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Graham Johnson
Commonwealth Scientific and Industrial Research Organisation
VitronectinOrganic chemistryMaterials sciencePolymerFibronectin
79Publications
24H-index
2,213Citations
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Publications 58
Newest
#2Ronald C. ChatelierH-Index: 24
Last. John G. SteeleH-Index: 25
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Source
#1Donna J. Menzies (Monash University)H-Index: 9
#2Marek Jasieniak (UniSA: University of South Australia)H-Index: 13
Last. Benjamin W. Muir (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 25
view all 7 authors...
Abstract In this work we report a detailed X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) study of poly(ethylene glycol) PEG-like chemical gradients deposited via plasma enhanced chemical vapour deposition (PECVD) at two different load powers using diethylene glycol dimethyl ether (DG) as a monomer. Principal component analysis (PCA) was applied to the ToF-SIMS data both before and after protein adsorption on the plasma polymer thin films. Re...
14 CitationsSource
#1Xiaojuan Hao (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 21
#2Justine Leigh Jeffery (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 8
Last. Timothy C. Hughes (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 20
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Abstract Functionalised siloxane macromonomers, with properties designed for application as an injectable, in situ curable accommodating intraocular lens (A-IOL), were prepared via re-equilibration of a phenyl group-containing polysiloxane of very high molecular weight with octamethylcyclotetrasiloxane (D 4 ) and 2,4,6,8-tetra(n-propyl-3-methacrylate)-2,4,6,8-tetramethyl-cyclotetrasiloxane (D 4 AM ) in toluene using trifluoromethanesulfonic acid as a catalyst. Hexaethyldisiloxane was used as an ...
31 CitationsSource
#1Thomas Ameringer (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 2
#2Peter Fransen (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 1
Last. Laurence Meagher (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 27
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Control over cell-material surface interactions is the key to many new and improved biomedical devices. It can only be achieved if interactions that are mediated by nonspecifically adsorbed serum proteins are minimized and if cells instead respond to specific ligand molecules presented on the surface. Here, we present a simple yet effective surface modification method that allows for the covalent coupling and presentation of specific biological signals on coatings which have significantly reduce...
24 CitationsSource
#1Donna J. Menzies (Monash University)H-Index: 9
#2Thomas R. Gengenbach (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 39
Last. Benjamin W. Muir (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 25
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Micropatterning of surfaces with varying chemical, physical and topographical properties usually requires a number of fabrication steps. Herein, we describe a micropatterning technique based on plasma enhanced chemical vapour deposition (PECVD) that deposits both protein resistant and protein repellent surface chemistries in a single step. The resulting multifunctional, selective surface chemistries are capable of spatially controlled protein adhesion, geometric confinement of cells and the site...
17 CitationsSource
#1Peter Koegler (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 1
#2Paul Pasic (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 6
Last. Helmut Thissen (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 31
view all 7 authors...
Polymeric coatings which allow the effective control of biointerfacial interactions and cellular responses are of increasing interest in a range of biomedical applications in vitro and in vivo such as cell culture tools, biosensors and implantable medical devices. A variety of coating strategies have been developed to gain control over cell-surface interactions but many of them are limited with respect to their function and transferability between different substrate materials. Here, our aim was...
2 CitationsSource
#1Margaret D. M. Evans (UNSW: University of New South Wales)H-Index: 20
#2Hassan Chaouk (UNSW: University of New South Wales)H-Index: 6
Last. Keith M. McLean (UNSW: University of New South Wales)H-Index: 27
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Abstract Design principles for corneal implants are challenging and include permeability which inherently involves pore openings on the polymer surface. These topographical cues can be significant to a successful clinical outcome where a stratified epithelium is needed over the device surface, such as with a corneal onlay or corneal repair material. The impact of polymer surface topography on the growth and adhesion of corneal epithelial tissue was assessed using porous perfluoropolyether membra...
8 CitationsSource
#1Margaret D. M. Evans (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 20
#2Ruby Kala Prakasam (CRCs: Cooperative Research Centre)H-Index: 2
Last. Deborah F. Sweeney (CRCs: Cooperative Research Centre)H-Index: 36
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Abstract This study assessed the long-term biological response of a perfluoropolyether-based polymer developed as a corneal inlay to correct refractive error. The polymer formulation met chemical and physical specifications and was non-cytotoxic when tested using standard in vitro techniques. It was cast into small microporous membranes that were implanted as inlays into corneas of rabbits ( n = 5) and unsighted humans ( n = 5 + 1 surgical control) which were monitored for up to 23 and 48 months...
13 CitationsSource
#1Christopher M. Elvin (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 19
#2Tony Vuocolo (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 28
Last. Jerome A. Werkmeister (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 31
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Gelatin is widely used as a medical biomaterial because it is readily available, cheap, biodegradable and demonstrates favourable biocompatibility. Many applications require stabilisation of the biomaterial by chemical crosslinking, and this often involves derivatisation of the protein or treatment with cytotoxic crosslinking agents. We have previously shown that a facile photochemical method, using blue light, a ruthenium catalyst and a persulphate oxidant, produces covalent di-tyrosine crossli...
85 CitationsSource
#1Lloyd D. Graham (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 19
#2Stephen J. Danon (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 17
Last. John A. M. Ramshaw (CSIRO: Commonwealth Scientific and Industrial Research Organisation)H-Index: 36
view all 11 authors...
When provoked, Notaden bennetti frogs secrete a proteinaceous exudate, which rapidly forms a tacky and elastic glue. This material has potential in biomedical applications. Cultured cells attached and proliferated well on glue-coated tissue culture polystyrene, but migrated somewhat slower than on uncoated surfaces. In organ culture, dissolved glue successfully adhered collagen-coated perfluoropolyether lenses to debrided bovine corneas and supported epithelial regrowth. Small pellets of glue im...
15 CitationsSource
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