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Seongkyu Yoon
University of Massachusetts Lowell
Chinese hamster ovary cellChemistryChromatographyBiochemistryCell culture
72Publications
12H-index
878Citations
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Publications 76
Newest
#1Zhuangrong Huang (University of Massachusetts Lowell)H-Index: 5
#2Jianlin Xu (BMS: Bristol-Myers Squibb)H-Index: 11
Last. Seongkyu Yoon (University of Massachusetts Lowell)H-Index: 12
view all 9 authors...
Abstract Effective bioprocess development using Chinese hamster ovary (CHO) cells as hosts is hampered by the limited understanding of cellular metabolism under process conditions in bioreactors. Systematic tools such as genome-scale models have been developed, but their value has not been satisfactorily demonstrated and exploited for process development. In this study, we proposed a method using a genome-scale model to analyze existing process studies for bioprocess optimization. First, we used...
Source
#1Sha Sha (University of Massachusetts Lowell)H-Index: 3
#2Garry J. Handelman (University of Massachusetts Lowell)H-Index: 45
Last. Seongkyu Yoon (University of Massachusetts Lowell)H-Index: 12
view all 4 authors...
N-Linked glycosylation is a cellular process transferring sugars from glycosyl donors to proteins or lipids. Biopharmaceutical products widely produced by culturing mammalian cells such as Chinese hamster ovary (CHO) cells are typically glycosylated during biosynthesis. For some biologics, the N-linked glycan is a critical quality attribute of the drugs. Nucleotide sugars are the glycan donors and impact the intracellular glycosylation process. In current analytical methods, robust separation of...
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#1Ashli L. Polanco (University of Massachusetts Lowell)
#2Bingyu Kuang (University of Massachusetts Lowell)
Last. Seongkyu Yoon (University of Massachusetts Lowell)H-Index: 12
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Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing stem cell bioprocessing strategies that are scalable and meet clinical needs requires incorporating methods that measure and monitor intrinsic markers of cell differentiation state, developmental status...
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#1Ryan J. Graham (University of Massachusetts Lowell)H-Index: 1
#2Stephanie A. Ketcham (CDER: Center for Drug Evaluation and Research)H-Index: 1
Last. Chikkathur N. Madhavarao (CDER: Center for Drug Evaluation and Research)H-Index: 1
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The variability of trace metals in cell culture media is a potential manufacturing concern because it may significantly affect the production and quality of therapeutic proteins. Variability in trace metals in CHO cell culture has been shown to impact critical production metrics such as cell growth, viability, nutrient consumption, and production of recombinant proteins. To better understand the influence of excess supplementation, zinc and copper were initially supplemented with 50-μM concentra...
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#1Shaun C. Galbraith (University of Massachusetts Lowell)H-Index: 3
#2Seo-Young Park (University of Massachusetts Lowell)H-Index: 3
Last. Seongkyu Yoon (University of Massachusetts Lowell)H-Index: 12
view all 9 authors...
Abstract Continuous manufacturing of oral-dosage pharmaceuticals is widely understood to ensure consistent product quality and higher productivity at lower costs. Here, a hybrid flowsheet model is developed in order to link the process variables to the residence time distribution (RTD) of mixed pharmaceutical powders in a continuous direct compression (CDC) process containing two separate powder blending units. An empirical equation is regressed from collected RTD data for one formulation that c...
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#1Ryan J. Graham (University of Massachusetts Lowell)H-Index: 1
#1Ryan J. Graham (University of Massachusetts Lowell)
Last. Seongkyu Yoon (University of Massachusetts Lowell)H-Index: 12
view all 3 authors...
1 CitationsSource
#1Hae‐Woo LeeH-Index: 2
#2Hemlata BhatiaH-Index: 3
Last. Seongkyu YoonH-Index: 12
view all 10 authors...
Source
#1Alessandro MoraH-Index: 1
#2Bernard NabiswaH-Index: 1
Last. Seongkyu Yoon (University of Massachusetts Lowell)H-Index: 12
view all 6 authors...
In Chinese Hamster Ovary (CHO) cell lines, the establishment of the ideal fed-batch regimen promotes metabolic conditions advantageous for the bioproduction of therapeutic molecules. A tailored, cell line-specific feeding scheme is typically defined during process development (PD) activities, through the incorporation of Design of Experiment (DOE) and late stage cell culture approaches. The feeding during early stage cell line development (CLD) was a simplified “one-fits-all” design, inherited f...
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#1Shaun C. Galbraith (University of Massachusetts Lowell)H-Index: 3
#2Bumjoon Cha (University of Massachusetts Lowell)H-Index: 2
Last. Seongkyu Yoon (University of Massachusetts Lowell)H-Index: 12
view all 10 authors...
Abstract A flowsheet model for a continuous direct compression tableting process is developed and applied to process data generated at production scale. The model contains powder feeding, blending and tablet compaction unit operations. The dataset was provided by Merck & Co., Inc., Kenilworth, NJ USA using a GEA ConsiGma™ continuous direct compression process designed for approximately 50 kg/h throughput using a six ingredient, potentially commercial, formulation. A tanks-in-series methodology i...
1 CitationsSource
#1David N. Powers (CDER: Center for Drug Evaluation and Research)H-Index: 2
#2Yifan Wang (CDER: Center for Drug Evaluation and Research)H-Index: 9
Last. Cyrus Agarabi (CDER: Center for Drug Evaluation and Research)H-Index: 11
view all 9 authors...
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