETs) are part of the innate immune response and are essential in local pathogen control, but are associated with pathological inflammation, organ damage, autoimmunity, and thrombosis. Immune-mediated hemolytic anemia (IMHA) is a pro-inflammatory, prothrombotic disease associated with high mortality. HYPOTHESIS/OBJECTIVES: Neutrophil extracellular traps (NETs) are a feature of the inflammatory process in dogs with IMHA. The objective of the study was t...
6 CitationsSource
#1Stephanie A. Smith (UIUC: University of Illinois at Urbana–Champaign)H-Index: 31
#2Catherine J. Baker (UIUC: University of Illinois at Urbana–Champaign)H-Index: 4
Last. James H. Morrissey (UIUC: University of Illinois at Urbana–Champaign)H-Index: 52
view all 4 authors...
To the editor: Recent advances in understanding the contributions of the contact pathway to thrombosis, inflammation, and innate immunity[1][1] have renewed interest in identifying (patho)physiologic activators of this pathway. Candidates include collagen,[2][2] misfolded proteins,[3][3] DNA,[4][4
11 CitationsSource
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