Jiankui He
South University of Science and Technology of China
Publications 34
#1Deng Luo (SUSTech: South University of Science and Technology of China)
#2Meng Zhang (SUSTech: South University of Science and Technology of China)H-Index: 2
Last.Daru Lu (Fudan University)H-Index: 38
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The longest possible haplotype is chromosome haplotype that is a set of co-inherited alleles occurred on a single strand chromosome inherited from one parent. Standard whole-genome shotgun sequencing technologies are limited by the inability to independently study the haplotype of homologous chromosomes due to the short-reads sequencing strategy and disturbance of homologue chromosomes. Here, we investigated several types of chromosomal abnormalities by a dilution-based method to separate an int...
#1Muyun Peng (CSU: Central South University)
#2Yuancai Xie (PKU: Peking University)H-Index: 1
Last.Bing He (UM: University of Michigan)
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BACKGROUND: Early detection of lung cancer to allow curative treatment remains challenging. Cell-free circulating tumor DNA (ctDNA) analysis may aid in malignancy assessment and early cancer diagnosis of lung nodules found in screening imagery. METHODS: The multi-center clinical study enrolled 192 patients with operable occupying lung diseases. Plasma ctDNA, white blood cell genomic DNA (gDNA) and tumor tissue gDNA of each patient were analyzed by ultra-deep sequencing to an average of 35,000X o...
#1Yuancai Xie (PKU: Peking University)H-Index: 1
#2Fenglei Yu (CSU: Central South University)H-Index: 1
Last.Fengjun Cao (HUM: Hubei University of Medicine)H-Index: 1
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#1Jing He (Stanford University)H-Index: 1
#2H. Carlo MaurerH-Index: 2
Last.Jeanine M. Genkinger (Columbia University)H-Index: 20
view all 15 authors...
Bulk tumor tissues comprise intermixed populations of neoplastic cells and multiple stromal cell lineages. We used laser capture microdissection and RNA sequencing to disentangle the transcriptional programs active in the malignant epithelium and stroma of pancreatic ductal adenocarcinoma (PDA). This led to the development of a new algorithm (ADVOCATE) that accurately predicts the compartment fractions of bulk tumor samples and can computationally purify bulk gene expression data from PDA. We al...
#1Ligang Xia (JNU: Jinan University)H-Index: 2
#2Zhoufang Li (SUSTech: South University of Science and Technology of China)H-Index: 5
Last.Feiyue XuH-Index: 3
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Cell-free DNA (cfDNA) in plasma has emerged as a potential important biomarker in clinical diagnostics, particularly in cancer. However, somatic mutations are also commonly found in healthy individuals, which interfere with the effectiveness for cancer diagnostics. This study examined the background somatic mutations in white blood cells (WBC) and cfDNA in healthy controls based on sequencing data from 821 non-cancer individuals and several cancer samples with the aim of understanding the patter...
#1Geng TianH-Index: 1
#2Xiaohua LiH-Index: 3
Last.Shixin LuH-Index: 1
view all 14 authors...
Background: Remarkable advances for clinical diagnosis and treatment in cancers including lung cancer involve cell-free circulating tumor DNA (ctDNA) detection through next generation sequencing. However, before the sensitivity and specificity of ctDNA detection can be widely recognized, the consistency of mutations in tumor tissue and ctDNA should be evaluated. The urgency of this consistency is extremely obvious in lung cancer to which great attention has been paid to in liquid biopsy field. M...