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Yuanming Sun
South China Agricultural University
164Publications
21H-index
1,709Citations
Publications 136
Newest
#1Zhen-Lin Xu (SCAU: South China Agricultural University)H-Index: 20
#2Song-Ling Ye (SCAU: South China Agricultural University)
Last.Xing Shen (SCAU: South China Agricultural University)H-Index: 4
view all 11 authors...
Abstract In this work, a fluorescent nanoparticles labeling-free fluorescence enzyme-linked immunoassay (FELISA) has been established for the ultrasensitive detection of microcystin-LR (MC-LR) in water and fish samples. Polyclonal antibody against MC-LR was labeled with horseradish peroxidase (HRP) and used as signal probe for binding with analyte in sample or for coating antigen. After washing of the unbound antibody, the substrate system (2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS...
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#1Dong-Mei OuYang (SCAU: South China Agricultural University)
#2Jie Deng (SCAU: South China Agricultural University)
Last.Meiying Li (SCAU: South China Agricultural University)H-Index: 2
view all 9 authors...
Abstract Deacetylation was a phenomenon widely existed during konjac glucomannan (KGM) processing, which highly effected its functional properties. Herein, influence of deacetylation degree (DD) of KGM on microbial metabolites and gut microbiota was studied in vitro. The fermentability of KGM was relatively stable under the low (Da1) and medium (Da2) DD. But high DD (Da3) shown lower fermentability than that in KGM, which was characterized with the decreasing reducing sugar content, endo-β-manna...
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#1Ning Lu (SCAU: South China Agricultural University)H-Index: 1
#2Li Ling (SCAU: South China Agricultural University)
Last.Hongtao Lei (SCAU: South China Agricultural University)H-Index: 20
view all 11 authors...
Abstract A highly sensitive and broadly specific competitive indirect enzyme-linked immunosorbent assay (ciELISA) method was developed for the simultaneous detection of nine microcystins (MCs) and nodularin (NOD) using MC-LR-keyhole limpet hemocyanin (KLH) for New Zealand white rabbit immunization to produce antibodies. The MC-LR-bovine serum albumin (BSA) and NOD-BSA coating antigens were compared and heterogeneous coating strategy was found to significantly improve the sensitivity of detection...
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#1Tian GuanH-Index: 1
Last.Hongtao LeiH-Index: 20
view all 11 authors...
Establishing rapid, simple, and in situ detection of microcystin-LR (MC-LR) in drinking water sources is of significant importance for human health. To ease the situation that current methods cannot address, an open surface droplet microfluidic magnetosensor was designed and validated to quantify MC-LR in reservoir water, which is capable of (1) MC-LR isolation via MC-LR antibody-conjugated magnetic beads, (2) parallel and multistep analytical procedures in 15-array power-free and reusable activ...
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#1Hongting Liu (SCAU: South China Agricultural University)
#2Riming Huang (SCAU: South China Agricultural University)H-Index: 1
Last.Xiaoqun Wei (SCAU: South China Agricultural University)H-Index: 2
view all 10 authors...
Abstract A robust method for identification of reconstituted milk based on the content ratio of lactulose to furosine determined by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed and validated in the current study. The results showed that the optimal sample preparation conditions for hydrolysis were as follows: temperature 115 °C, time 4 h, and HCl concentration 11.9 mmol/L. The validated method was optimal for the limit of detection (LOD) (0.01 mg/L) and limi...
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#1Hui-Jun Fu (SCAU: South China Agricultural University)H-Index: 2
#2Yu WangH-Index: 4
Last.Bruce D. Hammock (UC Davis: University of California, Davis)H-Index: 98
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Abstract Tetrabromobisphenol A (TBBPA) is the largest brominated flame retardant which can be released to environment and cause long-term hazard. In this work, we developed a rapid and highly sensitive fluorescence enzyme-linked immunosorbent assay (FELISA) for monitoring of TBBPA in soil samples. TBBPA specific nanobody derived from camelid was fused with alkaline phosphatase to obtain the bi-functional fusion protein, which enable the specific binding of TBBPA and the generation of detection s...
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#1Xing ShenH-Index: 4
#2Jiahong ChenH-Index: 4
Last.Hongtao LeiH-Index: 20
view all 10 authors...
Enrofloxacin (ENR) is a widely used fluoroquinolone (FQ) antibiotic for antibacterial treatment of edible animal. In this study, a rapid and highly specific fluorescence polarization immunoassay (FPIA) was developed for monitoring ENR residues in animal foods. First, ENR was covalently coupled to bovine serum albumin (BSA) to produce specific polyclonal antibodies (pAbs). Three fluorescein-labeled ENR tracers (A, B, and C) with different spacers were synthesized and compared to obtain higher sen...
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#1Xiangmei Li (SCAU: South China Agricultural University)H-Index: 3
#2Xinze Wu (SCAU: South China Agricultural University)H-Index: 1
Last.Hongtao Lei (SCAU: South China Agricultural University)H-Index: 20
view all 8 authors...
Abstract In this study, colloidal gold (CG), latex microspheres (LM), and time-resolved fluorescent microsphere (TrFM), were utilized as the antibody labeled tracers in three kinds of lateral flow immunochromatographic assays (LFIAs), which were established to detect tylosin (TYL) and tilmicosin (TIM) residues in milk and pork. The cut-off values of the CG-LFIA, LM-LFIA, and TrFM-LFIA for TYL in milk were 8, 4, and 2 ng/mL, respectively, and that for pork were 15, 8, and 4 μg/kg, respectively. T...
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d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear. In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment. Furthermore, the underly...
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#1Ya Song (SCAU: South China Agricultural University)H-Index: 1
#2Minqian Zhu (SCAU: South China Agricultural University)
Last.Ri-Ming Huang (SCAU: South China Agricultural University)H-Index: 1
view all 9 authors...
Abstract A novel polysaccharide (PFP1) with an average molecular weight of 2.02 × 10 5 g/mol was isolated from Passiflora foetida fruits through hot water extraction, ethanol precipitation and column chromatography. The structure of PFP1 was determined by GPC-MALS-RI, IC, FT-IR, GC–MS and NMR. The structural analysis showed that PFP1 was a heteropolysaccharide and composed of mannose (48.83%), galactose (32.46%), glucose (6.21%), arabinose (5.88%), fructose (2.24%), galacturonic acid (2.20%), xy...
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