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Christian Wenz
Agilent Technologies
Molecular biologyChemistryCapillary electrophoresisChromatographyImmunoprecipitation
22Publications
8H-index
369Citations
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Publications 19
Newest
#1Christian WenzH-Index: 8
#2Andreas RüferH-Index: 5
A new method for the targeted analysis of proteins is presented, which combines the specificity of immunoprecipitation with the sensitivity of protein detection on microchips using the High Sensitivity Protein 250 assay for the Agilent 2100 Bioanalyzer. As as an alternative to Western blotting, this method is valuable for researchers engaged, for example, in protein expression and purification in pharma, biotech, or academic labs. Advantages of this new method in comparison to Western blotting a...
#1Nicole Y. Engel (TU Wien: Vienna University of Technology)H-Index: 1
#2Victor U. Weiss (TU Wien: Vienna University of Technology)H-Index: 9
Last. Günter Allmaier (TU Wien: Vienna University of Technology)H-Index: 34
view all 8 authors...
Due to the constant search for reliable methods to investigate glycoproteins in complex biological samples, an alternative approach combining affinity enrichment with rapid and sensitive analysis on-a-chip is presented. Glycoproteins were specifically captured by lectin-coated magnetic beads, eluted by competitive sugars, and investigated with microchip capillary gel electrophoresis (MCGE), i.e., CGE-on-a-chip. We compared our results to sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
1 CitationsSource
#1Christian Wenz (Agilent Technologies)H-Index: 8
#2Coral BarbasH-Index: 45
Last. SungAe Suhr Park (Amgen)H-Index: 5
view all 24 authors...
A collaborative study on the robustness and portability of a capillary electrophoresis-mass spectrometry method for peptide mapping was performed by an international team, consisting of 13 independent laboratories from academia and industry. All participants used the same batch of samples, reagents and coated capillaries to run their assays, whereas they utilized the capillary electrophoresis-mass spectrometry equipment available in their laboratories. The equipment used varied in model, type an...
20 CitationsSource
#1Nicole Y. Engel (TU Wien: Vienna University of Technology)H-Index: 1
#2Victor U. Weiss (TU Wien: Vienna University of Technology)H-Index: 9
Last. Günter Allmaier (TU Wien: Vienna University of Technology)H-Index: 34
view all 8 authors...
Glycosylations severely influence a protein's biological and physicochemical properties. Five exemplary proteins with varying glycan moieties were chosen to establish molecular weight (MW) determination (sizing), quantitation, and sensitivity of detection for microchip capillary gel electrophoresis (MCGE). Although sizing showed increasing deviations from literature values (SDS-PAGE or MALDI-MS) with a concomitant higher degree of analyte glycosylation, the reproducibility of MW determination an...
9 CitationsSource
#1Ela Herwig (TU Wien: Vienna University of Technology)H-Index: 5
#2Martina Marchetti-Deschmann (TU Wien: Vienna University of Technology)H-Index: 15
Last. Günter Allmaier (TU Wien: Vienna University of Technology)H-Index: 34
view all 7 authors...
Abstract Sepsis represents a significant cause of mortality in intensive care units. Early diagnosis of sepsis is essential to increase the survival rate of patients. Among others, C-reactive protein (CRP) is commonly used as a sepsis marker. In this work we introduce immune precipitation combined with microchip capillary gel electrophoresis (IP–MCGE) for the detection and quantification of CRP in serum samples. First high-abundance proteins (HSA, IgG) are removed from serum samples using affini...
6 CitationsSource
Separation and analysis of charge heterogeneity in recombinant protein or monoclonal antibody (mAb) production is a prime quality control step in the biopharmaceutical industry. This step is often carried out by a combination of separation techniques followed by mass spectrometric detection. Two dimensional gel electrophoresis (2D-GE) is unrivalled in terms of resolution but is a tedious procedure. Here we present a combination of two easy methods that separate proteins in analogy to 2D-GE accor...
#1Ela Herwig (TU Wien: Vienna University of Technology)H-Index: 5
#2Martina Marchetti-Deschmann (TU Wien: Vienna University of Technology)H-Index: 15
Last. Günter Allmaier (TU Wien: Vienna University of Technology)H-Index: 34
view all 5 authors...
A sensitive and selective analytical method for the determination and quantification of endogenous β-galactosidase in crude E. coli cell lysates by immunoprecipitation combined with automated microchip capillary gel electrophoresis (IP-MCGE) with laser-induced fluorescence (LIF) detection was developed. Total cell lysates were derivatized minimally with a fluorescence dye, incubated with anti-β-galactosidase antibodies, and the antigen/antibody complex was precipitated with protein G-coated magn...
8 CitationsSource
A new method for the targeted analysis of proteins is presented, which combines the specificity of immunopre cipitation with the sensitivity of protein detection on microchips using the High Sensitivity Protein 250 assay for the Agilent 2100 Bioanalyzer. As as an alternative to Western blotting, this method is valuable for researchers engaged, for example, in protein expression and purification in pharma, biotech, or academic labs. Advantages of this new method in comparison to Western blotting ...
3 CitationsSource
#1Christian Wenz (Agilent Technologies)H-Index: 8
#2R. Salowsky (Agilent Technologies)
Last. A. Ruefer (Agilent Technologies)
view all 6 authors...
Source
#1Christian Wenz (Agilent Technologies)H-Index: 8
#2Martina Marchetti-Deschmann (TU Wien: Vienna University of Technology)H-Index: 15
Last. Andreas Rüfer (Agilent Technologies)H-Index: 5
view all 8 authors...
A novel pre-chip fluorescent derivatization method is presented for protein sizing and quantification by microchip CGE. The derivatization reaction employed a water-soluble and stable fluorescent dye and was performed under conditions that favored the formation of homogeneous reaction products. The method delivered in terms of protein sizing similar results as microchip CGE with on-chip staining but showed an extended linear dynamic range for protein quantification encompassing four orders of ma...
10 CitationsSource
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