Match!
Julian Lange
Memorial Sloan Kettering Cancer Center
16Publications
12H-index
887Citations
Publications 16
Newest
Published on 2018in Nature Communications11.88
Sarai Pacheco5
Estimated H-index: 5
(Autonomous University of Barcelona),
Andros Maldonado-Linares3
Estimated H-index: 3
(Autonomous University of Barcelona)
+ 7 AuthorsOscar Fernandez-Capetillo40
Estimated H-index: 40
Precise execution of recombination during meiosis is essential for forming chromosomally-balanced gametes. Meiotic recombination initiates with the formation and resection of DNA double-strand breaks (DSBs). Cellular responses to meiotic DSBs are critical for efficient repair and quality control, but molecular features of these remain poorly understood, particularly in mammals. Here we report that the DNA damage response protein kinase ATR is crucial for meiotic recombination and completion of m...
Published on 2018in Cell Cycle3.26
Agnieszka Lukaszewicz1
Estimated H-index: 1
(MSK: Memorial Sloan Kettering Cancer Center),
Julian Lange12
Estimated H-index: 12
(MSK: Memorial Sloan Kettering Cancer Center)
+ 1 AuthorsMaria Jasin11
Estimated H-index: 11
(MSK: Memorial Sloan Kettering Cancer Center)
ABSTRACTDNA double-strand breaks (DSBs) generated by the SPO11 protein initiate meiotic recombination, an essential process for successful chromosome segregation during gametogenesis. The activity of SPO11 is controlled by multiple factors and regulatory mechanisms, such that the number of DSBs is limited and DSBs form at distinct positions in the genome and at the right time. Loss of this control can affect genome integrity or cause meiotic arrest by mechanisms that are not fully understood. He...
Published on Dec 1, 2018in Nature Communications11.88
Alexander Widger1
Estimated H-index: 1
(Francis Crick Institute),
Shantha K. Mahadevaiah28
Estimated H-index: 28
(Francis Crick Institute)
+ 13 AuthorsObah A. Ojarikre19
Estimated H-index: 19
(Francis Crick Institute)
Meiotic cells undergo genetic exchange between homologs through programmed DNA double-strand break (DSB) formation, recombination and synapsis. In mice, the DNA damage-regulated phosphatidylinositol-3-kinase-like kinase (PIKK) ATM regulates all of these processes. However, the meiotic functions of the PIKK ATR have remained elusive, because germline-specific depletion of this kinase is challenging. Here we uncover roles for ATR in male mouse prophase I progression. ATR deletion causes chromosome...
Published on Oct 18, 2017in Cell Cycle3.26
Shintaro Yamada3
Estimated H-index: 3
(MSK: Memorial Sloan Kettering Cancer Center),
Seoyoung Kim3
Estimated H-index: 3
(MSK: Memorial Sloan Kettering Cancer Center)
+ 3 AuthorsScott Keeney47
Estimated H-index: 47
(MSK: Memorial Sloan Kettering Cancer Center)
ABSTRACTThe SPO11-generated DNA double-strand breaks (DSBs) that initiate meiotic recombination occur non-randomly across genomes, but mechanisms shaping their distribution and repair remain incompletely understood. Here, we expand on recent studies of nucleotide-resolution DSB maps in mouse spermatocytes. We find that trimethylation of histone H3 lysine 36 around DSB hotspots is highly correlated, both spatially and quantitatively, with trimethylation of H3 lysine 4, consistent with coordinated...
Published on Aug 30, 2017in PLOS Genetics5.22
Devanshi Jain2
Estimated H-index: 2
(MSK: Memorial Sloan Kettering Cancer Center),
Cem Meydan11
Estimated H-index: 11
(Cornell University)
+ 4 AuthorsScott Keeney47
Estimated H-index: 47
(MSK: Memorial Sloan Kettering Cancer Center)
Transcriptional silencing by heritable cytosine-5 methylation is an ancient strategy to repress transposable elements. It was previously thought that mammals possess four DNA methyltransferase paralogs—Dnmt1, Dnmt3a, Dnmt3b and Dnmt3l—that establish and maintain cytosine-5 methylation. Here we identify a fifth paralog, Dnmt3c, that is essential for retrotransposon methylation and repression in the mouse male germline. From a phenotype-based forward genetics screen, we isolated a mutant mouse cal...
Published on Nov 1, 2016in Nature Cell Biology17.73
Marcello Stanzione4
Estimated H-index: 4
,
Marek Baumann4
Estimated H-index: 4
+ 10 AuthorsYoshinori Watanabe51
Estimated H-index: 51
In meiosis, double-strand breaks (DSBs) are induced to initiate chromosome pairing and synapsis. Stanzione et al. identify IHO1 as a protein recruited by HORMAD1 to unsynapsed chromosome axes and required for DSB formation.
Published on Oct 1, 2016in Cell36.22
Julian Lange12
Estimated H-index: 12
(MSK: Memorial Sloan Kettering Cancer Center),
Shintaro Yamada3
Estimated H-index: 3
(MSK: Memorial Sloan Kettering Cancer Center)
+ 6 AuthorsScott Keeney47
Estimated H-index: 47
(Cornell University)
Summary Heritability and genome stability are shaped by meiotic recombination, which is initiated via hundreds of DNA double-strand breaks (DSBs). The distribution of DSBs throughout the genome is not random, but mechanisms molding this landscape remain poorly understood. Here, we exploit genome-wide maps of mouse DSBs at unprecedented nucleotide resolution to uncover previously invisible spatial features of recombination. At fine scale, we reveal a stereotyped hotspot structure—DSBs occur withi...
12