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Haiying Hang
Chinese Academy of Sciences
Cell cycle checkpointDNA damageMolecular biologyCell cycleBiology
66Publications
20H-index
1,558Citations
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Publications 64
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#1Ruiqi Luo (CAS: Chinese Academy of Sciences)H-Index: 1
#2Yun Zhao (CAS: Chinese Academy of Sciences)H-Index: 29
Last. Haiying Hang (CAS: Chinese Academy of Sciences)H-Index: 20
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Previously, we developed a CHO cell display-based antibody maturation procedure in which an antibody (or other protein) gene of interest was induced to mutate by activation-induced cytidine deaminase (AID) and then form a library by simply proliferating the CHO cells in culture. In this study, we further improved the efficiency of this maturation system by reengineering AID, and optimizing the nucleic acid sequence of the target antibody gene and AID gene as well as the protocol for AID gene tra...
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#1Yingjun FanH-Index: 1
Last. Haiying HangH-Index: 20
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#1Yingjun Fan (CAS: Chinese Academy of Sciences)H-Index: 1
#2Wei Jiang (CAS: Chinese Academy of Sciences)
Last. Haiying Hang (CAS: Chinese Academy of Sciences)H-Index: 20
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Antibodies have been extensively used for the purpose of scientific research, clinical diagnosis, and therapy. Combination of in vitro somatic hypermutation and mammalian cell surface display has been an efficient technology for antibody or other proteins optimization, in which the efficiency of activation-induced cytidine deaminase (AID) mutations in genes is one of the most important key factors. Gene transcriptional level has been found to be positively proportional to AID-induced mutation fr...
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#1Lili An (CAS: Chinese Academy of Sciences)H-Index: 8
#2Yanming Li (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Haiying Hang (CAS: Chinese Academy of Sciences)H-Index: 20
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The environment in space differs greatly from the environment on the ground. Spaceflight causes a number of physiological changes in astronauts, such as bone loss and immune system dysregulation. These effects threaten astronauts' space missions, and understanding the underlying cellular and molecular mechanisms is important to manage the risks of space missions. The biological effects of spaceflight on mammalian cells, especially with regards to DNA damage, have attracted much attention. Rad9-/...
1 CitationsSource
#1Jie Wang (CAS: Chinese Academy of Sciences)H-Index: 9
#2Lili An (CAS: Chinese Academy of Sciences)H-Index: 8
Last. Haiying Hang (CAS: Chinese Academy of Sciences)H-Index: 20
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G protein-coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, are among the most important targets against which many small molecule drugs have been developed. However, only two antibody drugs targeting GPCRs have been approved for clinical use although many antibody drugs against non-GPCR protein targets have been successfully developed for various disease indications. One of the challenges for developing anti-GPCR drugs is the high difficulty to perform affinity matu...
1 CitationsSource
#1Danni Wu (CAS: Chinese Academy of Sciences)H-Index: 4
#2Ning Zhang (CAS: Chinese Academy of Sciences)H-Index: 23
Last. Hailin Wang (CAS: Chinese Academy of Sciences)H-Index: 35
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Ultraviolet (UV) radiation could induce pyrimidine-related dimeric lesions in genomic DNA. Though the cyclobutane pyrimidine dimers (CPDs) are the most abundant UV-induced lesions, the pyrimidine (6-4) pyrimidone photoproducts (6-4PPs) may have more serious, potentially lethal, and mutagenic effects. It is important to have 6-4PP-containing oligodeoxynucleotides to be prepared for studying their adverse biological effects. Here, we developed a UV-irradiated water droplet method for the preparati...
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#1Lili An (CAS: Chinese Academy of Sciences)H-Index: 8
#2Yingjun Fan (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Haiying Hang (CAS: Chinese Academy of Sciences)H-Index: 20
view all 8 authors...
Because of the development of spacecraft design and life-support technologies, people can be sent farther in space and kept there longer. The characteristics of the space environment include the space radiation, microgravity, temperature extremes, high vacuum, space debris and ionospheric plasma etc. Among them, the two major challenges associated with spaceflight are the biological effects of space radiation and microgravity. DNA, the genetic material, is a kind of important biologic molecule. ...
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#1Chuan ChenH-Index: 2
#2Jie WangH-Index: 9
Last. Haiying HangH-Index: 20
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The induction of somatic hypermutation (SHM) in various cell lines by activation-induced cytidine deaminase (AID) has been used in protein-directed selection, especially in antibody affinity maturation. Several antibody affinity maturation systems based on mammalian cells have been developed in recent years, i.e., 293T, H1299, Raji and CHO cells. However, the efficiency of in vitro AID-induced hypermutation is low, restricting the application of such systems. In this study, we examined the role ...
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#1Zhishang HuH-Index: 7
#2Chen Ye (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Haiying Hang (CAS: Chinese Academy of Sciences)H-Index: 20
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The phenomenon of protein aggregation is a prominent challenge that impacts biopharmaceutical development at every stage. It may have a number of deleterious effects on protein drugs, including the loss of efficacy, induction of immunogenicity, altered pharmacokinetics and reduced shelf life. At present, multiple methods are available for counting and sizing particles over a broad range of sizes. However, there remains a conundrum in the measurement of particles in the submicrometer range, from ...
1 CitationsSource
#2Yuheng LiuH-Index: 6
Last. Haiying HangH-Index: 20
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Rad9 plays roles in both cell cycle checkpoint control and DNA repair. It can interact with the components of multiple DNA repair pathways and regulate their functions. Non-homologous end-joining (NHEJ) repair pathway is predominantly used in vertebrates for the repair of DNA double strand break (DSB). Proper activation of DNA-dependent protein kinase (DNA-PK), composed of Ku70, Ku80, and DNA-dependent protein kinase catalytic subunit (DNA-PKcs), is essential for NHEJ repair. In this study, we f...
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