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Aipo Diao
Tianjin University of Science and Technology
Molecular biologyChemistryBiochemistryBiologyCell biology
42Publications
10H-index
729Citations
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Publications 42
Newest
#1Lei Jing (Tianjin University of Science and Technology)H-Index: 5
#2Juanjuan Liu (Tianjin University of Science and Technology)
Last. Aipo Diao (Tianjin University of Science and Technology)H-Index: 10
view all 8 authors...
Abstract An affibody is a 58 amino acids peptide derived from the Z domain of staphylococcal protein A and generally applied in areas such as imaging diagnosis, clinical therapeutics and biotechnology research. To screen for an affibody targeting the immune checkpoint PD-L1, a combinatorial affibody library was generated in yeast using degenerate overlap PCR primers and In-fusion technology. Z-j1 and Z-j2 affibodies targeting the Ig-like V domain of PD-L1 were screened and identified from this c...
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#1Zhenxing Liu (Tianjin University of Science and Technology)H-Index: 6
#2Zhichen Shi (Tianjin University of Science and Technology)H-Index: 2
Last. Aipo Diao (Tianjin University of Science and Technology)H-Index: 10
view all 10 authors...
Abstract The transcription factor forkhead box O 3A (FOXO3A) is a tumor suppressor that promotes cell cycle arrest and apoptosis. Piperlongumine (PL), a plant alkaloid, is known to selectively kill tumor cells while sparing normal cells. However, the mechanism of PL-induced cancer cell death is not fully understood. We report here that an association of FOXO3A with the pro-apoptotic protein BIM (also known as BCL2-like 11, BCL2L11) has a direct and specific function in PL-induced cancer cell dea...
4 CitationsSource
#1Wei Li (Inner Mongolia Medical University)H-Index: 2
#2Jieru Lin (Tianjin University of Science and Technology)H-Index: 2
Last. Aipo Diao (Tianjin University of Science and Technology)H-Index: 10
view all 7 authors...
Abstract The autophagy-lysosome pathway plays a central role in cellular homeostasis by regulating the cellular degradative machinery. The transcription factor EB (TFEB) regulates the biogenesis and function of both lysosomes and autophagosomes, and enhancement of TFEB function has emerged as an attractive therapeutic strategy for lysosome-related disorders. However, little is known about the role of TFEB activation in regulating the cellular fate. Here, we describe that clomiphene citrate (CC),...
2 CitationsSource
#1Long Ma (Tianjin University of Science and Technology)H-Index: 18
#2Xiuqi Cao (Tianjin University of Science and Technology)H-Index: 4
Last. Aipo Diao (Tianjin University of Science and Technology)H-Index: 10
view all 12 authors...
Human flap endonuclease 1 (hFEN1) is instrumental in DNA replication and repair. It is able to cleave the 5′ single-stranded protrusion (also known as 5′ flap) resulting from strand displacement reactions. In light of its crucial functions, hFEN1 is now deemed as a nontrivial target in the DNA damage response system for anticancer drug development. Herein, we report that myricetin and some natural flavonoids are able to inhibit hFEN1. Structure–activity relationship, inhibitory mechanisms, molec...
6 CitationsSource
#1Long Ma (Tianjin University of Science and Technology)H-Index: 18
#2Nana Sun (Tianjin University of Science and Technology)H-Index: 5
Last. Aipo Diao (Tianjin University of Science and Technology)H-Index: 10
view all 8 authors...
Abstract In current study, we have found that several magnetic nanoparticles (MNPs) are able to absorb DNA molecules, and surface engineering would be beneficial to tune such interaction. We then have focused on the assembly of polyethylenimine (PEI) coated MNPs (PEI-MNPs) with ssDNA (single-stranded DNA) and found this assembly is mediated by two forces, namely the electrostatic interactions of surface charges of MNPs and the phosphate backbones of DNA; as well as the coordination of exterior i...
9 CitationsSource
#1Yuyin Li (Tianjin University of Science and Technology)H-Index: 8
#2Jianjun Wang (Tianjin University of Science and Technology)H-Index: 2
Last. Aipo Diao (Tianjin University of Science and Technology)H-Index: 10
view all 10 authors...
OBJECTIVES: The transmembrane prostate androgen-induced protein (TMEPAI) is aberrantly expressed in many cancer and plays a crucial role in tumourigenesis, which makes it a potential cancer therapeutic target for drug discovery. MATERIALS AND METHODS: Here, we employed a firefly luciferase reporter driven by the TMEPAI gene promoter to screen for compound capable of inhibiting the expression of TMEPAI, and the effects of TMEPAI inhibitor on cancer cell proliferation were evaluated using the colo...
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#1Tian Zhao (Tianjin University of Science and Technology)H-Index: 2
#2Zhipeng Li (Tianjin University of Science and Technology)H-Index: 1
Last. Aipo Diao (Tianjin University of Science and Technology)H-Index: 10
view all 9 authors...
Abstract Legumain (LGMN) is a lysosomal protease that can specifically hydrolyze proteins after carboxyl-terminal asparagine residues. It has been reported that Legumain is highly expressed in many human tumors and promotes the migratory and invasive activity of cancer cells. Due to the limitation of an abundant and affordable source of endogenous active Legumain for further function studies, we produced the recombinant protein in Pichia pastoris. The pPICZα-LGMN expression plasmid was construct...
2 CitationsSource
#1Yuyin Li (Tianjin University of Science and Technology)H-Index: 8
#2Guojun Pan (Tianjin University of Science and Technology)H-Index: 4
Last. Peng Yu (Tianjin University of Science and Technology)H-Index: 13
view all 10 authors...
Abstract Telomerase is aberrantly expressed in many cancers and plays an important role in the development of cellular immortality and oncogenesis, which makes it a potential cancer therapeutic target for drug discovery. Here, we constructed a firefly luciferase reporter driven by the human telomerase reverse trancriptase ( hTERT ) gene promoter to screen for inhibitory compounds. Compound 5c was discovered and shown to significantly inhibit the promoter activity of hTERT gene. Furthermore, five...
12 CitationsSource
#1Long Ma (Tianjin University of Science and Technology)H-Index: 18
#2Nana Sun (Tianjin University of Science and Technology)H-Index: 5
Last. Shuli Man (Tianjin University of Science and Technology)H-Index: 16
view all 8 authors...
We report a novel assembly of polyethyleneimine (PEI)-coated Fe3O4 nanoparticles (NPs) with single-stranded DNA (ssDNA), and the fluorescence of the dye labeled in the DNA is remarkably quenched. In the presence of a target protein, the protein–DNA aptamer mutual interaction releases the ssDNA from this assembly and hence restores the fluorescence. This feature could be adopted to develop an aptasensor for protein detection. As a proof-of-concept, for the first time, we have used this proposed s...
9 CitationsSource
#1Zhenxing Liu (Tianjin University of Science and Technology)H-Index: 6
#2Xin Huo (Tianjin University of Science and Technology)H-Index: 1
Last. Aipo Diao (Tianjin University of Science and Technology)H-Index: 10
view all 11 authors...
Abstract LDLRAD4 was previously identified and shown to be connected with psychiatric disorders. The structure of LDLRAD4 protein is similar to that of TMEPAI protein, which is overexpressed in many tumors. However, it is still unknown whether LDLRAD4 is involved in tumorigenesis. In this study, the potential role of LDLRAD4 in tumorigenesis was investigated. LDLRAD4 is elevated in hepatic cancer cells and tumor tissues, and expression of LDLRAD4 promotes hepatic cancer cell HepG2 and SMMC-7721 ...
8 CitationsSource
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