Giulio Cossu
University of Milan
306Publications
68H-index
20.1kCitations
Publications 306
Newest
Published on Jan 1, 2019in Molecular Therapy 7.01
Irene de Lázaro6
Estimated H-index: 6
(University of Manchester),
Açelya Yilmazer8
Estimated H-index: 8
(University of Manchester)
+ 5 AuthorsKostas Kostarelos62
Estimated H-index: 62
(University of Manchester)
Overexpression of Oct3/4 , Klf4 , Sox2 , and c-Myc (OKSM) transcription factors can de-differentiate adult cells in vivo . While sustained OKSM expression triggers tumorigenesis through uncontrolled proliferation of toti- and pluripotent cells, transient reprogramming induces pluripotency-like features and proliferation only temporarily, without teratomas. We sought to transiently reprogram cells within mouse skeletal muscle with a localized injection of plasmid DNA encoding OKSM (pOKSM), and we...
1 Citations Source Cite
Published on May 1, 2019in Journal of Cellular Physiology 3.92
Luca Ferrari29
Estimated H-index: 29
(University of Milan),
Cinzia Bragato7
Estimated H-index: 7
(University of Milano-Bicocca)
+ 14 AuthorsAntonio Giordano73
Estimated H-index: 73
(University of Siena)
1 Citations Source Cite
Published on Mar 1, 2019in Stem cell reports 6.54
Mattia F M Gerli6
Estimated H-index: 6
(University College London),
Louise A. Moyle6
Estimated H-index: 6
(University College London)
+ 11 AuthorsPierpaolo Ala1
Estimated H-index: 1
(University College London)
Summary Satellite cells are responsible for skeletal muscle regeneration. Upon activation, they proliferate as transient amplifying myoblasts, most of which fuse into regenerating myofibers. Despite their remarkable differentiation potential, these cells have limited migration capacity, which curtails clinical use for widespread forms of muscular dystrophy. Conversely, skeletal muscle perivascular cells have less myogenic potential but better migration capacity than satellite cells. Here we show...
1 Citations Source Cite
Published on Dec 1, 2018in Nature Communications 12.35
Luca Urbani13
Estimated H-index: 13
(University College London),
C Camilli2
Estimated H-index: 2
(University College London)
+ 28 AuthorsA Urciuolo3
Estimated H-index: 3
(University College London)
A tissue engineered oesophagus could overcome limitations associated with oesophageal substitution. Combining decellularized scaffolds with patient-derived cells shows promise for regeneration of tissue defects. In this proof-of-principle study, a two-stage approach for generation of a bio-artificial oesophageal graft addresses some major challenges in organ engineering, namely: (i) development of multi-strata tubular structures, (ii) appropriate re-population/maturation of constructs before tra...
3 Citations Source Cite
Published on Apr 5, 2018in Frontiers in Physiology 3.39
Eyemen Kheir (University of Trento), Gabriella Cusella4
Estimated H-index: 4
(University of Pavia)
+ 2 AuthorsStefano Biressi14
Estimated H-index: 14
(University of Trento)
The formation and activity of mammalian tissues entail finely regulated processes, involving the concerted organization and interaction of multiple cell types. In recent years the prospective isolation of distinct progenitor and stem cell populations has become a powerful tool in the hands of developmental biologists and has rendered the investigation of their intrinsic properties possible. In this protocol, we describe how to purify progenitors with different lineage history and degree of diffe...
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Published on Dec 1, 2018in Scientific Reports 4.12
Bashar Aldeiri2
Estimated H-index: 2
(Boston Children's Hospital),
Urmas Roostalu6
Estimated H-index: 6
(Manchester Academic Health Science Centre)
+ 4 AuthorsGiulio Cossu68
Estimated H-index: 68
(Manchester Academic Health Science Centre)
Pentalogy of Cantrell (PC) is a rare multi-organ congenital anomaly that impedes ventral body wall closure and results in diaphragmatic hernia, intra- and pericardial defects. The underlying cellular and molecular changes that lead to these severe developmental defects have remained unknown largely due to the lack of representative animal models. Here we provide in depth characterization of a mouse model with conditional ablation of TGFβRII in Transgelin (Tagln) expressing cells. We show that Ta...
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Published on Nov 1, 2018in Human Molecular Genetics 4.90
Ewa J. Mularczyk2
Estimated H-index: 2
(University of Manchester),
Mukti Singh2
Estimated H-index: 2
(University of Manchester)
+ 10 AuthorsRay Boot-Handford41
Estimated H-index: 41
(University of Manchester)
2 Citations Source Cite
Published on Jan 1, 2018in Cell Death and Disease 5.64
Cesare Gargioli15
Estimated H-index: 15
(University of Rome Tor Vergata),
Giuseppina Turturici8
Estimated H-index: 8
(University of Palermo)
+ 8 AuthorsGabriella Sconzo18
Estimated H-index: 18
(University of Palermo)
Cell engraftment, survival and integration during transplantation procedures represent the crux of cell-based therapies. Thus, there have been many studies focused on improving cell viability upon implantation. We used severe oxidative stress to select for a mouse mesoangioblast subpopulation in vitro and found that this subpopulation retained self-renewal and myogenic differentiation capacities while notably enhancing cell survival, proliferation and migration relative to unselected cells. Addi...
7 Citations Source Cite
Published on Nov 15, 2017in Biology Open 2.22
Artal Moreno-Fortuny2
Estimated H-index: 2
(Manchester Academic Health Science Centre),
Laricia Bragg1
Estimated H-index: 1
(Manchester Academic Health Science Centre)
+ 1 AuthorsUrmas Roostalu6
Estimated H-index: 6
(Manchester Academic Health Science Centre)
Cell polarity has a fundamental role in shaping the morphology of cells and growing tissues. Polarity is commonly thought to be established in response to extracellular signals. Here we used a minimal in vitro assay that enabled us to monitor the determination of cell polarity in myogenic and chondrogenic differentiation in the absence of external signalling gradients. We demonstrate that the initiation of cell polarity is regulated by melanoma cell adhesion molecule (MCAM). We found highly pola...
2 Citations Source Cite
Published on Dec 14, 2017in Embo Molecular Medicine 10.29
Sara Benedetti6
Estimated H-index: 6
(University College London),
Narumi Uno9
Estimated H-index: 9
(Tottori University)
+ 18 AuthorsSoraya Chaouch8
Estimated H-index: 8
(University of Paris)
Abstract Transferring large or multiple genes into primary human stem/progenitor cells is challenged by restrictions in vector capacity, and this hurdle limits the success of gene therapy. A paradigm is Duchenne muscular dystrophy (DMD), an incurable disorder caused by mutations in the largest human gene: dystrophin. The combination of large‐capacity vectors, such as human artificial chromosomes (HACs), with stem/progenitor cells may overcome this limitation. We previously reported amelioration ...
3 Citations Source Cite
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