Sadia Saeed
Radboud University Nijmegen
Innate immune systemEpigeneticsGeneticsChromatinBiology
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Publications 16
#2C GiorgioH-Index: 3
Last. Lucia AltucciH-Index: 49
view all 15 authors...
Correction to: Leukemia (2017) 31, 2315–2325; doi:10.1038/leu.2017.64; published online 10 March 2017 Following the publication of this article, the authors noted a mistake in the author affiliations. 4. Centre for Research in Molecular Medicine, The University of Lahore, Lahore, Pakistan Should be replaced with:
5 CitationsSource
#1Daniel Rico (Newcastle University)H-Index: 24
#2Joost H.A. MartensH-Index: 30
Last. Hendrik G. StunnenbergH-Index: 89
view all 71 authors...
Neutrophils and monocytes provide a first line of defense against infections as part of the innate immune system. Here we report the integrated analysis of transcriptomic and epigenetic landscapes for circulating monocytes and neutrophils with the aim to enable downstream interpretation and functional validation of key regulatory elements in health and disease. We collected RNA-seq data, ChIP-seq of six histone modifications and of DNA methylation by bisulfite sequencing at base pair resolution ...
1 CitationsSource
#2C GiorgioH-Index: 3
Last. Lucia AltucciH-Index: 49
view all 15 authors...
Deregulation of epigenetic mechanisms, including microRNA, contributes to leukemogenesis and drug resistance by interfering with cancer-specific molecular pathways. Here, we show that the balance between miR-194-5p and its newly discovered target BCL2-associated transcription factor 1 (BCLAF1) regulates differentiation and survival of normal hematopoietic progenitors. In acute myeloid leukemias this balance is perturbed, locking cells into an immature, potentially /`immortal/' state. Enhanced ex...
21 CitationsSource
#1Charles E. Breeze (UCL: University College London)H-Index: 8
#2Dirk S. Paul (UCL: University College London)H-Index: 21
Last. Stephan Beck (UCL: University College London)H-Index: 81
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Epigenome-wide association studies (EWAS) provide an alternative approach for studying human disease through consideration of non-genetic variants such as altered DNA methylation. To advance the complex interpretation of EWAS, we developed eFORGE (, a new standalone and web-based tool for the analysis and interpretation of EWAS data. eFORGE determines the cell type-specific regulatory component of a set of EWAS-identified differentially methylated positions. This is a...
48 CitationsSource
#1Onkar Joshi (Radboud University Nijmegen)H-Index: 5
#2Shuang-Yin Wang (Radboud University Nijmegen)H-Index: 11
Last. Hendrik G. Stunnenberg (Radboud University Nijmegen)H-Index: 89
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Summary Serum-to-2i interconversion of mouse embryonic stem cells (mESCs) is a valuable in vitro model for early embryonic development. To assess whether 3D chromatin organization changes during this transition, we established Capture Hi-C with target-sequence enrichment of DNase I hypersensitive sites. We detected extremely long-range intra- and inter-chromosomal interactions between a small subset of H3K27me3 marked bivalent promoters involving the Hox clusters in serum-grown cells. Notably, t...
79 CitationsSource
#1Jessica QuintinH-Index: 22
#2Sadia SaeedH-Index: 11
Last. M.G. NeteaH-Index: 6
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#1Sadia Saeed (Radboud University Nijmegen)H-Index: 11
#2Jessica Quintin (Radboud University Nijmegen)H-Index: 22
Last. Hendrik G. Stunnenberg (Radboud University Nijmegen)H-Index: 89
view all 30 authors...
Introduction Monocytes circulate in the bloodstream for up to 3 to 5 days. Concomitantly, immunological imprinting of either tolerance (immunosuppression) or trained immunity (innate immune memory) determines the functional fate of monocytes and monocyte-derived macrophages, as observed after infection or vaccination. The epigenome, DNase I accessibility, and transcriptome were characterized in purified human circulating monocytes, in vitro differentiated naive, tolerized (immunosuppression), an...
499 CitationsSource
#1Shih-Chin Cheng (Radboud University Nijmegen)H-Index: 24
#2Jessica Quintin (Radboud University Nijmegen)H-Index: 22
Last. Mihai G. Netea (Radboud University Nijmegen)H-Index: 109
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To determine the epigenetic mechanisms that direct blood cells to develop into the many components of our immune system, the BLUEPRINT consortium examined the regulation of DNA and RNA transcription to dissect the molecular traits that govern blood cell differentiation. By inducing immune responses, Saeed et al. document the epigenetic changes in the genome that underlie immune cell differentiation. Cheng et al. demonstrate that trained monocytes are highly dependent on the breakdown of sugars i...
509 CitationsSource
#1Amit Mandoli (Radboud University Nijmegen)H-Index: 10
#2Abhishek A. SinghH-Index: 6
Last. Joost H.A. MartensH-Index: 30
view all 12 authors...
CBFB–MYH11/RUNX1 together with a compendium of hematopoietic regulators, chromatin modifiers and basal transcription factors occupies self-renewal genes in inv(16) acute myeloid leukemia
38 CitationsSource
#1Joost H.A. Martens (Radboud University Nijmegen)H-Index: 30
#2Amit Mandoli (Radboud University Nijmegen)H-Index: 10
Last. Hendrik G. Stunnenberg (Radboud University Nijmegen)H-Index: 89
view all 12 authors...