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Kiyotaka Y. Hara
Kobe University
49Publications
17H-index
1,410Citations
Publications 49
Newest
#1Hideo Kawaguchi (Kobe University)H-Index: 17
#2Kumiko Yoshihara (Kobe University)H-Index: 2
Last.Akihiko Kondo (Kobe University)H-Index: 68
view all 6 authors...
Background l-Arabinose is the second most abundant component of hemicellulose in lignocellulosic biomass, next to d-xylose. However, few microorganisms are capable of utilizing pentoses, and catabolic genes and operons enabling bacterial utilization of pentoses are typically subject to carbon catabolite repression by more-preferred carbon sources, such as d-glucose, leading to a preferential utilization of d-glucose over pentoses. In order to simultaneously utilize both d-glucose and l-arabinose...
#1Alex Prima (LIPI: Indonesian Institute of Sciences)H-Index: 1
#2Kiyotaka Y. Hara (Kobe University)H-Index: 17
Last.Akihiko Kondo (Kobe University)H-Index: 68
view all 12 authors...
Abstract This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae . Mannan proved to be a valuable carbon source for glutathione production by this organism . Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, β-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a hig...
#1Jyumpei Kobayashi (Kobe University)H-Index: 2
#2Daisuke Sasaki (Kobe University)H-Index: 13
Last.Akihiko Kondo (Kobe University)H-Index: 68
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Background Oxidized glutathione (GSSG) is the preferred form for industrial mass production of glutathione due to its high stability compared with reduced glutathione (GSH). In our previous study, over-expression of the mitochondrial thiol oxidase ERV1 gene was the most effective for high GSSG production in Saccharomyces cerevisiae cells among three types of different thiol oxidase genes.
#1Kiyotaka Y. Hara (University of Shizuoka)H-Index: 17
#2Jyumpei Kobayashi (Kobe University)H-Index: 2
Last.Akihiko Kondo (Kobe University)H-Index: 68
view all 9 authors...
#1Kentaro Inokuma (Kobe University)H-Index: 11
#2Jun Ishii (Kobe University)H-Index: 20
Last.Akihiko Kondo (Kobe University)H-Index: 68
view all 6 authors...
Volume 3, no. 2, [e00389-15][1], 2015. Page 1, column 1, lines 26 and 27: “silencing mediator for retinoic acid and thyroid hormone receptor” should read “single-molecule real-time.” [1]: /lookup/doi/10.1128/genomeA.00389-15
#1Jun Ishii (Kobe University)H-Index: 20
#2Fumiyoshi Okazaki (Kobe University)H-Index: 10
Last.Prihardi Kahar (Kobe University)H-Index: 13
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Background Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced in sustainable biofuel development. Based on a yeast cell surface-display technology that enables the immobilization of multiple enzymes on the yeast cell walls, we constructed a recombinant Saccharomyces cerevisiae strain that co-displays β-mannanase and β-mannosidase; this strai...
#1Keisuke Yamamoto (Kobe University)H-Index: 2
#2Kiyotaka Y. Hara (Kobe University)H-Index: 17
Last.Akihiko Kondo (Kobe University)H-Index: 68
view all 9 authors...
Background Red yeast, Xanthophyllomyces dendrorhous is the only yeast known to produce astaxanthin, an anti-oxidant isoprenoid (carotenoid) widely used in the aquaculture, food, pharmaceutical and cosmetic industries. The potential of this microorganism as a platform cell factory for isoprenoid production has been recognized because of high flux through its native terpene pathway. Recently, we developed a multiple gene expression system in X. dendrorhous and enhanced the mevalonate synthetic pat...
#1Keiji Nishida (Kobe University)H-Index: 23
#2Takayuki Arazoe (Kobe University)H-Index: 6
Last.Kiyotaka Y. Hara (University of Shizuoka)H-Index: 17
view all 12 authors...
INTRODUCTION To combat invading pathogens, cells develop an adaptive immune response by changing their own genetic information. In vertebrates, the generation of genetic variation (somatic hypermutation) is an essential process for diversification and affinity maturation of antibodies that function to detect and sequester various foreign biomolecules. The activation-induced cytidine deaminase (AID) carries out hypermutation by modifying deoxycytidine bases in the variable region of the immunoglo...
#1Kengo Sasaki (Kobe University)H-Index: 16
#2Kiyotaka Y. Hara (Kobe University)H-Index: 17
Last.Akihiko Kondo (Kobe University)H-Index: 68
view all 6 authors...
This study aimed to optimize extracellular glutathione production by a Saccharomyces cerevisiae engineered strain and to concentrate the extracellular glutathione by membrane separation processes, including ultrafiltration (UF) and nanofiltration (NF). Synthetic defined (SD) medium containing 20 g L −1 glucose was fermented for 48 h; the fermentation liquid was passed through an UF membrane to remove macromolecules. Glutathione in this permeate was concentrated for 48 h to 545.1 ± 33.6 mg L −1 u...
#1Xiaoting Ye (Kobe University)H-Index: 4
#2Kana Morikawa (Kobe University)H-Index: 1
Last.Akihiko Kondo (Kobe University)H-Index: 68
view all 8 authors...
Background Measurement of mitochondrial ATP synthesis is a critical way to compare cellular energetic performance. However, fractionation of mitochondria requires large amounts of cells, lengthy purification procedures, and an extreme caution to avoid damaging intact mitochondria, making it the highest barrier to high-throughput studies of mitochondrial function. To evaluate 45 genes involved in oxidative phosphorylation in Saccharomyces cerevisiae, we aimed to develop a simple and rapid method ...
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